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Asf1a (NM_025541) Mouse Tagged ORF Clone
CAT＃: MG202116
- TrueORF®
Asf1a (tGFP-tagged) - Mouse ASF1 anti-silencing function 1 homolog A (S
ORF Plasmid: DDK
"NM_025541" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310079C17Rik; C79052 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202116 representing NM_025541
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGGTTCAGGTGAACAATGTAGTGGTGCTGGATAACCCGTCGCCTTTCTACAACCCGTTCCAGT TCGAGATCACCTTCGAGTGCATCGAGGACCTGTCTGAAGACTTGGAGTGGAAAATAATCTATGTGGGCTC TGCAGAAAGTGAAGAATACGATCAAGTTTTAGACTCTGTTTTAGTGGGTCCCGTTCCTGCAGGAAGGCAT ATGTTTGTGTTTCAGGCTGATGCACCGAATGCAGGACTCATCCCAGATGCAGATGCAGTGGGGGTAACAG TTGTTCTGATTACTTGCACCTACCGAGGTCAAGAATTTATTAGAGTTGGCTACTATGTAAATAATGAATA CACTGAAACAGAATTAAGGGAAAACCCACCAGTAAAACCAGACTTTTCTAAGCTTCAAAGGAATATTTTG GCATCTAATCCCAGAGTCACAAGATTCCACATTAACTGGGAAGATAATACAGAAAAACTGGAAGATGCAG AGAGCAGTAACCCAAATCTACAGTCCCTTCTTTCAACAGATGCATTACCGTCAGCGTCAAAGGGATGGTC CACGTCAGAAAACTCACTCAATGTTATGTTAGAATCCCACATGGACTGCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202116 representing NM_025541
Red=Cloning site Green=Tags(s) MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRH MFVFQADAPNAGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNIL ASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025541 |
| ORF Size | 612 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025541.3, NP_079817.1 |
| RefSeq Size | 2192 bp |
| RefSeq ORF | 615 bp |
| Locus ID | 66403 |
| UniProt ID | Q9CQE6 |
| Gene Summary | Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201350 | Asf1a (untagged) - Mouse ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (Asf1a), (10ug) |
CNY 2,000.00 |
|
| MR202116 | Asf1a (Myc-DDK-tagged) - Mouse ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (Asf1a) |
CNY 1,416.00 |
|
| MR202116L3 | Lenti ORF clone of Asf1a (Myc-DDK-tagged) - Mouse ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (Asf1a) |
CNY 4,750.00 |
|
| MR202116L4 | Lenti ORF clone of Asf1a (mGFP-tagged) - Mouse ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (Asf1a) |
CNY 4,750.00 |
