Pou4f2 (NM_134355) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Brn-3.2; Brn-3B; Brn3b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216599 representing NM_134355 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGATGATGTCCCTGAATAGCAAGCAGGCGTTCAGCATGCCTCATGCCGGCAGCCTGCATGTGGAGC CCAAGTACTCGGCGCTGCACAGTGCCTCCCCGGGTTCCTCTGCGCCCGCGGCGCCCTCGGCCAGTTCCCC TAGCAGCTCCAGCAACGCTGGCAGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGTGGTGGCGGAGGCCGG AGCAGCAGTTCCAGCAGCAGTGGCAGCGGCGGCGGCGGCGGCGGGGGCTCGGAGGCGATGCGGAGAGCTT GTCTTCCAACCCCACCGAGCAATATATTCGGCGGTCTGGATGAGAGTCTGCTGGCCCGTGCCGAGGCTCT GGCCGCCGTGGACATCGTCTCCCAGAGTAAGAGCCACCACCACCATCCGCCCCACCACAGCCCCTTCAAG CCGGACGCCACTTACCACACCATGAACACCATCCCGTGCACGTCTGCCGCTTCCTCTTCATCCGTGCCCA TCTCGCACCCGTCCGCTCTGGCTGGCACCCACCACCATCACCACCACCACCATCACCACCACCACCAGCC ACATCAGGCTCTGGAGGGCGAGCTGCTGGAGCACCTGAGCCCCGGGCTGGCCCTGGGAGCCATGGCGGGC CCCGACGGCACGGTGGTGTCCACTCCGGCTCACGCACCGCACATGGCCACCATGAACCCCATGCACCAAG CAGCCCTGAGCATGGCCCACGCGCACGGTCTGCCCTCGCACATGGGCTGCATGAGCGACGTGGATGCAGA CCCGCGGGACCTGGAGGCGTTCGCCGAGCGCTTCAAGCAGCGACGCATCAAGCTGGGAGTGACCCAGGCG GATGTGGGTTCGGCGCTGGCCAACCTCAAGATACCGGGCGTAGGCTCGCTCAGCCAGAGCACCATCTGCA GGTTTGAGTCCCTCACGCTGTCGCACAACAACATGATCGCGCTCAAGCCCATCCTGCAGGCGTGGCTGGA GGAAGCAGAGAAATCCCACCGCGAGAAGCTCACCAAGCCCGAGCTCTTCAATGGCGCGGAGAAGAAGCGC AAGCGCACGTCCATCGCGGCGCCCGAGAAGCGCTCACTGGAAGCCTACTTCGCCATCCAGCCAAGGCCCT CCTCGGAGAAGATCGCGGCCATCGCCGAGAAGCTGGATCTCAAGAAAAACGTAGTGCGCGTCTGGTTCTG CAACCAGAGGCAGAAACAGAAGAGAATGAAATACTCAGCCGGCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216599 representing NM_134355 Red=Cloning site Green=Tags(s) MMMMSLNSKQAFSMPHAGSLHVEPKYSALHSASPGSSAPAAPSASSPSSSSNAGSGGGGGGGGGGGGGGR SSSSSSSGSGGGGGGGSEAMRRACLPTPPSNIFGGLDESLLARAEALAAVDIVSQSKSHHHHPPHHSPFK PDATYHTMNTIPCTSAASSSSVPISHPSALAGTHHHHHHHHHHHHQPHQALEGELLEHLSPGLALGAMAG PDGTVVSTPAHAPHMATMNPMHQAALSMAHAHGLPSHMGCMSDVDADPRDLEAFAERFKQRRIKLGVTQA DVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGAEKKR KRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAGI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_134355
ORF Size	1236 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_134355.1, NP_599182.1
RefSeq Size	3210 bp
RefSeq ORF	1239 bp
Locus ID	171355
UniProt ID	G3V7L5
MW	43.2 kDa
Gene Summary	member of the POU family of transcription factors; play key roles in the development of specific neuronal cell types [RGD, Feb 2006]

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