United States
Germany
Japan
United Kingdom
China
Dnase1 (NM_010061) Mouse Tagged ORF Clone
CAT＃: MG203857
- TrueORF®
Dnase1 (tGFP-tagged) - Mouse deoxyribonuclease I (Dnase1)
ORF Plasmid: DDK
"NM_010061" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI788650; DNaseI; Dnl1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203857 representing NM_010061
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGTACACAGGGCTAATGGGAACACTGCTCACCTTGGTCAACCTGCTGCAGCTGGCTGGGACTCTGA GAATTGCAGCCTTCAACATTCGGACTTTTGGGGAGACTAAGATGTCCAATGCTACCCTCTCTGTATACTT TGTGAAAATCCTGAGTCGCTATGACATCGCTGTTATCCAAGAGGTCAGAGACTCCCACCTGGTTGCTGTT GGGAAGCTCCTGGATGAACTCAATCGGGACAAACCTGACACCTACCGCTATGTAGTCAGTGAGCCGCTGG GCCGTAAAAGCTACAAGGAACAGTACCTTTTTGTGTACAGGCCTGACCAGGTGTCTATTCTGGACAGCTA TCAATATGATGATGGCTGTGAACCCTGTGGAAATGACACCTTCAGCAGAGAGCCAGCCATTGTTAAGTTC TTTTCCCCATACACTGAGGTCCAAGAATTTGCGATCGTGCCCTTGCATGCAGCCCCAACAGAAGCTGTGA GTGAGATCGACGCCCTCTACGATGTTTACCTAGATGTCTGGCAAAAGTGGGGCCTGGAGGACATCATGTT CATGGGAGACTTCAATGCTGGCTGCAGCTACGTCACTTCCTCCCAGTGGTCCTCCATTCGCCTTCGGACA AGCCCCATCTTCCAGTGGCTGATCCCTGACAGTGCGGACACCACAGTCACATCAACACACTGTGCTTATG ACAGGATTGTGGTTGCTGGAGCTCTGCTCCAGGCTGCTGTTGTTCCCAACTCGGCTGTTCCTTTTGATTT CCAAGCAGAATACAGACTTTCCAACCAGCTGGCTGAAGCCATCAGTGACCATTACCCAGTGGAGGTGACA CTCAGAAAAATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203857 representing NM_010061
Red=Cloning site Green=Tags(s) MRYTGLMGTLLTLVNLLQLAGTLRIAAFNIRTFGETKMSNATLSVYFVKILSRYDIAVIQEVRDSHLVAV GKLLDELNRDKPDTYRYVVSEPLGRKSYKEQYLFVYRPDQVSILDSYQYDDGCEPCGNDTFSREPAIVKF FSPYTEVQEFAIVPLHAAPTEAVSEIDALYDVYLDVWQKWGLEDIMFMGDFNAGCSYVTSSQWSSIRLRT SPIFQWLIPDSADTTVTSTHCAYDRIVVAGALLQAAVVPNSAVPFDFQAEYRLSNQLAEAISDHYPVEVT LRKI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010061 |
| ORF Size | 852 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010061.4, NP_034191.2 |
| RefSeq Size | 1312 bp |
| RefSeq ORF | 855 bp |
| Locus ID | 13419 |
| UniProt ID | P49183 |
| Gene Summary | Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed:29191910). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (PubMed:29191910). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed:29191910). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (PubMed:29191910).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201502 | Dnase1 (untagged) - Mouse deoxyribonuclease I (Dnase1), (10ug) |
CNY 2,000.00 |
|
| MR203857 | Dnase1 (Myc-DDK-tagged) - Mouse deoxyribonuclease I (Dnase1) |
CNY 1,416.00 |
|
| MR203857L3 | Lenti ORF clone of Dnase1 (Myc-DDK-tagged) - Mouse deoxyribonuclease I (Dnase1) |
CNY 4,750.00 |
|
| MR203857L4 | Lenti ORF clone of Dnase1 (mGFP-tagged) - Mouse deoxyribonuclease I (Dnase1) |
CNY 4,750.00 |
