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Cavin1 (NM_008986) Mouse Tagged ORF Clone
CAT＃: MG206126
- TrueORF®
Ptrf (tGFP-tagged) - Mouse polymerase I and transcript release factor (Ptrf)
ORF Plasmid: DDK
"NM_008986" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310075E07Rik; AW546441; Cav-p60; Cavin |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206126 representing NM_008986
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATGTCACGCTCCATATCGTTGAGCGGCCGTATTCCGGATTTCCCGATGCTTCCTCAGAGGGCC CGGAGCCCACCCAAGGGGAGGCGCGGGCCACGGAGGAGCCGTCGGGGACCGGCTCCGACGAGCTGATCAA GTCGGACCAGGTGAACGGTGTGCTGGTGCTGAGCCTTCTGGATAAAATCATCGGCGCCGTTGACCAGATC CAGCTGACCCAAGCCCAGCTGGAGGAGCGACAGGCGGAGATGGAGGGCGCTGTGCAGAGCATCCAGGGAG AGCTGAGCAAGCTGGGCAAGGCGCACGCCACCACGAGCAACACCGTGAGCAAGTTGCTGGAGAAGGTGCG CAAGGTCAGCGTCAACGTGAAGACCGTGCGCGGCAGCCTGGAGCGCCAGGCCGGCCAGATAAAGAAACTG GAGGTCAACGAGGCGGAGCTGCTGAGGCGCCGCAACTTCAAAGTCATGATCTACCAGGATGAAGTCAAGC TGCCGGCCAAACTGAGCGTCAGCAAGTCGCTGAAAGAGTCGGAGGCACTGCCTGAGAAGGAGGGTGACGA GCTGGGCGAGGGCGAGCGGCCCGAGGATGACACCGCGGCGATCGAGCTGTCGTCCGACGAGGCGGTGGAG GTGGAGGAGGTGATCGAGGAGTCCCGCGCCGAGCGCATCAAGCGCAGCGGCCTGCGGCGCGTGGACGACT TCAAGAAGGCCTTCTCCAAGGAGAAGATGGAGAAGACCAAGGTGCGCACGCGTGAGAACCTGGAGAAGAC GCGCCTGAAGACCAAGGAGAACCTGGAGAAGACACGGCACACGCTGGAGAAGCGCATGAACAAGCTGGGC ACGCGCCTGGTGCCCGTGGAGCGACGAGAGAAGCTGAAGACATCCCGGGACAAGCTGCGCAAGTCCTTCA CGCCCGACCATGTGGTGTATGCGCGCTCCAAGACCGCTGTCTACAAGGTGCCGCCTTTCACCTTCCACGT CAAGAAGATCCGCGAGGGCGAGGTGGAGGTGCTGAAGGCCACCGAGATGGTGGAGGTGGGTCCCGAGGAC GACGAGGTTGGCGCGGAGCGCGGCGAGGCCACTGACCTGCTGCGCGGGAGCAGCCCCGACGTGCACACGC TGCTGGAGATCACCGAGGAGTCGGACGCCGTCCTGGTGGACAAGAGCGACAGCGAC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206126 representing NM_008986
Red=Cloning site Green=Tags(s) MEDVTLHIVERPYSGFPDASSEGPEPTQGEARATEEPSGTGSDELIKSDQVNGVLVLSLLDKIIGAVDQI QLTQAQLEERQAEMEGAVQSIQGELSKLGKAHATTSNTVSKLLEKVRKVSVNVKTVRGSLERQAGQIKKL EVNEAELLRRRNFKVMIYQDEVKLPAKLSVSKSLKESEALPEKEGDELGEGERPEDDTAAIELSSDEAVE VEEVIEESRAERIKRSGLRRVDDFKKAFSKEKMEKTKVRTRENLEKTRLKTKENLEKTRHTLEKRMNKLG TRLVPVERREKLKTSRDKLRKSFTPDHVVYARSKTAVYKVPPFTFHVKKIREGEVEVLKATEMVEVGPED DEVGAERGEATDLLRGSSPDVHTLLEITEESDAVLVDKSDSD SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008986 |
| ORF Size | 1176 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008986.2, NP_033012.1 |
| RefSeq Size | 3218 bp |
| RefSeq ORF | 1179 bp |
| Locus ID | 19285 |
| UniProt ID | O54724 |
| Gene Summary | Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues (PubMed:18191225, PubMed:18840361, PubMed:18056712, PubMed:30188967). Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1) (PubMed:19546242). Essential for normal oligomerization of CAV1 (PubMed:23652019). Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop (PubMed:27528195). Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template (PubMed:9582279, PubMed:11139612). The caveolae biogenesis pathway is required for the secretion of proteins such as GASK1A (PubMed:30188967).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207369 | Ptrf (untagged) - Mouse polymerase I and transcript release factor (Ptrf), (10ug) |
CNY 3,990.00 |
|
| MR206126 | Ptrf (Myc-DDK-tagged) - Mouse polymerase I and transcript release factor (Ptrf) |
CNY 2,850.00 |
|
| MR206126L3 | Lenti ORF clone of Ptrf (Myc-DDK-tagged) - Mouse polymerase I and transcript release factor (Ptrf) |
CNY 4,750.00 |
|
| MR206126L4 | Lenti ORF clone of Ptrf (mGFP-tagged) - Mouse polymerase I and transcript release factor (Ptrf) |
CNY 4,750.00 |
