HLAE (HLA-E) Mouse Monoclonal Antibody [Clone ID: MEM-E/06]

Specifications

Product Data
Clone Name	MEM-E/06
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry. Immunoprecipitation. Immunohistochemistry on paraffin sections: 10 μg/ml; positive tissue: spleen.
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide
Specificity	The antibody recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
Formulation	Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	major histocompatibility complex, class I, E
Database Link	Entrez Gene 3133 Human UniProt ID P13747
Background	HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells.
Synonyms	HLA-6.2, HLAE, MHC class I antigen E
Reference Data

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