United States
Germany
Japan
United Kingdom
China
Stk3 (NM_019635) Mouse Untagged Clone
CAT＃: MC202665
Stk3 (untagged) - Mouse serine/threonine kinase 3 (Ste20, yeast homolog) (Stk3), (10ug)
CNY 2,000.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 0610042I06Rik; mess1; MST; Mst2; Mst3; Ste20 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC049123 sequence for NM_019635
CGGAGTTACGTGAAAGTTGGTTCGAGTTTCCCGGTGTGATCCCTTGCGTTCGGCCGGCTGCTCCCGGGCT CCTTTCCTCCCTACGGCCTTCACGGTCCATTCGGCCCTTCTTTCTGCCACCGGCCGCCATGGAGCAGCCG CCGGCGTCCAAGAGTAAGCTAAAAAAGCTGAGTGAAGACAGTTTGACTAAGCAGCCTGAAGAAGTTTTTG ATGTACTGGAGAAGCTTGGAGAAGGGTCTTATGGAAGTGTTTTTAAAGCAATACATAAGGAATCTGGTCA AGTGGTTGCAATTAAGCAAGTACCTGTTGAGTCAGATCTTCAGGAAATAATCAAAGAAATTTCCATAATG CAACAATGTGACAGTCCATATGTTGTGAAGTACTATGGCAGTTACTTTAAGAACACAGACCTCTGGATTG TTATGGAGTACTGTGGAGCGGGGTCCGTTTCAGACATAATTAGATTGCGAAACAAGACATTAACAGAAGA TGAAATTGCAACTATTCTAAAATCCACATTGAAAGGATTAGAATATTTGCATTTTATGAGGAAAATACAC AGAGATATAAAAGCCGGGAATATTCTCCTCAATACAGAAGGACATGCAAAGCTTGCAGATTTTGGAGTGG CTGGCCAGTTAACAGATACAATGGCAAAACGCAACACTGTAATAGGAACCCCATTTTGGATGGCTCCTGA GGTAATTCAAGAAATAGGTTACAACTGTGTGGCTGACATCTGGTCCCTTGGCATTACTTCTATAGAAATG GCAGAAGGAAAACCTCCTTATGCTGATATACATCCGATGAGGGCTATTTTTATGATCCCTACAAACCCAC CACCAACATTCAGGAAACCTGAACTTTGGTCTGATGACTTCACCGATTTTGTGAAGAAGTGCTTGGTGAA GAGTCCTGAGCAGAGAGCCACTGCGACACAGCTGTTACAGCATCCTTTTATCAAGAATGCAAAACCCGTG TCGATATTAAGAGACCTGATCGCAGAAGCCATGGAAATCAAAGCCAAACGGCATGAAGAGCAGCAGCGGG AGCTAGAGGAGGAGGAAGAAAACTCGGATGAAGATGAGCTGGATTCACACACTATGGTGAAGACGAGTTC AGAAAGCGTGGGCACAATGCGGGCCACCAGCACGATGAGTGAAGGAGCCCAGACGATGATTGAACATAAC AGCACCATGTTAGAGTCGGACCTGGGGACCATGGTTATAAACAGTGAAGAAGAGGAGGAAGAGGAAGAGG AGGAGGAGGAGGACGGAACTATGAAAAGAAATGCAACGTCACCTCAAGTACAAAGACCTTCTTTCATGGA CTACTTTGATAAGCAGGACTTCAAGAACAAGAGTCATGAAAATTGTGATCAGAGCATGCGTGAGCCAGGC CCTATGTCCAACAGTGTTTTTCCTGACAACTGGAGAGTTCCTCAAGATGGAGACTTTGATTTCTTAAAAA ATCTAAGTTTAGAAGAACTACAGATGCGGCTAAAAGCACTAGACCCCATGATGGAACGAGAAATAGAAGA ACTGCATCAAAGATACAGTGCAAAAAGACAACCTATCCTGGATGCCATGGATGCGAAGAAGAGGAGGCAG CAGAATTTCTGACCAGATTCCCTCTGTCTCAGGATGACCAGAAACCAAGCAACCACAAGAATTGAAGGAA TTCTACTTTTTAATCCCTAAAATGTATATTCTACAATTTTTTAAAACTCAAAAATGATGGTACACACCTA GATAAATTTCCCAAAAGCAAACTTTACAGTTGTCTCCACTCTGATGTCGTTTTGAAAGGATGAGAACAAC AAACGTGCGCACTTCTCTCTCTGCCTGCTATTCCACCTGCAAGAGAACTAATCTAAAATGAAAGCTGTGG AGACTAGAAATCCAAAGCTGTTGCTTAGTCAATCATACAGCACTGGAGGGCTTTGTGTTCCAATGTCCTT TATTCCTATGGAGAAGCCTATTTATTGATCCCCCTCCCACCCCCCAGGTAGACTTATTTATTTATGCAGT TTCACTTTCTTGGGGTTTTTTTGTTTTTTTTAAGACCAGATCAGGATAGTTTTGGTGAATGTCCAGACAC ATTGCGGATTATAACACACAACCAAATTGTACACTAAGCAGGGAGCTCTGAGTGTACTCCTTCAACTCCA ATAGACACAAAGTCATGGCTGTAATGTTCAAATGGACCAGACATTTTATACTGAAGACTTAATACCATTT CTACCTTTTGAAGTGTTAATTCCTGATTTTGATATTGGTAAAAATAAAACTATATATACATAGGTCAAAA AGAGTACAGTGTAAAACCAAAGAAATCAATGAACTCTTGCACGAAAAAGTGTGGGAAATATTTTTAAAGA CATGAAACTGAGGGAAAAAAATGTAATTGTTAATGTTTCTTTTATTGGTCTGCAATAACTCATCAGGTTT ATCCTATGGGAATATGCTTCTGTCACAGGACTTTTTGTTGATTTCATTTATTTCCTGGGAATTGGTAAGC ATCATGTATCTGACAATAAAACAGTAAAAACAAAAACAAAAACTTTTATACTAAAAACTGTATCCTTAGG GCCTAAAAAAAAAAAAATTAAAGTATTAGAAAAAGACTCATTTCTGCAGAGCATTAGCATTTGTGTAATT GACCTGGGACCAGTCATTTCCTGCACTACACATGTCCTTTTTGACTGTGTGTGTGTGTTCTTTTATGTGT TAATTACTATGTGTGCCTCACAAAGACAAATAAATTCCATTGTGCCACCCAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_019635 |
| Insert Size | 1494 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC049123, AAH49123 |
| RefSeq Size | 2798 bp |
| RefSeq ORF | 1494 bp |
| Locus ID | 56274 |
| UniProt ID | Q9JI10 |
| Gene Summary | Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207107 | Stk3 (tGFP-tagged) - Mouse serine/threonine kinase 3 (Ste20, yeast homolog) (Stk3) |
CNY 4,850.00 |
|
| MR207107 | Stk3 (Myc-DDK-tagged) - Mouse serine/threonine kinase 3 (Ste20, yeast homolog) (Stk3) |
CNY 3,656.00 |
|
| MR207107L3 | Lenti ORF clone of Stk3 (Myc-DDK-tagged) - Mouse serine/threonine kinase 3 (Ste20, yeast homolog) (Stk3) |
CNY 6,270.00 |
|
| MR207107L4 | Lenti ORF clone of Stk3 (mGFP-tagged) - Mouse serine/threonine kinase 3 (Ste20, yeast homolog) (Stk3) |
CNY 6,270.00 |
