P2rx7 (NM_001284402) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI467586; P2X(7); P2X7R
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228402 representing NM_001284402 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCCGTGAGCCACTTATGCAGCCATAAATCCGGGAAAGTCCTTGAAATCCATAGCATATGGATCG GGACGCTGAAGAACACCTTCCTAGGGGCCATCTGCGTCTATATCTGCTTTGCTTTGGTGAGCGATAAGCT GTACCAGCGGAAAGAGCCTGTTATCAGCTCCGTGCACACCAAGGTCAAAGGCATAGCAGAGGTGACGGAG AATGTCACAGAGGGTGGGGTGACGAAGTTAGGACACAGCATCTTTGACACTGCAGACTACACCTTCCCTT TGCAGGGGAACTCATTCTTTGTCATGACAAACTATGTCAAGTCAGAAGGCCAAGTGCAGACGCTGTGTCC TGAGTATCCCAGGCGCGGTGCACAGTGCTCTTCTGACCGGCGTTGTAAAAAGGGGTGGATGGACCCACAG AGCAAAGGAATCCAGACTGGCAGGTGTGTTCCATATGACAAGACAAGGAAGACCTGTGAAGTCTCTGCCT GGTGTCCTACTGAGGAGGAGAAAGAAGCTCCCCGACCTGCACTCTTGAGGAGCGCCGAAAACTTCACCGT ACTCATCAAGAATAATATCCACTTCCCCGGCCACAACTATACCACGAGAAACATCTTGCCAACTATGAAC GGCTCTTGTACCTTTCACAAGACTTGGGACCCTCAGTGTTCCATCTTCCGACTAGGGGACATCTTCCAGG AAGCAGGAGAGAACTTTACAGAGGTGGCAGTTCAGGGAGGAATCATGGGTATCGAGATCTACTGGGATTG CAACCTAGACAGCTGGTCCCATCACTGCCGACCCAGGTACAGCTTCCGCCGCCTGGATGACAAGAACACG GATGAGTCCTTCGTCCCCGGCTACAACTTCAGATATGCCAAGTACTATAAGGAGAACAATGTGGAAAAGC GGACGTTGATCAAAGCCTTCGGCATCCGTTTTGACATCCTGGTTTTCGGCACTGGAGGAAAATTTGACAT CATCCAGCTGGTTGTATACATTGGATCCACCCTGTCCTACTTTGGCTTGGCCACTGTGTGCATTGACTTG CTCATCAACACATACTCCAGTGCTTTCTGCAGGTCGGGGGTTTACCCCTACTGTAAGTGCTGTGAGCCCT GCACAGTGAACGAGTATTACTACAGAAAGAAGTGTGAGTCCATCATGGAACCCAAGCCGACGTTGAAGTA TGTGTCCTTTGTCGACGAGCCGCACATTCGCATGGTGGACCAGCAGCTGCTTGGGAAAAGTCTGCAAGTT GTCAAAGGCCAAGAAGTTCCAAGACCCCAGATGGACTTCTCCGACCTGTCTAGGCTGTCCCTATCTCTCC ACGACTCACCCCTGACTCCTGGACAATCTGAGGAAATTCAGCTGCTCCATGAAGAGGTGGCCCCTAAGTC CGGGGACAGCCCGAGTTGGTGCCAGTGTGGAAATTGCCTCCCGTCTCGCCTACCGGAGCAACGCAGGGCC CTGGAGGAGCTGTGCTGCCGGAGGAAGCCGGGGCGGTGCATCACCACCTCCAAGCTCTTCCATAAGCTCG TGCTGTCCCGAGACACCCTGCAGCTCCTCCTGCTTTACCAGGATCCCTTGCTGGTGCTGGGGGAAGAGGC CACCAACAGCAGGCTGCGACACCGTGCTTACAGGTGCTATGCCACCTGGCGCTTCGGCTCCCAGGACATG GCCGACTTTGCCATTCTGCCCAGCTGTTGCCGCTGGAGGATCCGGAAGGAGTTCCCCAAGACCGAGGGGC AGTATAGTGGCTTCAAGTATCCCTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284402
Insert Size	1779 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284402.1, NP_001271331.1
RefSeq Size	4764 bp
RefSeq ORF	1779 bp
Locus ID	18439
Gene Summary	Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) includes an alternate 5' terminal exon and differs in the 5' UTR and the 5' coding region, compared to variant 1. It encodes isoform e, which is shorter and has a distinct N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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