Supt5h (NM_001107497) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217463 representing NM_001107497 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACAGCGAAGACAGCAACTTCTCGGAGGAGGAGGACAGCGAGCGCAGCAGCGAGGCGGAGGAGG CCGAGGTAGAGGAAGACCGGCGAAGTGCCGCAGGCAGTGAAAAAGAAGAGGAGCCCGAGGAAGAGGAGGA GGAGGAAGAGGAGGAATACGATGAGGAGGAGGAGGAGGAGGATGATGACCGGCCTCCCAAGAAACCACGC CATGGGGGCTTCATTTTAGATGAGGCTGATGTCGATGATGAATATGAAGATGAAGACCAGTGGGAAGATG GAGCTGAAGATATCCTGGAGAAAGAAGAGATTGAAGCCTCCAACATTGATAACGTTGTGCTGGATGAAGA CCGCTCTGGGGCTCGTCGTCTGCAAAATCTCTGGAGGGACCAGAGAGAAGAAGAACTGGGCGAGTATTAC ATGAAGAAATATGCCAAGTCATCTGTGGGAGAGACGGTATATGGAGGGTCTGATGAGCTCTCAGATGACA TCACCCAGCAGCAGCTGCTCCCAGGAGTCAAGGACCCCAACCTGTGGACGGTCAAGTGTAAGATTGGGGA GGAACGGGCCACAGCAATTTCCTTGATGCGAAAATTCATCGCTTACCAGTTCACAGACACACCCCTGCAG ATTAAGTCAGTGGTTGCCCCGGAGCATGTGAAGGGCTACATCTATGTGGAGGCCTACAAACAGACCCATG TGAAGCAGGCCATTGAGGGTGTGGGCAACCTGCGGCTTGGCTACTGGAACCAGCAGATGGTGCCTATTAA GGAGATGACCGATGTACTCAAGGTGGTGAAGGAGGTGGCCAACCTGAAGCCGAAGTCCTGGGTCCGCCTC AAGAGAGGCATCTACAAAGATGACATCGCTCAGGTGGACTACGTCGAGCCCAGCCAGAACACCATTTCCC TGAAGATGATCCCGCGCATTGACTATGACCGCATCAAAGCCCGCATGAGCTTGAAAGACTGGTTTGCCAA AAGGAAGAAGTTCAAGCGGCCTCCACAGAGGCTCTTTGATGCTGAAAAGATCAGGTCCTTGGGGGGTGAT GTTGCCTCTGATGGTGACTTCCTCATCTTTGAGGGTAACCGCTACAGCCGGAAGGGCTTCCTGTTCAAGA GTTTTGCCATGTCTGCTGTGATTACAGAAGGTGTAAAGCCCACCCTGTCAGAGCTGGAAAAGTTTGAGGA TCAGCCTGAGGGCATCGACTTGGAGGTGGTGACTGAAAGCACAGGGAAGGAGCGAGAGCACAATTTCCAG CCTGGTGACAATGTGGAGGTGTGTGAGGGTGAGCTCATCAACCTGCAGGGCAAGGTCCTCAGCGTGGATG GCAACAAGATCACCATCATGCCCAAACATGAGGACCTCAAGGACATGCTTGAGTTCCCAGCTCAGGAACT TCGGAAGTACTTCAAGATGGGAGACCATGTAAAAGTGATTGCTGGCCGATTTGAGGGTGACACAGGTCTC ATCGTGCGGGTGGAAGAGAACTTTGTTATTCTTTTCTCAGACCTCACCATGCACGAGTTGAAAGTGCTTC CCCGGGATCTGCAGCTCTGCTCAGAGACAGCCTCAGGTGTGGATGTTGGGGGCCAGCATGAGTGGGGTGA GCTGGTGCAGTTGGATCCCCGGACTGTAGGTGTCATCGTGCGGCTGGAGCGAGAGACCTTCCAGGTTCTC AACATGCATGGGAAAGTGGTGACTGTAAGGCACCAGGCTGTGACGCAGAAAAAGGACAACCGCTTCGCTG TGGCTCTGGACTCAGATCAGAACAACATCCATGTGAAGGACATTGTTAAGGTCATTGATGGCCCCCATTC AGGCCGAGAGGGTGAGATCCGCCATCTGTACCGCAGCTTTGCCTTCCTGCATTGCAAGAAGCTGGTGGAG AATGGCGGCATGTTTGTCTGTAAGGCACGGCACCTGGTGTTGGCTGGGGGCTCAAAGCCCCGGGATGTGA CCAATTTAACTGTTGGTGGCTTCACTCCTATGAGCCCCCGGATCAGCAGCCCTATGCACCCCAGTGCTGA AGGTCAGCATGGTGGTTTTGGAAGCCCAGGTGGCATGAGCAGGGGTCGAGGGCGAAGAGACAATGAGCTC ATAGGCCAGACTGTGCGTATCTCCCAGGGACCCTACAAAGGCTACATTGGTGTGGTGAAGGATGCCACGG AGTCCACAGCCCGAGTAGAATTGCACTCTACCTGCCAGACCATCTCTGTGGACCGCCAGCGGCTCACCAC AGTTGACTCCCAGCGTCCAGGTGGCATGACTTCCACCTATGGAAGGACTCCCATGTATGGCTCTCAGACT CCCATGTATGGCTCTGGCTCCCGAACACCCATGTATGGCTCCCAGACACCTCTGCAGGATGGTAGCCGCA CTCCACATTATGGCTCACAGACACCCCTGCATGATGGTAGCCGCACTCCTGCTCAGAGTGGGGCCTGGGA TCCCAACAACCCTAACACACCTTCCAGGGCTGAGGAGGAATACGAATATGCCTTTGACGACGAACCCACC CCATCCCCACAAGCATATGGGGGAACCCCGAATCCCCAAACACCCGGCTACCCAGACCCATCATCCCCCC AGGTCAATCCACAGTACAACCCGCAGACACCCGGGACGCCAGCCATGTACAACACAGACCAGTTCTCCCC CTACGCTGCCCCCTCCCCACAGGGGTCTTACCAGCCCAGCCCCAGCCCCCAGAGCTACCACCAAGTGGCA CCAAGTCCAGCGGGCTATCAGAACACCCATTCCCCAGCCAGCTACCACCCAACACCGTCACCCATGGCCT ATCAGGCTAGTCCCAGCCCCAGCCCTGTTGGCTACAGTCCAATGACCCCTGGAGCTCCTTCTCCTGGAGG CTACAATCCCCACACACCAGGCTCAGGCATTGAGCAGAACTCCAGTGACTGGGTGACCACTGATATCCAG GTGAAGGTTCGAGACACCTATCTGGACACACAAATAGTGGGGCAGACAGGCGTCATCCGCAGTGTCACGG GAGGCATGTGCTCCGTGTACCTGAAAGATAGTGAGAAGGTGGTCAGTATCTCCAGTGAGCATCTGGAGCC CATCACCCCCACCAAGAACAACAAGGTGAAAGTGATTCTGGGTGAGGACCGAGAGGCTACCGGGGTCCTT CTGAGTATTGATGGTGAGGATGGCATCATCCGAATGGACCTGGAAGACCAGCAGATCAAGATTCTAAACC TCCGCTTCCTAGGGAAGCTCCTGGAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217463 representing NM_001107497 Red=Cloning site Green=Tags(s) MSDSEDSNFSEEEDSERSSEAEEAEVEEDRRSAAGSEKEEEPEEEEEEEEEEYDEEEEEEDDDRPPKKPR HGGFILDEADVDDEYEDEDQWEDGAEDILEKEEIEASNIDNVVLDEDRSGARRLQNLWRDQREEELGEYY MKKYAKSSVGETVYGGSDELSDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFIAYQFTDTPLQ IKSVVAPEHVKGYIYVEAYKQTHVKQAIEGVGNLRLGYWNQQMVPIKEMTDVLKVVKEVANLKPKSWVRL KRGIYKDDIAQVDYVEPSQNTISLKMIPRIDYDRIKARMSLKDWFAKRKKFKRPPQRLFDAEKIRSLGGD VASDGDFLIFEGNRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVVTESTGKEREHNFQ PGDNVEVCEGELINLQGKVLSVDGNKITIMPKHEDLKDMLEFPAQELRKYFKMGDHVKVIAGRFEGDTGL IVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDVGGQHEWGELVQLDPRTVGVIVRLERETFQVL NMHGKVVTVRHQAVTQKKDNRFAVALDSDQNNIHVKDIVKVIDGPHSGREGEIRHLYRSFAFLHCKKLVE NGGMFVCKARHLVLAGGSKPRDVTNLTVGGFTPMSPRISSPMHPSAEGQHGGFGSPGGMSRGRGRRDNEL IGQTVRISQGPYKGYIGVVKDATESTARVELHSTCQTISVDRQRLTTVDSQRPGGMTSTYGRTPMYGSQT PMYGSGSRTPMYGSQTPLQDGSRTPHYGSQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAFDDEPT PSPQAYGGTPNPQTPGYPDPSSPQVNPQYNPQTPGTPAMYNTDQFSPYAAPSPQGSYQPSPSPQSYHQVA PSPAGYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSGIEQNSSDWVTTDIQ VKVRDTYLDTQIVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITPTKNNKVKVILGEDREATGVL LSIDGEDGIIRMDLEDQQIKILNLRFLGKLLEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107497
ORF Size	3249 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107497.2, NP_001100967.2
RefSeq Size	3568 bp
RefSeq ORF	3252 bp
Locus ID	308472
MW	120.8 kDa

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