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Psmb8 (NM_010724) Mouse Tagged ORF Clone
CAT＃: MG203694
- TrueORF®
Psmb8 (tGFP-tagged) - Mouse proteosome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) (Psmb8)
ORF Plasmid: DDK
"NM_010724" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Lmp-7; Lmp7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203694 representing NM_010724
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTACTGGATCTGTGCGGTGCCGCTCGGGGGCAGCGGCCCGAGTGGGCTGCCCTGGATGCGGGAA GCGGGGGTCGCTCGGACCCGGGACACTACAGTTTCTCCGCGCAAGCTCCGGAGCTCGCACTTCCCCGGGG AATGCAGCCCACCGCATTCCTGAGGTCCTTTGGTGGTGACCAGGAAAGGAATGTTCAAATTGAGATGGCC CACGGCACAACCACACTCGCCTTCAAGTTCCAGCATGGCGTCATCGTGGCTGTGGACTCCAGGGCCACTG CAGGGAGTTACATTAGCTCCTTAAGGATGAACAAAGTGATCGAGATTAACCCTTACCTGCTTGGCACCAT GTCTGGTTGTGCAGCCGACTGCCAGTACTGGGAGAGGCTGTTGGCCAAGGAGTGCAGGTTGTATTATCTT CGGAATGGGGAACGCATCTCCGTGTCTGCAGCATCCAAGCTGCTTTCCAACATGATGCTGCAGTACCGGG GGATGGGCCTCTCCATGGGCAGCATGATCTGTGGCTGGGACAAGAAGGGACCAGGACTTTACTACGTAGA TGACAATGGGACTCGGCTCTCGGGACAGATGTTTTCCACTGGCAGCGGGAACACCTATGCCTACGGGGTG ATGGACAGTGGTTACCGGCAGGACCTCAGTCCTGAAGAGGCCTACGACCTTGGCCGCAGAGCTATTGCTT ATGCTACCCACAGAGACAACTATTCTGGAGGAGTCGTCAACATGTACCATATGAAGGAAGACGGTTGGGT GAAAGTGGAGAGTTCCGATGTCAGTGACCTGCTGTACAAGTACCGAGAGGCCGCTCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203694 representing NM_010724
Red=Cloning site Green=Tags(s) MALLDLCGAARGQRPEWAALDAGSGGRSDPGHYSFSAQAPELALPRGMQPTAFLRSFGGDQERNVQIEMA HGTTTLAFKFQHGVIVAVDSRATAGSYISSLRMNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYL RNGERISVSAASKLLSNMMLQYRGMGLSMGSMICGWDKKGPGLYYVDDNGTRLSGQMFSTGSGNTYAYGV MDSGYRQDLSPEEAYDLGRRAIAYATHRDNYSGGVVNMYHMKEDGWVKVESSDVSDLLYKYREAAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010724 |
| ORF Size | 831 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010724.1, NP_034854.1 |
| RefSeq Size | 828 bp |
| RefSeq ORF | 831 bp |
| Locus ID | 16913 |
| UniProt ID | P28063 |
| Gene Summary | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. May participate in the inflammatory response pathway. Required for adipocyte differentiation (PubMed:21881205, PubMed:22341445, PubMed:8066463). May be involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201912 | Psmb8 (untagged) - Mouse proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) (Psmb8), (10ug) |
CNY 2,000.00 |
|
| MR203694 | Psmb8 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) (Psmb8) |
CNY 1,416.00 |
|
| MR203694L3 | Lenti ORF clone of Psmb8 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) (Psmb8) |
CNY 4,750.00 |
|
| MR203694L4 | Lenti ORF clone of Psmb8 (mGFP-tagged) - Mouse proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) (Psmb8) |
CNY 4,750.00 |
