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Slc7a8 (NM_016972) Mouse Tagged ORF Clone
CAT＃: MG208519
- TrueORF®
Slc7a8 (tGFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8)
ORF Plasmid: DDK
"NM_016972" in other vectors (4)
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CNY 5,130.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA408822; LAT2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208519 representing NM_016972
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAAGGGAGCCCGCCAGCGAAACAACACCGCGAAGAACCACCCGGGTTCTGACACCAGCCCTGAGG CCGAGGCTAGCTCGGGAGGGGGCGGAGTAGCCCTGAAGAAAGAGATCGGATTGGTCAGCGCCTGTGGTAT CATTGTAGGGAACATCATTGGCTCCGGAATCTTTGTCTCACCGAAAGGTGTGCTGGAAAACGCGGGCTCT GTGGGCCTTGCTCTCATTGTCTGGATCGTGACAGGCATCATCACAGCCGTGGGAGCTCTCTGCTATGCTG AGCTAGGCGTCACCATCCCTAAGTCTGGAGGTGATTACTCTTATGTGAAGGACATCTTCGGAGGACTGGC TGGGTTCCTGCGGCTGTGGATTGCTGTGCTGGTGATCTACCCCACCAACCAAGCTGTCATCGCCCTCACC TTCTCCAACTACGTGCTGCAGCCGCTCTTCCCTACCTGCTTCCCCCCTGAGTCCGGTCTGCGACTCCTGG CTGCCATCTGTTTGTTGCTCCTCACATGGGTCAACTGCTCCAGTGTCCGATGGGCCACCCGGGTTCAAGA TATCTTCACAGCTGGGAAGCTCCTGGCCCTGGCTCTGATCATCATCATGGGTATTGTGCAGATATGCAAA GGAGAATTCTTCTGGCTGGAGCCAAAGAATGCATTTGAGAATTTCCAAGAACCTGACATCGGCCTCGTTG CTCTGGCATTCCTCCAGGGCTCCTTTGCCTATGGTGGCTGGAACTTCCTTAATTATGTGACTGAGGAACT TGTGGATCCTTACAAGAACCTTCCTAGAGCCATCTTCATCTCCATCCCACTGGTCACATTTGTGTACGTC TTTGCTAATATTGCATACGTCACTGCAATGTCCCCCCAGGAGCTGCTAGCCTCCAATGCAGTTGCTGTGA CTTTTGGAGAGAAACTCCTCGGGGTCATGGCCTGGATCATGCCCATTTCTGTTGCCCTGTCCACGTTTGG TGGAGTCAATGGCTCCCTCTTCACCTCCTCCCGGCTGTTCTTTGCTGGAGCCAGAGAAGGCCACCTTCCC AGTGTGTTGGCCATGATCCACGTGAAGCGCTGCACTCCAATCCCAGCCCTGCTCTTCACATGCCTCTCCA CCCTGCTGATGCTGGTCACCAGTGACATGTACACACTCATCAACTATGTGGGCTTCATCAACTACCTCTT CTATGGGGTAACGGTTGCAGGACAGATAGTCCTTCGCTGGAAGAAGCCTGACATTCCCCGCCCCATCAAG GTCAGCCTGCTGTTTCCCATTATCTACCTGCTGTTCTGGGCCTTCCTGCTGATCTTCAGCCTGTGGTCAG AGCCAGTGGTGTGTGGCATTGGCCTGGCCATTATGCTGACAGGAGTTCCTGTCTACTTCCTGGGTGTCTA CTGGCAACACAAACCCAAGTGTTTCAATGACTTCATTAAGTCCCTAACCCTAGTGAGTCAGAAGATGTGT GTGGTCGTGTATCCCCAGGAGGGGAACTCGGGGGCTGAGGAAACAACTGATGACTTAGAGGAGCAACACA AGCCCATCTTCAAGCCTACTCCTGTCAAGGACCCGGATTCGGAGGAGCAGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208519 representing NM_016972
Red=Cloning site Green=Tags(s) MEKGARQRNNTAKNHPGSDTSPEAEASSGGGGVALKKEIGLVSACGIIVGNIIGSGIFVSPKGVLENAGS VGLALIVWIVTGIITAVGALCYAELGVTIPKSGGDYSYVKDIFGGLAGFLRLWIAVLVIYPTNQAVIALT FSNYVLQPLFPTCFPPESGLRLLAAICLLLLTWVNCSSVRWATRVQDIFTAGKLLALALIIIMGIVQICK GEFFWLEPKNAFENFQEPDIGLVALAFLQGSFAYGGWNFLNYVTEELVDPYKNLPRAIFISIPLVTFVYV FANIAYVTAMSPQELLASNAVAVTFGEKLLGVMAWIMPISVALSTFGGVNGSLFTSSRLFFAGAREGHLP SVLAMIHVKRCTPIPALLFTCLSTLLMLVTSDMYTLINYVGFINYLFYGVTVAGQIVLRWKKPDIPRPIK VSLLFPIIYLLFWAFLLIFSLWSEPVVCGIGLAIMLTGVPVYFLGVYWQHKPKCFNDFIKSLTLVSQKMC VVVYPQEGNSGAEETTDDLEEQHKPIFKPTPVKDPDSEEQP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016972 |
| ORF Size | 1593 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016972.2, NP_058668.1 |
| RefSeq Size | 4064 bp |
| RefSeq ORF | 1596 bp |
| Locus ID | 50934 |
| UniProt ID | Q9QXW9 |
| Gene Summary | Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202413 | Slc7a8 (untagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8), (10ug) |
CNY 2,000.00 |
|
| MR208519 | Slc7a8 (Myc-DDK-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8) |
CNY 3,960.00 |
|
| MR208519L3 | Lenti ORF clone of Slc7a8 (Myc-DDK-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8) |
CNY 6,560.00 |
|
| MR208519L4 | Lenti ORF clone of Slc7a8 (mGFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8) |
CNY 6,560.00 |
