Ywhaq (BC090838) Mouse Tagged ORF Clone
CAT#: MG203062
- TrueORF®
Ywhaq (tGFP-tagged) - Mouse tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (cDNA clone MGC:102094
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CNY 2,850.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 14-3-3theta, RP23-402H11.1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203062 representing BC090838
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGACCGAGCTGATCCAGAAGGCCAAGCTGGCCGAGCAGGCCGAGCGCTACGACGACATGGCCA CCTGCATGAAAGCCGTGACGGAGCAGGGCGCCGAGCTGTCCAACGAGGAGCGCAACCTGCTGTCGGTGGC CTACAAAAACGTGGTAGGGGGCCGCAGGTCCGCCTGGAGGGTCATCTCGAGCATTGAGCAGAAGACCGAC ACCTCTGACAAGAAGTTGCAGCTGATCAAGGACTATCGGGAGAAAGTGGAGTCGGAGCTGAGGTCCATCT GCACCACGGTCCTGGAATTGTTGGATAAGTATTTAATAGCCAATGCAACTAATCCAGAGAGTAAGGTCTT CTATCTGAAAATGAAGGGAGATTATTTCCGGTATCTTGCTGAAGTAGCTTGTGGCGATGATCGAAAACAA ACAATAGAAAATTCCCAAGGAGCCTACCAAGAGGCGTTTGATATAAGCAAGAAGGAGATGCAGCCTACGC ATCCAATCCGCCTGGGGCTGGCTCTTAACTTTTCTGTATTTTACTATGAGATCCTTAATAATCCAGAGCT TGCCTGCACACTGGCTAAAACGGCTTTTGATGAGGCCATCGCAGAGCTTGATACACTGAACGAAGACTCC TACAAAGACAGCACCCTCATCATGCAGTTGCTTAGAGACAACTTAACATTATGGACATCAGACAGTGCAG GAGAAGAATGTGATGCAGCAGAGGGGGCCGAAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203062 representing BC090838
Red=Cloning site Green=Tags(s) MEKTELIQKAKLAEQAERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTD TSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQ TIENSQGAYQEAFDISKKEMQPTHPIRLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDS YKDSTLIMQLLRDNLTLWTSDSAGEECDAAEGAEN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC090838 |
ORF Size | 737 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC090838, AAH90838 |
RefSeq Size | 2130 bp |
RefSeq ORF | 737 bp |
Locus ID | 22630 |
Gene Summary | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |