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Vil1 (NM_009509) Mouse Untagged Clone
CAT＃: MC201154
Vil1 (untagged) - Mouse villin 1 (Vil1), (10ug)
CNY 6,064.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Vil |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC015267 sequence for NM_009509
CCCAGGTGGTGGCTGCCTCTTCCAGACAGGCTCGTCCACCATGACTAAACTGAATGCCCAAGTCAAAGGC TCTCTCAACATCACCACTCCCGGGATACAGATATGGAGGATCGAGGCTATGCAGATGGTACCTGTTCCTT CCAGCACCTTTGGAAGCTTCTTCGATGGTGACTGCTATGTAGTCCTGGCTATCCACAAGACCAGCAGCAC TCTCTCCTATGATATCCACTACTGGATTGGCCAGGACTCGTCCCAGGATGAGCAGGGGGCAGCTGCCATC TACACCACACAGATGGATGACTACCTGAAGGGCCGGGCTGTCCAGCACCGCGAGGTTCAAGGCAACGAGA GCGAGACTTTCCGGAGCTACTTCAAGCAAGGCCTTGTGATCCGGAAAGGGGGAGTGGCTTCCGGCATGAA GCACGTAGAAACAAACTCCTGTGATGTCCAGCGACTGTTGCACGTCAAGGGCAAGAGGAATGTGCTGGCT GGAGAGGTGGAAATGTCCTGGAAGAGTTTCAACAGAGGGGATGTCTTCCTGCTGGACCTTGGGAAGCTTA TTATCCAGTGGAATGGGCCAGAGAGTAACCGCATGGAGAGACTTCGGGGCATGGCCTTGGCCAAAGAGAT CCGAGACCAGGAACGGGGTGGACGTACCTACGTAGGTGTGGTGGACGGGGAGAAGGAAGGGGACTCCCCA CAGCTGATGGCAATTATGAACCACGTGCTGGGCCCACGCAAAGAACTGAAGGCTGCTATTTCTGACTCAG TGGTGGAGCCGGCCGCTAAGGCTGCACTCAAGCTGTACCATGTGTCTGACTCCGAAGGAAAACTGGTGGT TAGAGAAGTTGCTACTCGGCCACTCACACAAGACCTGCTCAAGCATGAGGACTGTTACATCCTGGACCAG GGAGGCCTGAAGATCTTTGTGTGGAAGGGAAAAAATGCCAACGCTCAGGAGAGGAGCGGAGCCATGAGCC AGGCCTTGAACTTCATCAAAGCCAAGCAGTACCCACCGAGCACGCAGGTTGAGGTGCAGAATGACGGGGC CGAGTCCCCCATCTTCCAACAACTCTTCCAGAAGTGGACAGTGCCCAACCGGACCTCAGGCCTCGGCAAA ACCCACACTGTGGGCTCTGTGGCTAAGGTGGAACAGGTGAAGTTTGATGCTCTGACCATGCATGTACAAC CTCAGGTGGCTGCCCAGCAGAAAATGGTGGATGACGGGAGTGGGGAAGTGCAGGTGTGGCGCATCGAGGA CTTGGAGCTGGTGCCTGTGGAGTCCAAGTGGCTGGGCCATTTCTACGGTGGTGACTGCTACCTGCTGCTC TACACCTACCTCATAGGGGAAAAGCAACACTACTTGTTATATATCTGGCAGGGCAGCCAGGCCAGCCAGG ATGAAATTGCAGCCTCGGCGTATCAAGCCGTCCTGTTGGACCAGAAGTACAATGACGAGCCAGTACAGAT CCGGGTCACGATGGGCAAGGAGCCGCCTCACCTCATGTCTATCTTCAAGGGCCGCATGGTGGTTTATCAG GGAGGCACCTCCCGAAAGAACAACTTGGAGCCTGTGCCCTCTACGAGGCTATTTCAGGTCCGAGGGACCA ATGCTGATAACACCAAGGCTTTTGAGGTGACAGCCCGGGCCACGTCCCTCAACTCCAATGATGTCTTCAT ACTCAAGACTCCGTCCTGCTGCTACCTGTGGTGTGGGAAGGGCTGCAGTGGGGATGAGAGGGAGATGGCC AAGATGGTTGCTGATACCATCTCTCGGACGGAGAAACAAGTGGTAGTAGAGGGGCAGGAGCCAGCCAACT TCTGGATGGCTCTGGGCGGGAAGGCGCCCTACGCCAACACCAAGAGGCTGCAGGAGGAAAACCAAGTCAT CACTCCTCGGCTCTTCGAGTGCTCCAACCAGACCGGACGCTTTCTGGCCACAGAGATCTTTGACTTCAAT CAGGATGACCTGGAGGAGGAGGATGTGTTCCTATTGGATGTCTGGGACCAGGTCTTCTTCTGGATAGGGA AACATGCCAATGAGGAAGAGAAGAAGGCTGCAGCTACAACTGTACAAGAATACCTCAAGACCCACCCTGG AAACCGAGACCTTGAGACCCCTATCATCGTGGTGAAGCAGGGACACGAGCCCCCCACCTTCACAGGCTGG TTCCTGGCTTGGGATCCCTTCAAGTGGAGTAACACCAAATCCTATGATGACCTTAAGGCAGAGCTGGGAA ACTCTGGGGACTGGAGCCAGATTGCTGACGAGGTTATGAGCCCGAAAGTGGACGTTTTCACTGCCAATAC CAGTCTGAGTTCTGGGCCCCTGCCCACCTTCCCCCTGGAGCAGCTGGTAAACAAGTCTGTAGAGGATCTC CCTGAGGGTGTGGACCCCAGCAGGAAGGAGGAGCACCTGTCCACCGAAGACTTCACTAGGGCCTTGGGCA TGACTCCAGCTGCCTTCTCTGCCCTGCCTCGATGGAAGCAACAAAACATCAAGAAAGAAAAAGGACTGTT TTGAGAATTGAAGCTCTCTGGCTGTCCAGCAGCCCCTACCCTGCCTTCAAGGGCTTTGTGCCGCCATTAC TGGTTTTAGTCCTGTGGCAGATGAAAATGTCCAATTGTACCTGTGAGCCACAGTGTGACAATTCCTTTTG TTTATAATAGTAATTTGCCCATTCCTTCAGACGCATGCCACAGACCCATGGAAATCTTGTAGAGTTTTCT TTTCTTAGATGGACAGCTAAGTACTCCAGGAGACATTAGCGTCTGGGGGTTTCTCTGGCACCGTCACTCA CTCAGGATCTTATCCTGATCTTACCCTCCTCACACTCAAAAGGGAGGGCTAAGGCCAAAGCTGGGCTTAC AGCTCTAACCCAGAGCCTTTGCAAAGCTCCACAGACTCCTCAAATGACAACACCAGGAACATGGGTTTGC TACGTGAAGTCCAATCAGAAGCCAATAGGTGATTTTCTCTTAAAACTGGTTATCCAGTGTCCCCAGGAAC ATGTCCCTTTAAACAAATAAATCAAACTAATATGAGGTTAATAAAGGCTTTAATGTCTCTCACACATTAA AACAAAACAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_009509 |
| Insert Size | 2484 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC015267, AAH15267 |
| RefSeq Size | 3104 bp |
| RefSeq ORF | 2484 bp |
| Locus ID | 22349 |
| UniProt ID | Q62468 |
| Gene Summary | Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210849 | Vil1 (tGFP-tagged) - Mouse villin 1 (Vil1) |
CNY 7,980.00 |
|
| MR210849 | Vil1 (Myc-DDK-tagged) - Mouse villin 1 (Vil1) |
CNY 6,064.00 |
|
| MR210849L3 | Lenti ORF clone of Vil1 (Myc-DDK-tagged) - Mouse villin 1 (Vil1) |
CNY 9,120.00 |
|
| MR210849L4 | Lenti ORF clone of Vil1 (mGFP-tagged) - Mouse villin 1 (Vil1) |
CNY 9,120.00 |
