Ctdspl (BC094289) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	SCP3, HYA22
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203704 representing BC094289 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGTCCGGCCATCATCACCCAGGTGACCAACCCCAAGGAGGATGAGGCCCGGTCGCCCGTCGCGG GCGAAAAAGCTTCCCAGCGCAACATCAGCCTAAAGAAGCAAAGGGGTCGGAGCATCCTTAGCTCTTTCTT CTGCTGCTTCCGAGACTACAATGTGGAAGCCCCTCCGGCCAACAGCCCCAGTGTGCTTCCGCCACTGGTG GAGGAGAACGGTGGGCTTCAGAAGGGTGACCAGAGGCAGGTCATTCCCGTACCAAGCCCACCAGCTAAAT ACCTCCTTCCAGAGGTGACGGTGCTCGACTATGGAAAGAAATGTGTGGTCATTGATCTAGACGAAACCCT GGTGCACAGTTCGTTTAAGCCCATTAGTAATGCTGACTTCATTGTTCCTGTTGAAATCGACGGAACCATA CACCAGGTGTATGTGCTGAAGCGACCTCACGTGGACGAGTTCCTCCAGAGGATGGGGCAGCTCTTTGAGT GTGTGCTCTTTACTGCCAGCTTGGCCAAGTATGCAGACCCTGTTGCCGACCTCCTGGACCGCTGGGGTGT GTTCCGGGCCCGACTCTTCAGAGAATCCTGCGTGTTCCACCGTGGGAACTATGTGAAGGACTTGAGTCGC CTGGGACGGGAGCTGAGCAAAGTGATCATCGTGGACAATTCTCCTGCCTCCTACATCTTCCATCCTGAGA ATGCAGTGCCCGTGCAGTCCTGGTTTGACGACATGACAGACACGGAGCTGTTGGACCTCATCCCGTTCTT CGAGGGCCTGAGCAGGGAAGACGATGTCTACAGCATGCTGCACAGACTCTGTAGCAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203704 representing BC094289 Red=Cloning site Green=Tags(s) MDGPAIITQVTNPKEDEARSPVAGEKASQRNISLKKQRGRSILSSFFCCFRDYNVEAPPANSPSVLPPLV EENGGLQKGDQRQVIPVPSPPAKYLLPEVTVLDYGKKCVVIDLDETLVHSSFKPISNADFIVPVEIDGTI HQVYVLKRPHVDEFLQRMGQLFECVLFTASLAKYADPVADLLDRWGVFRARLFRESCVFHRGNYVKDLSR LGRELSKVIIVDNSPASYIFHPENAVPVQSWFDDMTDTELLDLIPFFEGLSREDDVYSMLHRLCSR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC094289
ORF Size	830 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC094289, AAH94289
RefSeq Size	1051 bp
RefSeq ORF	830 bp
Locus ID	69274
Gene Summary	Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity).[UniProtKB/Swiss-Prot Function]

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