NF-kB p65 (RELA) pSer468 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, IP, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200. Immunoprecipitation: 1/50-1/200.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of NFκB-p65 pSer468 protein.
Formulation	Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 60 kDa
Gene Name	RELA proto-oncogene, NF-kB subunit
Database Link	Entrez Gene 5970 Human UniProt ID Q04206
Background	Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp kB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and rapidly transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct 50 kDa proteins have been described, designated p105 and p100, respectively. The p105 precursor contains p50 at its amino terminus and a C-terminal region that when expressed as a separate molecule, designated PDI, binds to p50 and regulates its activity.
Synonyms	NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3
Reference Data

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