Ube3a (NM_001191837) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217280 representing NM_001191837 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACAGCTTGTAAAAGATCATCAGGAGAATCCCAGTCTGAGGACATTGAAGCTAGCCGAATGAAGC GAGCAGCTGCAAAGCATCTAATAGAGCGCTACTACCATCAGTTAACTGAGGGCTGTGGAAATGAGGCCTG CACGAATGAGTTCTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAACAATGCAGCAGCTATTAAAGCT CTTGAGCTTTATAAAATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTT ACCTTGAGAACTCAAAAGGTGCATCTAACAACTCAGAGATAAAAATGAACAAGAAGGAAGAGTTTAAAGA GGTGGACTACCTAACTGAAGAGAAAGTATATGAAATTTATGAATTTTGTAGGGAGAGAGAGGATTACTCC CCTTTAATTCGTGTAATTGGAAGAATATTTTCTAGTGCTGAGGCACTGGTTCAGAGCTTTCGGAAAGTCA AACAGCACACTAAGGAGGAATTGAAATCTCTTCAAGAGAAGGATGAAGACAAGGATGAAGATGAAAAGGA AAAAGCTGCGTGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCTTCAAGGATGGGTGATAGC TCACAGGGAGACAACAATGTACAAAAATTAGGTCCTGATGATGTGACTGTGGATATTGATGCTATAAGAA GGGTCTACAGCAGTTTGCTCTCTGATGAAAAAATAGAAACTGCCTTCCTGAATGCACTTGTATATCTGTC ACCTAACGTGGAATGTGATTTGACATATCATAATGTGTACACTCGAGACCCTAATTATCTCAATTTGTTC ATTATTGTAATGGAAAATAGGAATCTCCACAGTCCTGAATATCTGGAAATGGCATTGCCGTTATTTTGCA AAGCTATGAGTAAGCTACCCCTTGCAGCTCAAGGAAAACTGATTAGGCTGTGGTCTAAATACAGTGCTGA CCAGATTCGGAGAATGATGGAAACATTTCAGCAACTTATTACCTACAAAGTCATAAGCAATGAATTTAAT AGCCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCAAAGTGTTTGAAAATGGTTTACTATG CAAATGTAGTGGGAGGGGACGTGGACACAAATCATAATGAGGAAGATGATGAAGAGCCTATACCTGAGTC CAGTGAATTGACACTTCAGGAGCTTCTGGGAGAGGAAAGAAGAAATAAGAAAGGTCCTCGAGTGGATCCA CTAGAAACCGAAATTGGCGTTAAAACTCTAGATTGTCGAAAACCACTTATCTCCTTTGAAGAATTCATTA ATGAACCACTGAATGATGTTCTAGAAATGGACAAAGATTATACTTTTTTCAAAGTTGAAACAGAGAACAA ATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATCTGGGATTGTATTATGACAAT AGAATTCGCATGTACAGTGAAAGAAGAATCACTGTTCTTTACAGCCTAGTTCAAGGACAGCAGTTGAATC CATATTTGAGACTTAAAGTCAGACGTGACCATATTATAGATGATGCACTTGTCCGGCTAGAGATGATCGC TATGGAAAATCCTGCAGACTTGAAGAAACAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGTAGATGAG GGAGGCGTTTCCAAAGAGTTTTTTCAGTTGGTTGTGGAGGAAATCTTTAATCCAGATATTGGTATGTTCA CATACGATGAAGCTACAAGATTATTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAATTTACTCT GATTGGCATAGTCCTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTCCATTTTCCCATGGTTGTG TACAGGAAGCTAATGGGGAAAAAAGGAACGTTTTGTGACTTGGGAGACTCTCACCCAATCTTATATCAGA GTTTAAAGGATTTATTGGAATATGAAGGGAATGTGGAAGATGATATGATGATCACTTTCCAGATATCACA GACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAAGAAAATGGTGATAAAATTCCAATTACAAAT GAAAACAGGAAGGAATTTGTCAGTCTCTATTCAGACTACATTCTCAATAAATCTGTAGAAAAACAATTCA AGGCATTTCGCAGAGGTTTTCATATGGTGACTAATGAATCACCCTTAAAATACTTATTCAGACCAGAAGA AATTGAATTACTTATATGTGGAAGCCGGAATCTCGATTTCCAGGCACTAGAAGAAACTACAGAGTATGAT GGTGGCTATACGAGAGAATCTGTTGTGATTAGGGAGTTCTGGGAAATTGTTCATTCGTTTACAGATGAAC AGAAAAGACTTTTCCTGCAGTTTACAACAGGCACAGACAGAGCACCTGTTGGAGGACTCGGAAAATTGAA GATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTGCTTTAATGTCCTT TTACTTCCGGAATATTCAAGCAAAGAAAAACTTAAAGAGAGATTGCTGAAGGCCATCACATATGCCAAAG GATTTGGCATGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217280 representing NM_001191837 Red=Cloning site Green=Tags(s) MATACKRSSGESQSEDIEASRMKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKA LELYKINAKLCDPHPSKKGASSAYLENSKGASNNSEIKMNKKEEFKEVDYLTEEKVYEIYEFCREREDYS PLIRVIGRIFSSAEALVQSFRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDS SQGDNNVQKLGPDDVTVDIDAIRRVYSSLLSDEKIETAFLNALVYLSPNVECDLTYHNVYTRDPNYLNLF IIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNEFN SRNLVNDDDAIVAASKCLKMVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDP LETEIGVKTLDCRKPLISFEEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDN RIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDE GGVSKEFFQLVVEEIFNPDIGMFTYDEATRLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVV YRKLMGKKGTFCDLGDSHPILYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITN ENRKEFVSLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYD GGYTRESVVIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVL LLPEYSSKEKLKERLLKAITYAKGFGML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191837
ORF Size	2604 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191837.2, NP_001178766.1
RefSeq Size	5795 bp
RefSeq ORF	2607 bp
Locus ID	361585
MW	99.8 kDa

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