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Cnot9 (NM_021383) Mouse Tagged ORF Clone
CAT＃: MG204171
- TrueORF®
Rqcd1 (tGFP-tagged) - Mouse rcd1 (required for cell differentiation) homolog 1 (S
ORF Plasmid: DDK
"NM_021383" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610007F23Rik; AI593551; Fl10; Rqcd1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204171 representing NM_021383
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACAGCCTGGCAACGGCAGCGCCTGTGCCTACTGCACTAGCCCAAGTGGACAGAGAGAAGATCTATC AGTGGATCAATGAACTGTCCAGTCCTGAGACAAGGGAAAATGCTTTGTTGGAGCTGAGCAAGAAGAGAGA GTCTGTCCCTGACCTTGCACCCATGCTATGGCATTCATTTGGTACTATTGCAGCACTGTTACAGGAAATT GTAAATATTTATCCATCTATCAACCCCCCAACCTTGACAGCACACCAGTCTAACAGAGTTTGCAATGCTT TAGCATTGCTGCAGTGTGTGGCCTCACACCCGGAGACCAGGTCAGCTTTTCTGGCAGCACACATCCCACT CTTTTTGTACCCCTTTTTGCACACAGTCAGCAAAACTCGTCCCTTTGAATATCTTCGGCTCACCAGCCTT GGAGTTATTGGGGCCTTGGTTAAAACAGATGAGCAAGAAGTAATCAACTTTTTATTGACCACAGAGATCA TCCCTCTGTGTCTGCGCATCATGGAGTCTGGAAGTGAACTCTCTAAAACGGTTGCCACATTCATACTCCA GAAGATCCTCTTGGATGACACTGGTTTAGCTTATATATGTCAGACATATGAGCGTTTTTCCCATGTTGCC ATGATCTTGGGTAAAATGGTCCTGCAGCTATCCAAAGAACCGTCAGCCCGTCTGCTGAAGCACGTAGTAA GATGTTACCTTCGACTCTCAGATAATCCCAGGGCACGTGAAGCACTCAGACAGTGCCTCCCTGACCAGCT GAAGGACACAACCTTTGCCCAGGTGCTAAAAGACGACACCACCACGAAACGCTGGCTTGCACAACTGGTG AAGAACCTGCAAGAGGGCCAGGTCACCGATCCCCGGGGGATTCCCCTGCCCCCTCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204171 representing NM_021383
Red=Cloning site Green=Tags(s) MHSLATAAPVPTALAQVDREKIYQWINELSSPETRENALLELSKKRESVPDLAPMLWHSFGTIAALLQEI VNIYPSINPPTLTAHQSNRVCNALALLQCVASHPETRSAFLAAHIPLFLYPFLHTVSKTRPFEYLRLTSL GVIGALVKTDEQEVINFLLTTEIIPLCLRIMESGSELSKTVATFILQKILLDDTGLAYICQTYERFSHVA MILGKMVLQLSKEPSARLLKHVVRCYLRLSDNPRAREALRQCLPDQLKDTTFAQVLKDDTTTKRWLAQLV KNLQEGQVTDPRGIPLPPQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021383 |
| ORF Size | 897 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021383.5 |
| RefSeq Size | 3267 bp |
| RefSeq ORF | 900 bp |
| Locus ID | 58184 |
| UniProt ID | Q9JKY0 |
| Gene Summary | Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in down-regulation of MYB- and JUN-dependent transcription. May play a role in cell differentiation. Required for retinoic acid-induced differentiation of F9 teratocarcinoma cells. Does not bind DNA by itself. Enhances ligand-dependent transcriptional activity of nuclear hormone receptors. May play a role in cell differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202003 | Rqcd1 (untagged) - Mouse rcd1 (required for cell differentiation) homolog 1 (S. pombe) (Rqcd1), (10ug) |
CNY 2,000.00 |
|
| MR204171 | Rqcd1 (Myc-DDK-tagged) - Mouse rcd1 (required for cell differentiation) homolog 1 (S. pombe) (Rqcd1) |
CNY 1,416.00 |
|
| MR204171L3 | Lenti ORF clone of Rqcd1 (Myc-DDK-tagged) - Mouse rcd1 (required for cell differentiation) homolog 1 (S. pombe) (Rqcd1) |
CNY 4,750.00 |
|
| MR204171L4 | Lenti ORF clone of Rqcd1 (mGFP-tagged) - Mouse rcd1 (required for cell differentiation) homolog 1 (S. pombe) (Rqcd1) |
CNY 4,750.00 |
