Zfp423 (NM_053583) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Roaz; Znf423
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211954 representing NM_053583 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGGCGGAAGCAGGCGAAGCCGCGATCGGTGAAAGTTGAAGAGGGGGAGGCCTCGGACTTCTCGC TGGCCTGGGATTCCTCTGTGGCAGCAGCGGGAGGCCTAGAAGGAGAGTCAGAGTGTGATCGGAAATCCAG CCGTGCGCTGGAAGACAGGAACAGCGTGACAAGTCAAGAGGAGAGAAACGAGGACGATGAAGATGTGGAA GATGAGTCAATTTACACCTGCGATCACTGTCAGCAGGACTTCGAGTCTCTGGCAGACCTGACGGACCACC GGGCCCACCGCTGTCCTGGAGATGGTGATGACGACCCACAGCTCTCCTGGGTGGCTTCATCTCCCTCCAG CAAGGATGTTGCGTCACCCACGCAGATGATCGGCGATGGTTGTGACCTTGGCCTCGGCGAGGAGGAAGGC GGCACCGGCCTGCCGTACCCTTGCCAGTTCTGCGACAAGTCCTTCATCCGCCTGAGCTACTTGAAGAGGC ATGAACAGATCCACAGCGACAAGCTGCCGTTCAAGTGCACCTTCTGCAGCCGCCTCTTCAAACACAAGAG GAGCCGGGACCGGCACATCAAGCTGCACACAGGCGACAAGAAGTACCACTGCCACGAGTGCGAGGCGGCT TTCTCCCGCAGGGACCACCTCAAGATCCACCTGAAGACCCACAGCTCCAGCAAGCCGTTCAAGTGCAGCG TGTGCAAACGCGGGTTCTCCTCCACCAGCTCCCTGCAGAGCCACATGCAGGCCCACAAGAAGAATAAGGA ACACCTGGCTAAGTCAGAGAAGGAAGCCAAGAAGGACGACTTCATGTGTGACTACTGCGAGGACACCTTT AGCCAGACAGAAGAGCTGGAGAAGCATGTGCTTACCCTCCACCCGCAGCTCTCAGAGAAGGCGGACCTCC AGTGTATCCACTGCCCCGAGGTCTTTGTCGACGAGAGCACGCTGCTGGCCCACATCCACCAAGCTCACGC CAACCAGAAACACAAGTGCCCCATGTGCCCTGAGCAGTTCTCCTCCGTGGAGGGTGTGTACTGCCACCTG GACAGCCACCGGCAGCCTGATTCCAGCAATCACAGTGTCAGCCCCGACCCCGTGCTGGGCAGTGTGGCTT CCATGAGCAGTGCTACACCTGACTCGACGCCCGACCCCGTGCTGGGCAGTGTGGCTTCCATGAGCAGTGC TACACCTGACTCGAGCGCCTCTGTGGAGCGCGGGTCCACGCCAGACTCCACCTTGAAGCCGCTGAGGGGG CAGAAGAAGATGCGGGATGACGGGCAGAGCTGGTCCAAGGTTGTCTACAGCTGCCCCTACTGTTCTAAGC GGGACTTTACCAGCCTGGCTGTGCTAGAGATTCATCTGAAGACCATTCACGCGGACAAACCTCAGCAGAG TCACACGTGTCAGATTTGCCTGGACTCCATGCCCACGCTCTACAACCTCAACGAGCATGTGCGCAAGCTG CACAAGAGCCACGCTTACCCCGTCATGCAATTCGGCAACATCTCCGCCTTCCACTGCAACTACTGCCCCG AGATGTTCGCGGACATCAACAGCCTGCAGGAGCACATCCGAGTCTCGCACTGTGGCCCCAATGCCAACCC CCCCGACGGGAACAATGCTTTCTTCTGTAACCAGTGTTCTATGGGCTTTCTCACTGAATCCTCCCTCACA GAGCACATCCAACAGGCACACTGCAGTGTGGGGAGCACCAAGCTGGAGTCTCCCGTTATCCAGCCCACAC AGTCCTTCATGGAGGTCTACTCCTGCCCTTACTGTACCAACTCCCCTATCTTTGGCTCCATCCTGAAGCT CACTAAGCACATCAAAGAGAACCACAAGAACATCCCGTTGGCACACAGCAAGAAGTCCAAGGCGGAGCAG AGTCCGGTCTCCTCTGACGTCGAGGTGTCTTCCCCGAAACGACAGCGCCTCTCGGGGAGTGCCAACTCCA TCTCTAACGGCGAGTACCCCTGCAATCAGTGCGACCTCAAGTTCTCCAACTTCGAGAGCTTCCAGACCCA CTTGAAGCTGCACCTGGAGCTGCTGCTCCGGAAGCAGGCCTGCCCCCAGTGCAAAGAGGACTTCGACTCT CAGGAGTCCCTCCTGCAGCATCTGACCGTGCACTACATGACCACGTCCACCCACTACGTCTGCGAGAGCT GTGACAAGCAGTTCTCCTCAGTGGACGACCTGCAGAAGCACCTGCTGGACATGCACACCTTTGTGCTATA CCACTGCACCCTGTGTCAGGAGGTCTTCGACTCTAAGGTGTCCATCCAGGTGCACCTGGCCGTGAAGCAC AGCAACGAGAAGAAGATGTACCGTTGCACCGCCTGCAACTGGGACTTCCGCAAGGAGGCTGACCTGCAGG TGCACGTCAAGCACAGTCACCTCGGCAACCCGGCCAAGGCCCACAAGTGCATCTTCTGTGGTGAGACCTT CAGCACCGAGGTGGAGCTCCAGTGCCACATCACCACGCACAGCAAGAAGTACAATTGCAGGTTCTGCAGC AAAGCCTTCCACGCCGTCCTCCTGCTGGAGAAGCACCTTCGGGAGAAGCATTGTGTGTTTGACCCAGCTG CAGAGAATGGCACGGCCAACGGGGTGCCCCCCACCTCCACCAAGAAGGCAGAGCCCGCCGACCTGCAGGG CATGCTGCTCAAGAACCCTGAGGCACCGAACAGCCACGAGGCCAGTGAGGACGATGTGGATGCATCAGAG CCCATGTACGGCTGTGACATCTGTGGTGCAGCCTACACCATGGAGGTGCTGCTGCAGAACCACCGACTCC GGGATCATAACATCCGGCCCGGAGAGGACGATGGCTCACGCAAGAAGGCAGAGTTCATAAAGGGCAGCCA CAAGTGTAACGTGTGCTCTCGGACTTTCTTCTCGGAGAACGGGCTCCGGGAACACCTGCAGACGCACCGG GGCCCTGCCAAGCACTACATGTGTCCCATCTGTGGCGAGCGCTTCCCCTCGCTGCTGACGCTCACTGAGC ACAAGGTGACCCACAGCAAGAGTCTGGACACAGGCACCTGTCGCATCTGCAAAATGCCCCTGCAGAGTGA GGAGGAGTTTATCGAGCACTGCCAGATGCACCCCGACTTGCGGAACTCCCTCACTGGTTTCCGCTGTGTG GTCTGTATGCAGACTGTCACCTCAACCCTGGAGCTCAAGATCCATGGCACCTTTCACATGCAGAAGCTGG CTGGCAGCTCAGCTGCTTCCTCCCCCAATGGCCAGGGGCTGCAGAAGCTCTACAAGTGCGCCCTGTGCCT CAAAGAGTTCCGTAGCAAGCAGGACCTGGTCAGGCTTGACGTCAATGGGCTGCCCTATGGCCTATGTGCC GGCTGCATGGCCCGTAGTGCCAATGGACAGGTGGGTGGCCTGGCCCCACCCGAACCTGCCGACCGGCCCT GCGCTGGCCTCCGCTGCCCTGAATGTAACGTGAAGTTTGAGAGTGCTGAGGACCTGGAGAGCCACATGCA GGTGGACCACCGTGATCTTACCCCAGAGACCAGTGGGCCCCGGAAAGGTGCCCAGACGTCACCAGTGCCC CGGAAGAAGACGTACCAGTGCATCAAGTGCCAGATGACCTTCGAGAACGAGAGAGAGATCCAGATCCACG TCGCCAACCACATGATCGAGGAAGGCATCAACCATGAGTGTAAGCTGTGCAACCAGATGTTCGACTCCCC AGCCAAGCTCCTTTGTCACCTCATCGAACACAGCTTCGAGGGCATGGGTGGTACTTTCAAGTGCCCCGTG TGCTTCACAGTCTTCGTCCAGGCCAACAAGCTGCAACAGCACATCTTCGCCGTGCACGGGCAGGAGGACA AAATCTACGACTGCTCGCAGTGCCCACAGAAGTTTTTCTTCCAGACAGAGTTGCAGAACCACACGATGAG CCAGCACGCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211954 representing NM_053583 Red=Cloning site Green=Tags(s) MSRRKQAKPRSVKVEEGEASDFSLAWDSSVAAAGGLEGESECDRKSSRALEDRNSVTSQEERNEDDEDVE DESIYTCDHCQQDFESLADLTDHRAHRCPGDGDDDPQLSWVASSPSSKDVASPTQMIGDGCDLGLGEEEG GTGLPYPCQFCDKSFIRLSYLKRHEQIHSDKLPFKCTFCSRLFKHKRSRDRHIKLHTGDKKYHCHECEAA FSRRDHLKIHLKTHSSSKPFKCSVCKRGFSSTSSLQSHMQAHKKNKEHLAKSEKEAKKDDFMCDYCEDTF SQTEELEKHVLTLHPQLSEKADLQCIHCPEVFVDESTLLAHIHQAHANQKHKCPMCPEQFSSVEGVYCHL DSHRQPDSSNHSVSPDPVLGSVASMSSATPDSTPDPVLGSVASMSSATPDSSASVERGSTPDSTLKPLRG QKKMRDDGQSWSKVVYSCPYCSKRDFTSLAVLEIHLKTIHADKPQQSHTCQICLDSMPTLYNLNEHVRKL HKSHAYPVMQFGNISAFHCNYCPEMFADINSLQEHIRVSHCGPNANPPDGNNAFFCNQCSMGFLTESSLT EHIQQAHCSVGSTKLESPVIQPTQSFMEVYSCPYCTNSPIFGSILKLTKHIKENHKNIPLAHSKKSKAEQ SPVSSDVEVSSPKRQRLSGSANSISNGEYPCNQCDLKFSNFESFQTHLKLHLELLLRKQACPQCKEDFDS QESLLQHLTVHYMTTSTHYVCESCDKQFSSVDDLQKHLLDMHTFVLYHCTLCQEVFDSKVSIQVHLAVKH SNEKKMYRCTACNWDFRKEADLQVHVKHSHLGNPAKAHKCIFCGETFSTEVELQCHITTHSKKYNCRFCS KAFHAVLLLEKHLREKHCVFDPAAENGTANGVPPTSTKKAEPADLQGMLLKNPEAPNSHEASEDDVDASE PMYGCDICGAAYTMEVLLQNHRLRDHNIRPGEDDGSRKKAEFIKGSHKCNVCSRTFFSENGLREHLQTHR GPAKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGTCRICKMPLQSEEEFIEHCQMHPDLRNSLTGFRCV VCMQTVTSTLELKIHGTFHMQKLAGSSAASSPNGQGLQKLYKCALCLKEFRSKQDLVRLDVNGLPYGLCA GCMARSANGQVGGLAPPEPADRPCAGLRCPECNVKFESAEDLESHMQVDHRDLTPETSGPRKGAQTSPVP RKKTYQCIKCQMTFENEREIQIHVANHMIEEGINHECKLCNQMFDSPAKLLCHLIEHSFEGMGGTFKCPV CFTVFVQANKLQQHIFAVHGQEDKIYDCSQCPQKFFFQTELQNHTMSQHAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053583
ORF Size	3933 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053583.2, NP_446035.2
RefSeq Size	4667 bp
RefSeq ORF	3936 bp
Locus ID	94188
UniProt ID	O08961
MW	147.2 kDa
Gene Summary	zinc finger protein that interacts with the Olf-1/EBF transcription factor; plays a role in regulation of differentiation of olfactory neurons [RGD, Feb 2006]

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