Ciita (NM_001270804) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C2ta; Mhc2ta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217439 representing NM_001270804 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGGGGTCTCCAGAGGGCAGCTACCTGGAGCTCCTTAACAGTGATGCTGACCCCCTACACCTCT ACCACCTCTATGACCAGATGGACCTTGCTGGGGAGGAAGAGATCGAACTCAACTCAGAGACAGACATGGA CACCATCAACTGTGACCAGTTCAGCAAGCTGTTGCAGGATATGGAAATGGATGAAGAGACCCGGGAGGCC TATGCCAATATCGCGGAACTGGATCAGTACGTGTTCCAGGATACCCAGCTGGAGGGCCTGAGCAAGGACC TCTTCATACAGCACATTGGACCAGAGGAAGGCTTTGGTGAGAACGTAGAGGGCCCTGCAGAATCAGGAGA GAAGCCTCAGAAGAGACGCTTCCCAGAAGAGCATGCTATGGACTCAAAGCACAGGAAACTAGTGCCTCCC TCTAGGACCTCACTGAACTATTTGGATCTCCCCGCTGGGCATATCCAGATCTTCACCACTCTGCCCCAGG GACTCTGGCAAATCTCAGGGGTTGGCACAGGGCTCTCCAGTGTCCTAGTCTACCACGGTGAGATGCCCCA GGTCAACCAATTACTCTCTTCAAGCAGCCCCACTGTCCCCAGTCTCCCCGAGTCCCCAGACCGGCCTGGC TCCACCAGCCCCTTCGCGCCATCCACAGCTGACCTGCCCGGCATGCCTGAACCTGCTCTGACCTCCCGTG TAAATGAGACAGAGGACACAACACCCCCCTCCCCATGCCAAGAGGATCCCGAGCCTTCCATCAAGCTTCC AAAATGGCCAGAGTCCGTGGAGCGGTTCCAGCACTCCCTACAGGACAAGTACCAAGCACTGCCCGGGAGC CCAAGTGGTCCTCTGGTAGCCATGGAGCTGGTGCGGGCCAGGTTGGAAAGAGGTAGCAACAAGAGTCAGG AAAGGGAGCTGGCCACTCCTGACTGGACAGAGCGCCAGCTAGCCCATGGCGGTCTGGCCGAGGTACTTCG GGTTGTCGGCGACTCCAGGAGATCACGAGAGACACAGGTGGTCGCTGTGCTGGGCAAGGCTGGTCAGGGC AAGAGCCACTGGGCCAGGACAGTGAGCCACATTTGGGCACAAGGCCAGTTACCACAATATGACTTTGTCT TCTACGTCCCCTGTCAATGCTTGGATCGCCCTGGGGACACCTACCACCTGCGGGATCTGCTCTGTCCCCC GAACCTGCAGCCACTGGCCATGGATGACGAGGTCATTGATCATATTGTGAGGCAGCCGGACCGTGTTCTG CTCATCCTAGATGCTTTTGAGGAGCTAGAGGCCCAAGACAGCCTCCTGCATGGACCCTGTGGACCTCTGT CCCCAGAGCCCTGCTCCCTCCGGGGACTGCTGGCTGGGCTCTTCCAGCGTAAGCTGCTGCGAGGCTGCAC CCTGCTCCTCACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCAAGGCAGATGCCATCTTCGAG GTGCCCAGCTTCTCTACGAAACAGGCCCAGATCTATATGAGGCACTACTTTGAGAACTCAGGGACAGCAG GGAGTCACGACAAGGCCCTGGGCCTCCTGGAGGGCCAGCCTTTTCTCCTCACCCACAGTCACAGCCCTGT TCTGTGCAGTGCCGTGTGTCAGCTCTCCAAGGCCCTGCTGGATCAGGGCGCAGAGGCCCAGCTACCTTGC ACACTCACAGGACTCTATGTTGGCCTGCTAGGCCCTGCAGCTCAGAACAGTCCTCCTGGAGCCTTAGTTG AGTTGGCCAAGCTAGCCTGGGAGCTGGGACGGAGGCACCAAAGCACCTTGCAGGAAACCCAGTTTCCATC CGTGGAAGTGAAGACCTGGGCTGTGGCCCAAGGCTTGATGCAGCAGACCACAGAGGCCCAACTGGCTTTC TCCAGTTTTCTGCTACAGTGCTTCCTGGGTGCTGTGTGGCTGGCACAGTGCAATGAGATGAAAGACAAGG AGCTGCCACAGTACCTGGCCTTGACCCCGAGGAAGAAGCGACCCTATGACAACTGGCTGGAGGGCGTACC ACGCTTTCTGGCTGGCTTAGTTTTCCAGCCTCGAGCCCACTGCCTGGGAGCTCTGGTGGAGCCTGCAATG GCTGCAGTGGCAGATAGGAAACGGAAGGTTCTTACCAGGTACCTGAAGCGCTTGAAGCTGGGGACACTTC GGGCCGGGAGGTTGCTGGAGCTGCTACACTGTGCCCACGAAACACAGGAACCTGGTATTTGGAAGCATGT TTCACACCAGCTCCCAGGGCACCTCTCCTTCCTGGGCACACGGCTCACAGCCCCAGATGTGTATGTTCTG GGCAGGGCCTTGGAGACAGCCAGCCAGGACTTCTCCTTGGACCTTCGTCAGACTGGCATCGAGCCCTCCA GACTGGGAGACCTCGTGGGACTCAGCTGTGTCACCAGTTTCAGGGCCTCCTTGAGTGACACAATGGCATT ATGGGAGTCCCTACAGCATCAGGGAGAAACCCAGCTACTCCAGGCGGCAGAGGAGAAGTTCACCATTGAG CCATTTAAGGCCAAATCCCCAAAGGATGTGGAAGACCTGGACAGCCTTGTGCAGACCCAGAGGCTGAGAA ACCCCTCAGCGGATGCAGCCAAGGATCTTCCTGCCATCCGGGACCTTAAGAAGCTAGAGTTTGCATTGGG CCCCGTCTTGGGCCCGCAGGCTTTCCCCACACTGGCCAAGATCCTTCCAGCATTCTCTTCCCTGCAGCAC CTGGACCTGGACTCACTTAGCGAGAACAAGATCGGGGACAAGGGTGTGTCGAAGCTCTCAGCCACCTTCC CTCAGCTGAAGGCCCTGGAGACGCTCAACCTGTCCCAGAATAGCATCACCGATGTGGGAGCTTGCAAGCT TGCAGAAGCCCTGCCGGCCCTGGCCAAGTCCCTCCTAAGGCTGAGCTTGTACAACAACTGCATCTGTGAC GAAGGGGCCAAGAGCCTGGCACGAGTGCTTCCAGACATGGTGTCCCTCCGGGTGATGGATGTCCAGTTCA ACAAGTTCACGGCTGTCGGGGCCCAGCAGCTGACCTCCAGCCTTCAGAAGTGCCCTCAGGTGGAGACACT GGCAATGTGGACACCCACTATCCCCTTTGGGGTCCAGGAACACCTGCAGCAGCTGGATGCCCGGATTAGC CTGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217439 representing NM_001270804 Red=Cloning site Green=Tags(s) MELGSPEGSYLELLNSDADPLHLYHLYDQMDLAGEEEIELNSETDMDTINCDQFSKLLQDMEMDEETREA YANIAELDQYVFQDTQLEGLSKDLFIQHIGPEEGFGENVEGPAESGEKPQKRRFPEEHAMDSKHRKLVPP SRTSLNYLDLPAGHIQIFTTLPQGLWQISGVGTGLSSVLVYHGEMPQVNQLLSSSSPTVPSLPESPDRPG STSPFAPSTADLPGMPEPALTSRVNETEDTTPPSPCQEDPEPSIKLPKWPESVERFQHSLQDKYQALPGS PSGPLVAMELVRARLERGSNKSQERELATPDWTERQLAHGGLAEVLRVVGDSRRSRETQVVAVLGKAGQG KSHWARTVSHIWAQGQLPQYDFVFYVPCQCLDRPGDTYHLRDLLCPPNLQPLAMDDEVIDHIVRQPDRVL LILDAFEELEAQDSLLHGPCGPLSPEPCSLRGLLAGLFQRKLLRGCTLLLTARPRGRLAQSLSKADAIFE VPSFSTKQAQIYMRHYFENSGTAGSHDKALGLLEGQPFLLTHSHSPVLCSAVCQLSKALLDQGAEAQLPC TLTGLYVGLLGPAAQNSPPGALVELAKLAWELGRRHQSTLQETQFPSVEVKTWAVAQGLMQQTTEAQLAF SSFLLQCFLGAVWLAQCNEMKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLVFQPRAHCLGALVEPAM AAVADRKRKVLTRYLKRLKLGTLRAGRLLELLHCAHETQEPGIWKHVSHQLPGHLSFLGTRLTAPDVYVL GRALETASQDFSLDLRQTGIEPSRLGDLVGLSCVTSFRASLSDTMALWESLQHQGETQLLQAAEEKFTIE PFKAKSPKDVEDLDSLVQTQRLRNPSADAAKDLPAIRDLKKLEFALGPVLGPQAFPTLAKILPAFSSLQH LDLDSLSENKIGDKGVSKLSATFPQLKALETLNLSQNSITDVGACKLAEALPALAKSLLRLSLYNNCICD EGAKSLARVLPDMVSLRVMDVQFNKFTAVGAQQLTSSLQKCPQVETLAMWTPTIPFGVQEHLQQLDARIS LR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270804
ORF Size	3156 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270804.1, NP_001257733.1
RefSeq Size	5004 bp
RefSeq ORF	3159 bp
Locus ID	85483
MW	116.1 kDa
Gene Summary	may play a role in transcriptional activation of MHC class II genes [RGD, Feb 2006]

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