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Sephs2 (NM_009266) Mouse Untagged Clone
CAT＃: MC203276
Sephs2 (untagged) - Mouse selenophosphate synthetase 2 (Sephs2), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)
CNY 1,936.00
CNY 2,000.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Synonyms | S; Sps2; Ysg; Ysg3 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC028966
GGTCCGGCTCGCGTGGTTGATCATCTCCTGGCGTAACCTTGGCCCGCTGTGGCTGGGAGACTCATCTGCA GGTATCTGGGCCTTCTGGTCCGCACGGCCTCCCGGGCGAGCGGCGCGATGGCGGAAGCGGCGGCGGCGGG CGCCAGCGGAGAAACCATGGCGGCGCTAGTGGCCGCAGAAGGTTCCTTGGGCCCGGCGGGCTGGTCTGCC GGCCGGAGTTTCTCCAACTACCGGCCGTTCGAGCCCCAGACACTGGGCTTCAGCCCGAGCTGGCGGCTGA CGAGCTTCTCCGGCATGAAGGGCTGAGGCTGCAAGGTCCCCCAGGAGACCCTGCTCAAACTCCTGGAGGG ACTGACGCGGCCCGCGCTGCAGCCCCCGCTTACCTCGGGTCTGGTCGGGGGCCAGGAAGAGACGGTGCAG GAAGGGGGCCTGTCCACCAGGCCCGGCCCCGGCTCAGCCTTCCCCTCGCTGAGCATTGGCATGGACTCCT GCGTCATCCCCCTGAGGCACGGAGGCCTGTCGCTGGTGCAGACCACCGACTTCTTTTACCCCTTGGTGGA AGATCCCTATATGATGGGGCGCATAGCTTGTGCCAATGTGCTCAGTGACCTCTATGCCATGGGTATCACT GAGTGTGACAACATGTTGATGTTACTCAGTGTGAGCCAGAGCATGAGTGAAAAGGAACGAGAGAAGGTGA CACCGCTCATGATCAAAGGCTTTCGTGACGCTGCGGAGGAGGGAGGCACTGCAGTGACCGGTGGACAGAC AGTGGTCAACCCGTGGATTATCATCGGTGGCGTTGCCACGGTGGTGTGTCAGCAAAATGAATTCATAATG CCTGATAGTGCCGTGGTAGGAGATGTGCTGGTATTAACCAAACCTTTAGGAACCCAGGTTGCTGCCAATG CCCACCAATGGCTGGATAATCCTGAGAAATGGAATAAAATCAAGATGGTGGTTTCCAGAGAGGAAGTAGA GTTAGCCTATCAGGAAGCTATGTTCAATATGGCTACTCTAAACAGGACTGCTGCTGGCTTGATGCACACT TTTAATGCCCACGCAGCCACGGATATCACAGGCTTTGGCATATTAGGACACTCTCAGAACCTGGCAAAAC AGCAAAAAAATGAAGTGTCCTTTGTCATACATAATCTGCCAATAATTGCCAAGATGGCTGCGATCAGCAA AGCCAGTGGGCGCTTTGGCCTCCTCCAAGGAACGTCAGCTGAAACCTCTGGGGGATTACTGATTTGTCTG CCAAGAGAGCAGGCGGCCCGCTTTTGTTCGGAAATCAAATCTTCCAAGTACGGAGAGGGTCACCAAGCTT GGATCGTTGGCATCGTGGAGAAGGGAAACCGGACAGCCCGGATCATTGACAAGCCTCGCGTTATTGAAGT TCTACCTCGGGGAGCCTCTGCTGCTGCTGCTGCTGCTCCTGATAATTCCAACGCAGCCTCTGAGCCTAGT TCTTGAAATGGAATAGCGGTTGTTGGGAACTCGGAGCCATTCTACCCGCTCAGGGACTGCTGGCCAGGGT TGATTTTAAGACCTTTCCAAAGGCTGCTTGCATGGTTCCTCCAGGCCCATCCAAAGCTTCCTGTATGTGC ATCCAGGCCTGTGAGTAATGGCGCTGCGGATGTGTGTTCATCTGTTGAGAGCATGAGGAGCAAAAACCCG TTTCCCAAAGCAAGAGGAGGCTATTTCAGTTTTAGGGATTTTTTTTTTTTTTTTTTTTTGCACTGAGTTG ATTCATTTCTGCACAGGGAGTAAAGATTATTAAGATTACATATGAGAAAAGTAAACCTGCAACATGAAAA ATTATTTGGACCAATATATTGATAAATCTAAATTGTTAGGAGAACTCTTACTGATTTATTGTCAAATTTG TTATTAATTTTTTTCTGAGAAACTGCCTCTTTTCCTGTTCTGGACAAGAGTTGAGCAGCTTGTCCGACAG GAAAGGAAGACTAGCCACCTGACTTGGTCTCTGATAATGATGTCTCTCCCTCTAACTCCCAGTAAGGACT GGGAGAGGCTGAACAAACCTCAGAGCCAGGTGTCGGTGGCCATTGAATCTTACACTGAAACTTCTGGAGA TTTAATTAATAAAGAGAATTTCTTACAGTAACTAAATAAAAGGGACTTTGTTGGAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_009266 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins. |
| OTI Annotation | This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC028966, AAH28966 |
| RefSeq Size | 2208 bp |
| Locus ID | 20768 |
| UniProt ID | P97364 |
| Gene Summary | This gene encodes an enzyme that catalyzes the production of monoselenophosphate (MSP) from selenide and ATP. MSP is the selenium donor required for synthesis of selenocysteine (Sec), which is co-translationally incorporated into selenoproteins at in-frame UGA codons that normally signal translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is itself a selenoprotein containing a Sec residue at its active site, suggesting the existence of an autoregulatory mechanism. It is preferentially expressed in tissues implicated in the synthesis of selenoproteins and in sites of blood cell development. [provided by RefSeq, May 2017] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207218 | Sephs2 (GFP-tagged) - Mouse selenophosphate synthetase 2 (Sephs2), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 4,370.00 |
|
| MR207218 | Txnrd1 (Myc-DDK-tagged) - Mouse thioredoxin reductase 1 (Txnrd1), transcript variant 4, (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 3,656.00 |
|
| MR207225 | Txnrd2 (Myc-DDK-tagged) - Mouse thioredoxin reductase 2 (Txnrd2), nuclear gene encoding mitochondrial protein, (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 3,816.00 |
