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Phkg1 (NM_011079) Mouse Tagged ORF Clone
CAT＃: MG206062
- TrueORF®
Phkg1 (tGFP-tagged) - Mouse phosphorylase kinase gamma 1 (Phkg1)
ORF Plasmid: DDK
"NM_011079" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Phkg |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206062 representing NM_011079
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCGGGATGACGCCCTCCCTGACTCGCATTCTGCACAGACTTTCTACGAGAACTATGAGCCCAAGG AGATCCTGGGCAGGGGAGTCAGCAGTGTGGTCAGGCGCTGCATCCACAAACCCACATGCCAGGAATACGC AGTGAAAATCATTGACATCACCGGAGGAGGAAGCTTTAGCTCTGAGGAGGTACAGGAGCTTCGGGAAGCC ACCCTGAAGGAGGTGGACATCCTGCAGAAGGTCTCGGGACACCCCAACATCATACAGCTGAAGGACACTT ACGAGACCAACACTTTCTTCTTCTTGGTATTTGATCTGATGAAGAGAGGGGAACTCTTTGACTATCTCAC TGAGAAGGTCACCTTAACCGAGAAGGAAACCAGAAAGATCATGCGGGCCCTGCTGGAGGTGATCTGTACC CTGCACAAACTCAACATTGTCCATCGGGACCTGAAGCCGGAGAATATCCTTTTGGATGACAACATGAATA TAAAGCTCACAGACTTCGGGTTTTCCTGCCAGCTGCAGCCAGGAGAGAAGCTCCGAGAGGTTTGTGGGAC TCCCAGTTATCTGGCCCCTGAAATCATACAGTGCTCCATGGACGACGGCCATCCTGGCTATGGGAAGGAG GTGGACATGTGGAGCACAGGCGTCATCATGTACACTCTGCTGGCTGGCTCCCCGCCTTTCTGGCACCGGA AGCAAATGCTGATGTTGCGGATGATCATGGACGGCAAATACCAGTTTGGCTCACCAGAGTGGGATGACTA CTCTGACACAGTGAAAGACTTGGTGTCTCGATTCTTGGTGGTGCAACCTCAGGACCGCTGCTCGGCGGAA GAGGCCTTGGCACACCCTTTCTTTCAGGAGTACGTAGTAGAAGAAGTACGGCACTTCAGCCCCCGAGGGA AGTTCAAGGTGATCTGTCTAACTGTGCTGGCATCAGTAAAGATCTACTACCAGTACCGTCGGGTGAAGCC GGTAACCAGGGAGATCGTCATCCGAGACCCCTACGCCCTTCGGCCGCTGCGGAGGCTCATTGACGCCTAT GCTTTCCGTATCTACGGCCACTGGGTGAAGAAAGGGCAACAGCAGAACAGAGCCGCCCTCTTCGAGAACA CGCCCAAGGCTGTGCTCCTCTCCTTGGCTGAGGAGGAGGACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206062 representing NM_011079
Red=Cloning site Green=Tags(s) MTRDDALPDSHSAQTFYENYEPKEILGRGVSSVVRRCIHKPTCQEYAVKIIDITGGGSFSSEEVQELREA TLKEVDILQKVSGHPNIIQLKDTYETNTFFFLVFDLMKRGELFDYLTEKVTLTEKETRKIMRALLEVICT LHKLNIVHRDLKPENILLDDNMNIKLTDFGFSCQLQPGEKLREVCGTPSYLAPEIIQCSMDDGHPGYGKE VDMWSTGVIMYTLLAGSPPFWHRKQMLMLRMIMDGKYQFGSPEWDDYSDTVKDLVSRFLVVQPQDRCSAE EALAHPFFQEYVVEEVRHFSPRGKFKVICLTVLASVKIYYQYRRVKPVTREIVIRDPYALRPLRRLIDAY AFRIYGHWVKKGQQQNRAALFENTPKAVLLSLAEEEDF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011079 |
| ORF Size | 1164 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011079.3 |
| RefSeq Size | 2391 bp |
| RefSeq ORF | 1167 bp |
| Locus ID | 18682 |
| UniProt ID | P07934 |
| Gene Summary | Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205036 | Phkg1 (untagged) - Mouse phosphorylase kinase gamma 1 (Phkg1), (10ug) |
CNY 2,000.00 |
|
| MR206062 | Phkg1 (Myc-DDK-tagged) - Mouse phosphorylase kinase gamma 1 (Phkg1) |
CNY 2,850.00 |
|
| MR206062L3 | Lenti ORF clone of Phkg1 (Myc-DDK-tagged) - Mouse phosphorylase kinase gamma 1 (Phkg1) |
CNY 4,750.00 |
|
| MR206062L4 | Lenti ORF clone of Phkg1 (mGFP-tagged) - Mouse phosphorylase kinase gamma 1 (Phkg1) |
CNY 4,750.00 |
