Plpp6 (NM_001034854) Rat Tagged ORF Clone

CAT#: RR214851

  • TrueORF®

Ppapdc2 (Myc-DDK-tagged ORF) - Rat phosphatidic acid phosphatase type 2 domain containing 2 (Ppapdc2), (10 ug)



  "NM_001034854" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Cited in 1 publication.

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Ppapdc2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR214851 representing NM_001034854
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGAGCCCCCGGAGGACTATCGAGGGACGACCGCTAGGCTCCTCGGGCGGAAGCAGCGTCCCGGGCA
GCCCGGCCCACGGCGGCGGCGGCGGCGGCAGCGGCAGGTTCGAGTTCCAGTCGCTGCTGAGCTGCCGCTC
GGGCGCGGACCCCGCCTGCGCCCGGCTGCGCGCGTCCGACAGCCCGGTGCATCGGCGCGGCTCCTTCCCG
CTGGCTGCCGCGTGCCCTGCACAAGTAGCTCCTGCCCCACCGCCGGAGGACGCCGGCATGAACTTGAACC
CGTCCTTCCTGGGCATCGCCCTGCGCTCCCTGCTGGCCATCGACCTGTGGCTGTCCAAGAAGCTGGGGGT
GTGTGCTGGGGAGAGCTCGGCCTGGGGCAGCGTGCGGCCCCTGATGAAGCTGCTGGAGATCTCAGGACAT
GGCATCCCCTGGTTGCTGGGCACCCTCTACTGCCTGCTTAGGAGCGACAGCTGGGCCGGGCGCGAAGTGC
TGATGAACCTGCTCTTCGCCCTGCTGTTGGACCTACTGCTGGTGGCTGTGATTAAAGGGTTGGTCCGCAG
GCGCCGACCGGCGCACAATCAGATGGACATGTTTTTCACCCTCTCGGTGGACAAGTACTCCTTTCCCTCG
GGTCATGCCACGAGGGCCGCCCTGGTGTCGCGGTTCATCCTGAACCACCTGGTACTGGCCATTCCACTGA
GGGTGCTGGTGGTCCTGTGGGCCTTCGTCTTGGGTCTCTCCAGGGTCATGCTTGGGCGGCACAACGTCAC
TGATGTGGCGTTTGGCTTTTTCCTGGGCTACATGCAGTACAGCATTGTGGATTATTGCTGGCTGTCACCC
CTCAATGTTCCAGTTCTTTTTGTACTGTGGAATCAACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR214851 representing NM_001034854
Red=Cloning site Green=Tags(s)

MPSPRRTIEGRPLGSSGGSSVPGSPAHGGGGGGSGRFEFQSLLSCRSGADPACARLRASDSPVHRRGSFP
LAAACPAQVAPAPPPEDAGMNLNPSFLGIALRSLLAIDLWLSKKLGVCAGESSAWGSVRPLMKLLEISGH
GIPWLLGTLYCLLRSDSWAGREVLMNLLFALLLDLLLVAVIKGLVRRRRPAHNQMDMFFTLSVDKYSFPS
GHATRAALVSRFILNHLVLAIPLRVLVVLWAFVLGLSRVMLGRHNVTDVAFGFFLGYMQYSIVDYCWLSP
LNVPVLFVLWNQQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001034854
ORF Size 879 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001034854.1, NP_001030026.1
RefSeq Size 2874 bp
RefSeq ORF 882 bp
Locus ID 619549
UniProt ID Q66H88
MW 31.7 kDa
Gene Summary Phosphatase that dephosphorylates presqualene diphosphate (PSDP) into presqualene monophosphate (PSMP), suggesting that it may be indirectly involved in innate immunity. PSDP is a bioactive lipid that rapidly remodels to presqualene monophosphate PSMP upon cell activation. Displays diphosphate phosphatase activity with a substrate preference for PSDP > FDP > phosphatidic acid (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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