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Pold2 (NM_008894) Mouse Tagged ORF Clone
CAT＃: MG207508
- TrueORF®
Pold2 (tGFP-tagged) - Mouse polymerase (DNA directed), delta 2, regulatory subunit (Pold2)
ORF Plasmid: DDK
"NM_008894" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 50kDa; p50; po1D2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207508 representing NM_008894
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCCGAGCAGGCAGCACAGAGGGCCCACACTCTGCTCGCCCCGCCCTCTGCCAGCAATGCCACCT TTGCCCGGGTACCTGTGGCAACTTACACCAACTCCTCACAGCCCTTCCGACTGGGAGAGCGCAGCTTTAA CCGGCAGTACGCCCATATTTATGCCACCCGCCTCATCCAGATGAGACCGTTCCTGGTGAGCCGGGCGCAG CAACACTGGGGCAGCCGAGTTGAAGTGAAGAAGTTGTGTGAGCTGCAGCCTGGGGAGCAGTGCTGTGTGG TGGGCACGCTGTTCAAAGCCATGTCCCTCCAACCCTCCATCCTGCGGGAGATCAGCGAGGAGCACAACCT GGTCCCCCAGCCTCCACGGAGCAAATATATCCACCCGGATGACGAGCTGGTCTTAGAAGATGAGCTGCAG CGTATCAAACTGAAAGGCACCATTGATGTGTCAAAGCTGGTCACAGGAACGGTCTTGGCCGTGTTGGGCT CTGCGAAAGATGACGGGAGGTTTCAGGTTGAAGACCACTGCTTTGCTGACCTGGCTCCACAGAAGCCGGT ACCCCCACTTGACACAGACAGATTTGTGCTACTGGTATCCGGACTGGGCCTGGGCGGTGGTGGCGGGGAG AGCCTCCTGGGCACCCAGCTGCTGGTGGACGTGGTGACGGGACAGCTTGGGGACGAAGGAGAACAGTGCA GTGCTGCCCACGTCTCTCGAGTCATCCTCGCAGGCAACCTGCTCAGCCATAACACCCAGAGCAGAGACTC CATCAACAAGGCCAAATACCTTACCAAGAAAACCCAGGCAGCCAGTGTGGAGGCAGTCAAAATGCTGGAC GAGATCCTTCTGCAACTGAGTGCCTCGGTACCGGTGGATGTGATGCCAGGCGAGTTTGATCCCACCAACT ATACACTCCCGCAGCAGCCCCTGCACCCCTGCATGTTCCCGCTGGCCACCGCCTACTCCACACTCCAGCT GGTCACCAACCCATACCAAGCCACCATTGATGGAGTAAGGTTCCTGGGGACATCTGGACAGAACGTGAGT GATATCTTCCGGTATAGCAGCATGGAAGACCACTTAGAGATCCTAGAGTGGACCCTGCGGGTTCGTCACA TCAGCCCCACGGCTCCAGACACCCTAGGGTGCTACCCCTTCTACAAAACCGACCCGTTCATCTTTCCGGA ATGTCCTCACGTCTACTTCTGTGGCAACACCCCCAGCTTTGGTTCCAAAATCATCCGAGGTCCTGAGGAC CAGGTAGTGCTGTTAGTGGCTGTTCCTGACTTCAGTTCCACACAGACGGCCTGCCTAGTGAACTTACGCA GCCTGGCCTGCCAGCCTATCAGCTTTGCGGGCTTCGGGGCAGAGCAAGAGGACCTGGAGGGCCTGGGGCT GGGTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207508 representing NM_008894
Red=Cloning site Green=Tags(s) MFSEQAAQRAHTLLAPPSASNATFARVPVATYTNSSQPFRLGERSFNRQYAHIYATRLIQMRPFLVSRAQ QHWGSRVEVKKLCELQPGEQCCVVGTLFKAMSLQPSILREISEEHNLVPQPPRSKYIHPDDELVLEDELQ RIKLKGTIDVSKLVTGTVLAVLGSAKDDGRFQVEDHCFADLAPQKPVPPLDTDRFVLLVSGLGLGGGGGE SLLGTQLLVDVVTGQLGDEGEQCSAAHVSRVILAGNLLSHNTQSRDSINKAKYLTKKTQAASVEAVKMLD EILLQLSASVPVDVMPGEFDPTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQATIDGVRFLGTSGQNVS DIFRYSSMEDHLEILEWTLRVRHISPTAPDTLGCYPFYKTDPFIFPECPHVYFCGNTPSFGSKIIRGPED QVVLLVAVPDFSSTQTACLVNLRSLACQPISFAGFGAEQEDLEGLGLGP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008894 |
| ORF Size | 1631 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008894.2, NP_032920.2 |
| RefSeq Size | 1564 bp |
| RefSeq ORF | 1410 bp |
| Locus ID | 18972 |
| UniProt ID | O35654 |
| Gene Summary | As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS as a component of the POLZ complex. Along with POLD3, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216220 | Pold2 (untagged) - Mouse polymerase (DNA directed), delta 2, regulatory subunit (Pold2), (10ug) |
CNY 6,080.00 |
|
| MR207508 | Pold2 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), delta 2, regulatory subunit (Pold2) |
CNY 3,330.00 |
|
| MR207508L3 | Lenti ORF clone of Pold2 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), delta 2, regulatory subunit (Pold2) |
CNY 5,230.00 |
|
| MR207508L4 | Lenti ORF clone of Pold2 (mGFP-tagged) - Mouse polymerase (DNA directed), delta 2, regulatory subunit (Pold2) |
CNY 5,230.00 |
