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Atp6v1h (NM_133826) Mouse Tagged ORF Clone
CAT＃: MG207742
- TrueORF®
Atp6v1h (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit H (Atp6v1h)
ORF Plasmid: DDK
"NM_133826" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 0710001F19Rik; AU022349; CGI-11; SFD; SFDalpha; SFDbeta; VMA13 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207742 representing NM_133826
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAAGATGGACATTCGAGGTGCTGTGGATGCTGCTGTCCCAACTAACATCATTGCGGCCAAGGCTG CTGAAGTTCGTGCCAACAAAGTGAACTGGCAGTCTTATCTTCAGGGGCAGATGATTTCTGCTGAAGATTG TGAATTTATTCAAAGGTTTGAAATGAAAAGAAGTTCCGAGGACAAGCAAGAGATGCTTCAAACTGAAGGC AGCCAGTGTGCTAAAACATTTATAAATCTGATGACGCACATCTCCAAAGAACAGACAGTGCAGTACATAC TAACCATGGTTGACGATATGCTGCAGGAAAATCACCAGCGTGTCAGCATTTTCTTTGACTATGCGAAACG AAGCAAGAGCACTGCCTGGCCATACTTCCTGCCAATGTTGAATCGCCAGGATCCCTTTACTGTTCATATG GCAGCAAGAATTATCGCCAAGTTAGCTGCTTGGGGGAAAGAACTGATGGAAGGCAGTGACTTAAATTACT ATTTCAATTGGATAAAAACTCAGCTGAGTTCACAGAAACTTCGTGGCAGCGGTGTTGCTGTTGAAACAGG AACAATCTCTTCAAGTGATAGTTCTCAGTATGTGCAGTGTGTTGCTGGCTGTCTGCAGCTGATGCTCCGG GTCAATGAGTACCGGTTTGCCTGGGTAGAAGCAGATGGAGTGAACTGTATAATGGGCGTTTTGAGTAACA AGTGTGGTTTTCAACTTCAATACCAAATGATTTTTTCAATATGGCTCCTGGCATTCAGTCCTCAAATGTG TGAGCACCTTCGTCGCTATAATATCATTCCTGTTCTATCAGACATCCTTCAAGAATCTGTCAAAGAGAAA GTAACAAGAATCATTCTTGCAGCATTCCGGAATTTCTTAGAAAAATCAACGGAAAGAGAAACTCGCCAAG AGTATGCTCTGGCTATGATTCAATGCAAAGTACTGAAACAGTTGGAGAATTTGGAGCAGCAGAAGTATGA TGATGAAGATATCAGTGAAGACATCAAATTTCTTTTGGAAAAACTTGGTGAGAGTGTCCAGGACCTTAGC TCATTTGATGAATACAGTTCAGAACTTAAATCTGGAAGATTGGAATGGAGCCCTGTACACAAGTCTGAGA AATTTTGGAGAGAAAATGCCGTGAGGTTGAATGAGAAGAACTATGAACTCTTGAAAATCTTGACAAAACT TCTGGAGGTATCAGATGATCCACAAGTCTTAGCTGTTGCTGCTCACGATGTTGGAGAATATGTCCGGCAT TATCCCCGGGGAAAACGGGTTATTGAGCAGCTTGGTGGAAAGCAGTTGGTGATGAACCACATGCACCATG AAGACCAGCAGGTTCGCTACAATGCTCTTCTGGCCGTGCAGAAGCTCATGGTGCACAACTGGGAATATCT TGGTAAACAACTACAGTCAGAGCAGCCTCAGACTGCTGCTGCTCGGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207742 representing NM_133826
Red=Cloning site Green=Tags(s) MTKMDIRGAVDAAVPTNIIAAKAAEVRANKVNWQSYLQGQMISAEDCEFIQRFEMKRSSEDKQEMLQTEG SQCAKTFINLMTHISKEQTVQYILTMVDDMLQENHQRVSIFFDYAKRSKSTAWPYFLPMLNRQDPFTVHM AARIIAKLAAWGKELMEGSDLNYYFNWIKTQLSSQKLRGSGVAVETGTISSSDSSQYVQCVAGCLQLMLR VNEYRFAWVEADGVNCIMGVLSNKCGFQLQYQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEK VTRIILAAFRNFLEKSTERETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLS SFDEYSSELKSGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRH YPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAAARS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133826 |
| ORF Size | 1449 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133826.5 |
| RefSeq Size | 1976 bp |
| RefSeq ORF | 1452 bp |
| Locus ID | 108664 |
| UniProt ID | Q8BVE3 |
| Gene Summary | Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200684 | Atp6v1h (untagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit H (Atp6v1h), (10ug) |
CNY 2,000.00 |
|
| MR207742 | Atp6v1h (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit H (Atp6v1h) |
CNY 3,330.00 |
|
| MR207742L3 | Lenti ORF clone of Atp6v1h (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit H (Atp6v1h) |
CNY 5,230.00 |
|
| MR207742L4 | Lenti ORF clone of Atp6v1h (mGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit H (Atp6v1h) |
CNY 5,230.00 |
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