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Ssx2ip (NM_138744) Mouse Tagged ORF Clone
CAT＃: MG209435
- TrueORF®
Ssx2ip (tGFP-tagged) - Mouse synovial sarcoma, X breakpoint 2 interacting protein (Ssx2ip)
ORF Plasmid: DDK
"NM_138744" in other vectors (3)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Adip; AU014939; AU042321 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209435 representing NM_138744
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGATTGGATGACTGTGACAGATCCAGTTCTGTGTACAGAAAACAAAAATCTCTCTCAATATACCT CAGAAACAAAGATGTCTCCGTCCAGTTTGTACTCCCAGCAAGTTCTGTGCTCTTCAGTACCTTTATCCAA AAACGTGCATGGTGTTTTCGGTGTCTTCTGCACAGGAGAGAACATTGAACAAAGTATTTCCTATCTTGAT CAGGAGCTGACCACCTTCGGGTTTCCTTCCTTGTATGAAGAATCCAAAAGTAAAGAGGCAAAGAGAGAAT TAAATATAGTCGCTGTTCTGAACTGTATGAACGAGCTGCTCGTGCTTCAGCGGAAGAACTTGCTGGCCCA GGAGAGCGTGGAGACACAGAACTTGAAGCTGGGCAGTGACATGGACCACCTGCAGAGCTGCTACGCCAAA CTTAAGGAGCAGTTGGAAACGTCCAGGCGGGAGATGATCGGGCTTCAAGAGAGAGACAGGCAGCTGCAGT GCAAGAACAGGAGTTTGCATCAGCTCCTGAAGAATGAGAAAGATGAGGTACAAAAATTACAAAATATCAT AGCCAGCCGGGCTACTCAGTATAATCATGATGTGAAGAGGAAGGAGCGTGAATATAATAAGCTAAAGGAG CGCCTGCATCAGCTCGTTATGAACAAGAAGGATAAAAACATAGCCATGGATGTTTTAAATTATGTGGGTC GAGCTGATGGCAAACGAGGCTCATGGAGGACTGACAAAACAGAAGCCAGGAATGAAGATGAGATGTACAA AATTCTGTTGAATGATTATGAGTACCGCCAGAAGCAGATCCTGATGGAGAACGCGGAGCTGAAGAAGGTC CTCCAGCAGATGAAGAAGGAGATGATCTCTCTCCTGTCTCCTCAGAAGAAGAAGCCCAGGGAAAGAGCAG AGGACGGCACAGGCACTGTTGCTATCTCCGATATAGAAGATGACTCTGGGGAACTGAGCAGAGACAGCGT GTGGGGCCTTTCCTGTGACACTGTGAGAGAGCAGCTGACAAACAGCATCAGGAAACAGTGGAGAATTTTG AAAAGTCATGTAGAAAAACTCGATAACCAAGCTTCGAAGGTACACTCAGAGGGCCTTAATGAGGAGGACG TCATCTCACGACAAGACCATGAGCAAGAGACTGAGAAACTGGAGCTGGAGATTGAGCGGTGTAAAGAGAT GATCAAGGCTCAGCAGCAGCTCTTACAGCAGCAGCTGGCCACCACGTGTGATGATGACACCACCTCACTG TTGCGAGACTGTTACTTGCTGGAAGAAAAGGAACGCCTTAAAGAAGAGTGGACCCTTTTTAAAGAGCAAA AAAAGAATTTTGAGAGAGAAAGGCGAAGCTTTACAGAAGCTGCCATTCGATTGGGGTTGGAGAGAAAGGC GTTTGAAGAAGAGCGAGCCAGCTGGGTAAAGCAGCAGTTTTTAAACATGACGAACTTTGACCACCAGAAC TCAGAAAATGTGAAACTTTTCAGTGCTTTCTCAGGAAGTTCTGATCCAGACAATCTTATAGTCCACTCAC GGCCACGGCAAAAGAAGCTACACAGTGTGGCTAATGGGGTGCCAGCTTGCACATCAAAACTGACTAAATC TCTTCCTGCCTCACCTTCTACTTCAGACTTTCGCCAGACACATTCATGTGTGTCTGAACACAGTTCCATC AGTGTGCTGAATATAACTCCTGAAGAAAGTAAACCAAGTGAGGTTGCAAGAGAAAGCACGGATCAGAAGT GGAGCGTGCAGTCGAGGCCCAGCTCGCGGGAGGGGTGCTACAGCGGATGCTCCTCGGCCTTCAGGAGCGC TCACGGGGACCGAGATGACTTACCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209435 representing NM_138744
Red=Cloning site Green=Tags(s) MGDWMTVTDPVLCTENKNLSQYTSETKMSPSSLYSQQVLCSSVPLSKNVHGVFGVFCTGENIEQSISYLD QELTTFGFPSLYEESKSKEAKRELNIVAVLNCMNELLVLQRKNLLAQESVETQNLKLGSDMDHLQSCYAK LKEQLETSRREMIGLQERDRQLQCKNRSLHQLLKNEKDEVQKLQNIIASRATQYNHDVKRKEREYNKLKE RLHQLVMNKKDKNIAMDVLNYVGRADGKRGSWRTDKTEARNEDEMYKILLNDYEYRQKQILMENAELKKV LQQMKKEMISLLSPQKKKPRERAEDGTGTVAISDIEDDSGELSRDSVWGLSCDTVREQLTNSIRKQWRIL KSHVEKLDNQASKVHSEGLNEEDVISRQDHEQETEKLELEIERCKEMIKAQQQLLQQQLATTCDDDTTSL LRDCYLLEEKERLKEEWTLFKEQKKNFERERRSFTEAAIRLGLERKAFEEERASWVKQQFLNMTNFDHQN SENVKLFSAFSGSSDPDNLIVHSRPRQKKLHSVANGVPACTSKLTKSLPASPSTSDFRQTHSCVSEHSSI SVLNITPEESKPSEVARESTDQKWSVQSRPSSREGCYSGCSSAFRSAHGDRDDLP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138744 |
| ORF Size | 1845 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138744.1 |
| RefSeq Size | 3396 bp |
| RefSeq ORF | 1848 bp |
| Locus ID | 99167 |
| UniProt ID | Q8VC66 |
| Gene Summary | Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (PubMed:12446711). Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (PubMed:22027834). Involved in ciliogenesis (By similarity). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR209435 | Ssx2ip (Myc-DDK-tagged) - Mouse synovial sarcoma, X breakpoint 2 interacting protein (Ssx2ip) |
CNY 4,560.00 |
|
| MR209435L3 | Lenti ORF clone of Ssx2ip (Myc-DDK-tagged) - Mouse synovial sarcoma, X breakpoint 2 interacting protein (Ssx2ip) |
CNY 6,460.00 |
|
| MR209435L4 | Lenti ORF clone of Ssx2ip (mGFP-tagged) - Mouse synovial sarcoma, X breakpoint 2 interacting protein (Ssx2ip) |
CNY 6,460.00 |
