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Pisd (NM_177298) Mouse Tagged ORF Clone
CAT＃: MG206380
- TrueORF®
Pisd (tGFP-tagged) - Mouse phosphatidylserine decarboxylase (Pisd)
ORF Plasmid: DDK
"NM_177298" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9030221M09Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206380 representing NM_177298
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGTCTGGGGGCCGCGCGTGTGTGCGCTCGCTTCGTGGAGGTGTGCTGTGGCGGAGCAGCCCCT GTCACTATGAATCTACTGCCACACGCCATTTTCTGGGGACCTTACAGAAATTGCCTCTTCAGGCAGGTGT CAGAAATTTCCATACTGCTCCTGTCCGATCCTTGTTCCTGCTGCGCCCTGTGCCCATCCTGTTGGCAACA GGTGGAGGGTATGCCGGGTACCGGCAGTATGAGAAGTACAGGGAACGGAAGCTTGAGAAGCTGGGCTTGG AGATCCCGCCCAAACTTGCTAGTCACTGGGAGGTGTCCCTGTATAAATCTGTGCCAACGCGTTTGCTGTC ACGTGCCTGTGGTCGCCTCAACCAAGTAGAACTTCCTTACTGGCTCCGGAGGCCAGTCTACAGCCTGTAT ATCTGGACCTTTGGGGTGAACATGACTGAGGCTGCAGTGGAGGACCTGCACCACTACCGCAACCTCAGCG AGTTCTTCCGGCGTAAGCTGAAGCCTCAGGCACGGCCCGTATGTGGCTTGCACTGTGTGACCAGCCCATC AGATGGCAAGATCCTCACCTTTGGGCAGGTGAAGAACTCTGAGGTGGAGCAGGTAAAGGGCGTAACCTAC TCCTTGGAGTCCTTCCTGGGCCCTCGAGCCAATACAGAGGACCTGCCCTTCCCTCCAGCTTCCTCCAGTG ACTCCTTCAGAAACCAGCTGGTCACCCGGGAAGGGAATGAGCTGTACCACTGTGTCATCTACCTGGCCCC AGGAGACTACCACTGCTTCCACTCCCCCACTGACTGGACCATCTCACATCGGCGCCACTTCCCAGGTTCC CTGATGTCAGTGAACCCTGGCATGGCCCGATGGATCAAAGAGCTCTTTTGTCACAATGAGCGTGTAGTCC TAACTGGAGACTGGAAACATGGGTTCTTCTCGTTGACAGCAGTGGGAGCCACCAATGTGGGCTCTATCCG TATCCACTTTGACCGAGACCTGCACACAAACAGCCCCAGGTACAGCAAGGGCTCCTACAATGACCTGAGC TTTGTAACACATGCCAACAAGGAGGGCATTCCCATGCGCAAGGGTGAGCCCCTGGGGGAGTTCAACCTGG GCTCCACCATCGTGCTCATCTTCGAGGCACCCAAGGACTTCAACTTCAGGCTGAAGGCAGGGCAGAAGAT CCGCTTTGGGGAAGCACTGGGCTCCCTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206380 representing NM_177298
Red=Cloning site Green=Tags(s) MAASGGRACVRSLRGGVLWRSSPCHYESTATRHFLGTLQKLPLQAGVRNFHTAPVRSLFLLRPVPILLAT GGGYAGYRQYEKYRERKLEKLGLEIPPKLASHWEVSLYKSVPTRLLSRACGRLNQVELPYWLRRPVYSLY IWTFGVNMTEAAVEDLHHYRNLSEFFRRKLKPQARPVCGLHCVTSPSDGKILTFGQVKNSEVEQVKGVTY SLESFLGPRANTEDLPFPPASSSDSFRNQLVTREGNELYHCVIYLAPGDYHCFHSPTDWTISHRRHFPGS LMSVNPGMARWIKELFCHNERVVLTGDWKHGFFSLTAVGATNVGSIRIHFDRDLHTNSPRYSKGSYNDLS FVTHANKEGIPMRKGEPLGEFNLGSTIVLIFEAPKDFNFRLKAGQKIRFGEALGSL SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177298 |
| ORF Size | 1218 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177298.3, NP_796272.2 |
| RefSeq Size | 2150 bp |
| RefSeq ORF | 1221 bp |
| Locus ID | 320951 |
| UniProt ID | Q8BSF4 |
| Gene Summary | Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202609 | Pisd (untagged) - Mouse phosphatidylserine decarboxylase (Pisd), (10ug) |
CNY 2,000.00 |
|
| MR206380 | Pisd (Myc-DDK-tagged) - Mouse phosphatidylserine decarboxylase (Pisd) |
CNY 3,330.00 |
|
| MR206380L3 | Lenti ORF clone of Pisd (Myc-DDK-tagged) - Mouse phosphatidylserine decarboxylase (Pisd) |
CNY 5,230.00 |
|
| MR206380L4 | Lenti ORF clone of Pisd (mGFP-tagged) - Mouse phosphatidylserine decarboxylase (Pisd) |
CNY 5,230.00 |
