Plakophilin 3 (PKP3) Mouse Monoclonal Antibody [Clone ID: 12B11F8]

Specifications

Product Data
Clone Name	12B11F8
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunocytochemistry (methanol-fixed cells).  Immunohistochemistry on Frozen Tissues. Immunoprecipitation. Western blot detection. For optimal results, fixed cells or tissues should be treated with 0.2% Triton-X 100 for 5 min. prior to incubation with primary antibody. Recommended Dilutions: 1/25-1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 for Immunoblotting applications.
Reactivity	Canine, Human, Mouse, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to amino acid residues 779-793 of Human Plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CKLHRDFAKGYRKED).
Specificity	This antibody reacts with an epitope located between residues 779-793 in Human Plakophilin-3.
Formulation	PBS with 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	plakophilin 3
Database Link	Entrez Gene 11187 Human UniProt ID Q9Y446
Background	Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.
Synonyms	Plakophilin-3
Reference Data

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