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Alkbh3 (NM_026944) Mouse Tagged ORF Clone
CAT＃: MG203896
- TrueORF®
Alkbh3 (tGFP-tagged) - Mouse alkB, alkylation repair homolog 3 (E. coli) (Alkbh3)
ORF Plasmid: DDK
"NM_026944" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1700108H04Rik; 1810020C19Rik; Abh3; mABH3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203896 representing NM_026944
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACAAGAGACAGCGAGCCCGAGTGCAGGGAGGCTGGGCTACACCTACCAAGAGCCAGTCTGCTA CTCAGCCAGCTTCCCCTGCTAGGAGCCGTCTTTCCCAGACCGCGGGGCCAGCCTGGAGGAGCAAGGAACA GCAGCAATGTGACCGACAATTTGTGTTCAAAGAACCTCAGCTGGTTGTCCGTGCAGCCCCAGAACCCAGA GTGATTGACAGGGAGGGTGTGTATGAAATCAGCCTGTCACCTACTGGTGTGTCTAGGGTGTGCTTATATC CTGGCTTTGTGGACTTGAAGGAAGCTGACTGGATCTTGGAGCAGCTTTGTAAGGATGTCCCCTGGAAACA GAGGATGGGCATCAGAGAGGATGTAACTTATCCGCAACCAAGACTTACAGCATGGTATGGAGAGCTTCCT TACACTTACTCGAGAATCACTATGGAACCAAATCCTCACTGGCTTCCTGTGCTGTGGACTCTGAAGAGCC GCATTGAAGAGAACACCAGCCACACCTTCAACTCCTTGCTGTGTAATTTTTACCGGGATGAGAAGGACAG TGTGGACTGGCACAGCGACGATGAACCATCCCTGGGGAGCTGCCCCGTCATTGCTTCCCTCAGTTTTGGT GCCACTCGGACTTTTGAGATGAGGAAGAAGCCCCCACCTGAAGAAAATGGAGACTATACATATGTGGAGA GAGTGAAGATACCATTGGATCACGGGACCTTGCTAATCATGGAAGGAGCCACACAAGCCGACTGGCAGCA CCGAGTGCCCAAGGAATACCACTCCAGACAACCGAGAGTAAACTTAACCTTTCGGACCGTTTATCCAGAC CCAAGAGGAGCCCCTCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203896 representing NM_026944
Red=Cloning site Green=Tags(s) MEDKRQRARVQGGWATPTKSQSATQPASPARSRLSQTAGPAWRSKEQQQCDRQFVFKEPQLVVRAAPEPR VIDREGVYEISLSPTGVSRVCLYPGFVDLKEADWILEQLCKDVPWKQRMGIREDVTYPQPRLTAWYGELP YTYSRITMEPNPHWLPVLWTLKSRIEENTSHTFNSLLCNFYRDEKDSVDWHSDDEPSLGSCPVIASLSFG ATRTFEMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSRQPRVNLTFRTVYPD PRGAPR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026944 |
| ORF Size | 858 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026944.1, NP_081220.1 |
| RefSeq Size | 1247 bp |
| RefSeq ORF | 861 bp |
| Locus ID | 69113 |
| UniProt ID | Q8K1E6 |
| Gene Summary | Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (By similarity). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:16174769). Has a strong preference for single-stranded DNA (PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205847 | Alkbh3 (untagged) - Mouse alkB, alkylation repair homolog 3 (E. coli) (Alkbh3), (10ug) |
CNY 2,000.00 |
|
| MR203896 | Alkbh3 (Myc-DDK-tagged) - Mouse alkB, alkylation repair homolog 3 (E. coli) (Alkbh3) |
CNY 2,850.00 |
|
| MR203896L3 | Lenti ORF clone of Alkbh3 (Myc-DDK-tagged) - Mouse alkB, alkylation repair homolog 3 (E. coli) (Alkbh3) |
CNY 4,750.00 |
|
| MR203896L4 | Lenti ORF clone of Alkbh3 (mGFP-tagged) - Mouse alkB, alkylation repair homolog 3 (E. coli) (Alkbh3) |
CNY 4,750.00 |
