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St8sia1 (NM_011374) Mouse Tagged ORF Clone
CAT＃: MG205419
- TrueORF®
St8sia1 (tGFP-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1)
ORF Plasmid: DDK
"NM_011374" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9330109E03Rik; GD3S; Sia-T; Siat8; Siat8a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205419 representing NM_011374
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCCTGCGGGCGGGCCCTACATACGTCCAGAGGGGCCATGGCCATGCTAGCTCGGAAATTCCCGC GTACCCGGCTACCCGTAGGAGCCAGTGCCCTCTGTGTGGTGGTCCTCTGTTGGCTCTACATCTTCCCTGT CTACCGGCTGCCCAACGAGAAGGAGATTGTGCAAGGGGTGCTGGCACAGCGGACGGCTTGGAGGACGAAC CAGACGTCAGCCAGTCTCTTCAGGAGACAAATGGAAGACTGCTGTGACCCTGCCCATCTCTTTGCTATGA CTAAGATGAACTCCCCCATGGGGAAGAGCCTGTGGTACGATGGGGAGCTCTTATACTCGTTCACCATCGA TAATTCCACGTACTCCCTCTTCCCCCAGGCAACCCCCTTCCAGCTGCCATTGAAGAAATGTGCGGTGGTG GGAAACGGTGGGATTCTGAAGATGAGTGGCTGTGGCCGTCAAATAGATGAAGCAAATTTTGTCATGCGGT GCAACCTGCCTCCCTTGTCAAGTGAGTACACCAGAGATGTCGGTTCCAAAACTCAGTTGGTGACAGCTAA CCCCAGCATAATTCGCCAGAGATTTGAAAACCTGCTGTGGTCCAGAAAGAAGTTTGTGGACAACATGAAG ATCTATAACCACAGTTACATCTACATGCCTGCCTTTTCTATGAAGACAGGCACAGAGCCGTCTCTCCGTG TGTATTACACACTGAAAGATGTTGGAGCCAATCAAACAGTGCTTTTTGCTAACCCCAACTTTCTTCGGAA CATTGGAAAGTTCTGGAAGAGTAGGGGGATCCACGCCAAGCGCTTGTCTACAGGACTCTTTTTGGTGAGT GCAGCCTTGGGCCTCTGTGAGGAGGTGTCCATCTATGGCTTCTGGCCCTTCTCGGTGAACATGCAAGGGG ACCCTATCAGTCACCACTACTATGACAACGTCTTGCCCTTCTCAGGCTACCATGCCATGCCTGAGGAATT CCTTCAGCTTTGGTATCTTCACAAAATCGGCGCGCTGAGGATGCAGCTGGACCCGTGTGAGGAGCCGTCA CCACAGCCCACTTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205419 representing NM_011374
Red=Cloning site Green=Tags(s) MSPCGRALHTSRGAMAMLARKFPRTRLPVGASALCVVVLCWLYIFPVYRLPNEKEIVQGVLAQRTAWRTN QTSASLFRRQMEDCCDPAHLFAMTKMNSPMGKSLWYDGELLYSFTIDNSTYSLFPQATPFQLPLKKCAVV GNGGILKMSGCGRQIDEANFVMRCNLPPLSSEYTRDVGSKTQLVTANPSIIRQRFENLLWSRKKFVDNMK IYNHSYIYMPAFSMKTGTEPSLRVYYTLKDVGANQTVLFANPNFLRNIGKFWKSRGIHAKRLSTGLFLVS AALGLCEEVSIYGFWPFSVNMQGDPISHHYYDNVLPFSGYHAMPEEFLQLWYLHKIGALRMQLDPCEEPS PQPTS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011374 |
| ORF Size | 1065 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011374.2, NP_035504.2 |
| RefSeq Size | 8995 bp |
| RefSeq ORF | 1068 bp |
| Locus ID | 20449 |
| UniProt ID | Q64687 |
| Gene Summary | Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204123 | St8sia1 (untagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1), (10ug) |
CNY 2,000.00 |
|
| MR205419 | St8sia1 (Myc-DDK-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1) |
CNY 3,330.00 |
|
| MR205419L3 | Lenti ORF clone of St8sia1 (Myc-DDK-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1) |
CNY 5,230.00 |
|
| MR205419L4 | Lenti ORF clone of St8sia1 (mGFP-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1) |
CNY 5,230.00 |
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