Hsf1 (NM_024393) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216847 representing NM_024393 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTGGCTGTGGGCCCCGGTGCAGCGGGGCCCAGCAACGTCCCGGCCTTCCTAACCAAGCTGTGGA CCCTCGTGAGCGACCCGGACACAGACGCGCTCATCTGCTGGAGCCCGAGTGGGAACAGCTTCCACGTGTT TGACCAGGGCCAGTTTGCCAAGGAGGTGCTGCCCAAGTACTTCAAGCACAACAACATGGCCAGCTTTGTG CGGCAGCTCAACATGTATGGCTTCCGAAAAGTAGTCCACATTGAGCAGGGTGGCCTGGTCAAGCCTGAGA GGGATGACACCGAGTTCCAGCATCCATGTTTCCTGCGTGGCCAGGAACAGCTCCTTGAGAATATCAAGAG GAAAGTGACCAGCGTGTCCACCCTGAAGAGTGAGGACATAAAAATACGCCAGGACAGTGTCACCAGGCTG TTGACAGATGTGCAGCTGATGAAGGGGAAACAGGAGTGTATGGACTCCAAGCTCCTGGCCATGAAGCACG AGAACGAGGCCCTGTGGCGGGAGGTGGCCAGCCTTCGGCAGAAGCATGCCCAGCAGCAAAAAGTTGTCAA CAAGCTCATCCAATTCCTGATCTCACTGGTGCAGTCGAACCGGATCCTGGGGGTGAAGAGAAAGATCCCT CTGATGTTGAGTGACAGCAGCTCAGCACACTCTGTGCCCAAGTATGGTCGACAGTACTCCCTGGAGCATG TCCATGGTCCCGGCCCATACTCAGCTCCATCTCCAGCCTACAGCAGCTCTAGCCTTTACTCCTCTGATGC TGTCACCAGCTCTGGGCCCATAATCTCCGATATCACTGAGCTGGCTCCCACCAGCCCTTTGGCCTCCCCA GGCAGGAGCATAGATGAGAGGCCTCTGTCCAGCAGCACCCTGGTCCGTGTCAAGGAAGAGCCCCCCAGCC CACCTCATAGCCCTCGGGTACTGGAGGCAAGCCCTGGGCGCCCAGCCTCCATGGATACCCCTTTGTCCCC AACTGCCTTCATTGACTCCATCCTTCGAGAGAGCGAACCTACCCCTGCTGCCTCAAACACAGCCCCTATG GACACAACCGGAGCCCAAGCCCCTGCACCCCCAGCCCCCTCCACCCCTGAGAAGTGCCTCAGCGTAGCCT GCCTAGACAAGAACGAGCTAAGTGATCACCTGGATGCCATGGACTCCAACCTGGACAACCTGCAGACCAT GCTGACAAGCCACGGCTTCAGTGTGGACACCAGTGCCCTGCTGGACCTGTTCAGCCCCTCGGTGACCATG CCCGACATGAGCCTGCCTGACCTGGACAGCAGCCTGGCCAGCATTCAGGAACTTCTATCTCCCCAAGAGC CTCCCAGGCCTATTGAGGCAGAGAATAGTAACGCCGACTCAGGAAAGCAGCTGGTGCACTACACGGCTCA GCCTCTGTTCCTGCTGGACCCTGATGCTGTGGACACAGGGAGCAGTGAACTGCCTGTGCTCTTTGAGCTG GGGGAGAGCTCCTACTTCTCTGAGGGAGATGACTACACGGATGATCCCACCATCTCTCTTCTGACGGGCA CTGAACCCCACAAAGCCAAGGACCCCACTGTCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216847 representing NM_024393 Red=Cloning site Green=Tags(s) MDLAVGPGAAGPSNVPAFLTKLWTLVSDPDTDALICWSPSGNSFHVFDQGQFAKEVLPKYFKHNNMASFV RQLNMYGFRKVVHIEQGGLVKPERDDTEFQHPCFLRGQEQLLENIKRKVTSVSTLKSEDIKIRQDSVTRL LTDVQLMKGKQECMDSKLLAMKHENEALWREVASLRQKHAQQQKVVNKLIQFLISLVQSNRILGVKRKIP LMLSDSSSAHSVPKYGRQYSLEHVHGPGPYSAPSPAYSSSSLYSSDAVTSSGPIISDITELAPTSPLASP GRSIDERPLSSSTLVRVKEEPPSPPHSPRVLEASPGRPASMDTPLSPTAFIDSILRESEPTPAASNTAPM DTTGAQAPAPPAPSTPEKCLSVACLDKNELSDHLDAMDSNLDNLQTMLTSHGFSVDTSALLDLFSPSVTM PDMSLPDLDSSLASIQELLSPQEPPRPIEAENSNADSGKQLVHYTAQPLFLLDPDAVDTGSSELPVLFEL GESSYFSEGDDYTDDPTISLLTGTEPHKAKDPTVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_024393
ORF Size	1575 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_024393.1, NP_077369.1
RefSeq Size	2415 bp
RefSeq ORF	1578 bp
Locus ID	79245
MW	57.1 kDa
Gene Summary	involved in regulating the transcription of the heat shock proteins involved in the cochlear stress response [RGD, Feb 2006]

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