Elk1 (NM_001108059) Rat Tagged ORF Clone

CAT#: RR212867

  • TrueORF®

Elk1 (Myc-DDK-tagged ORF) - Rat ELK1, member of ETS oncogene family (Elk1), (10 ug)



  "NM_001108059" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Anti-Elk-1 (Ser383) Antibody (Phospho-Specific)
    • 100 ul

CNY 5,643.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR212867 representing NM_001108059
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCATCTGTGACACTGTGGCAGTTTCTGCTGCAGCTTCTAAGAGAACAAGGTAATGGCCACATCA
TCTCCTGGACCTCACGGGATGGTGGTGAGTTCAAGTTGGTGGATGCAGAAGAGGTGGCCCGGCTATGGGG
ACTGCGCAAGAACAAGACCAACATGAATTATGACAAGCTTAGCCGGGCCTTGCGCTACTACTATGATAAG
AATATCATCCGCAAGGTGAGCGGCCAGAAGTTTGTCTACAAGTTTGTGTCCTACCCAGAGGTTGCAGGGT
GCTCCACTGAAGACTGCCCACCCCAGCCTGAGGTGTCTGTAACCTCGGCCGTAGCAATGGCCCCTGCTAC
TGTCCATTCAGGCCCAGGGGACAATGCCACTGGAAAGCCAGGAACACCAAAGGGTGCAGGAATGACAGGC
CAAGGTGGCTTAGCACGAAGCAGCCGGAATGAATACATGCGCTCGGGCCTCTATTCTACCTTCACAATAC
AGTCCCTGCAGCCACAGCCACCCCTTCATCCTCGGCCTGCCTCAGTGCTTCCCAACACTACCCCTGCAGG
AGTACCAGCACCCCCCTCAGGGAGCAGGAGCACCAGTCCAAACCCCTTAGAAGCCTGCTTGGAGGCAGAA
GAGGCTGGTCTGCCCCTGCAGGTTATCTTAACCCCACCAGAGGCCCCAAACCAGAAATCTGAAGAGTTGA
GTCTGAACCCAGGTTTTGGCCGTCCACAACCCCCAGAAGTCAAAGTGGAGGGGCCTAAGGAAGAATTGGA
AGTTACAGAGGTTGGAGGCTTCAGTCCAGAAGCTGTCAAAGCTGAACAAGAAGTCTCACCCTCAGAAGGC
CTGCTGGCTCGGCTCCCAGCCATCCTAACAGAGAATACAGCACAGGTGTGTGGCCTCTCCACCTCCACCA
CTGAGATCACCCAACCCCAGAAGGGCCGGAAGCCCCGAGACCTGGAACTTCCACTTAGCCCAAGCCTGCT
AGGTGGCCAAGGACCCGAACGGACTCCAGGATCAGGAACAAGCTCTGGTCTTCAGGCACAGGGGCCAGCA
CTAACACCATCCTTGCTCCCCACACATACCTTGACCCCGGTGCTGCTGACACCCAGCTCGCTGCCCCCCA
GCATCCATTTCTGGAGCACTCTGAGTCCAATTGCACCGCGTAGTCCAGCCAAGCTCTCCTTCCAGTTTCC
GTCCAGTGGCAGCGCACAGGTGCACATCCCTTCCATCAGCGTGGATGGCCTCTCGACTCCCGTGGTGCTC
TCCCCAGGGCCCCAAAAGCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR212867 representing NM_001108059
Red=Cloning site Green=Tags(s)

MDPSVTLWQFLLQLLREQGNGHIISWTSRDGGEFKLVDAEEVARLWGLRKNKTNMNYDKLSRALRYYYDK
NIIRKVSGQKFVYKFVSYPEVAGCSTEDCPPQPEVSVTSAVAMAPATVHSGPGDNATGKPGTPKGAGMTG
QGGLARSSRNEYMRSGLYSTFTIQSLQPQPPLHPRPASVLPNTTPAGVPAPPSGSRSTSPNPLEACLEAE
EAGLPLQVILTPPEAPNQKSEELSLNPGFGRPQPPEVKVEGPKEELEVTEVGGFSPEAVKAEQEVSPSEG
LLARLPAILTENTAQVCGLSTSTTEITQPQKGRKPRDLELPLSPSLLGGQGPERTPGSGTSSGLQAQGPA
LTPSLLPTHTLTPVLLTPSSLPPSIHFWSTLSPIAPRSPAKLSFQFPSSGSAQVHIPSISVDGLSTPVVL
SPGPQKP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001108059
ORF Size 1281 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001108059.3, NP_001101529.1
RefSeq Size 2779 bp
RefSeq ORF 1284 bp
Locus ID 314436
UniProt ID A4GTP4
MW 45.3 kDa
Gene Summary This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Multiple isoforms are produced from this gene by the use of alternative translational start codons. [provided by RefSeq, Mar 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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