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Mus81 (NM_001025645) Rat Tagged ORF Clone
CAT＃: RR210437
- TrueORF®
Mus81 (Myc-DDK-tagged ORF) - Rat MUS81 endonuclease homolog (S. cerevisiae) (Mus81), (10 ug)
"NM_001025645" in other vectors (3)
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CNY 7,030.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC112911 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210437 representing NM_001025645
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCGGTCCGCTTGGGCCGGAAGCGTCCGCTGCCTGTTTGCCCCAACCCGCTCTTCGTTCGTT GGCTAACCGAGTGGCGGGACGAGGCAGCCAGCAGAGGGCGCCACACGCGCTTTGTGTTTCAAAAGGCACT GCGCTCCCTCCAGCGGTACCCGCTACCATTGCGCAACGGGAAAGAAGCCAAGATACTCCAGCACTTCGGA GACAGGCTCTGTCGCATGCTGGACGAAAGGCTGAAGGAGCACCTAGCATCAGGCGGTGACCATGCCCCCA GCTCACCATCTGGAAAGAAGAGAGCCAGCAAAGGGCCACCTGCCCAGGTCCAGGGCTCTTCCATGCCAGT TCCCACCCAGCCTCAAGCAGGAAGCACCAATGCTGGCTATTGGCCAGCTCAGAACTCAGGTGCTCGAGAG ATCCTGCTGCAGCTCTACAGGGAGCACCTGAATTCTGATGGCCACAGCTTTCTAACCAAAGAGGAGCTGC TGCAGAAATGTGCCCAGAAGACCCCCAGGGTAGTGCCTGAAAGTTCAAGACCCTGGCCTGCCCTCCGAGG GCTCCTCCACAGGAACCTAGTCCTCAGAACTCATCGGCCAGCCAGGTATGCACTGACACCAGAGGGCCTG GAGCTGGCCCAGAAGCTGGCTGAGGCAGAAGGCCTGAGCACTCTGAACACTGCCTTTCAGCCAGAGGAGC ATCATGAAGAGTCACCAGTTCCAGAAGCCATCTTGTCAGAACCTGGCACTACTGAGGTGGGCGTCCAACA GAGACCACTGGAGCTAAGGCCTAGCGAGTACAGAGTGCTGTTGTGTGTGGACATTGGCGAAACCAGAGGG GCAGGACACAGGCCAGAAATGCTCCGAGAGTTACAAAGACTGCGTGTGCCCCATACAGTTCGCAAACTGC ATGTTGGGGACTTTGTGTGGGTGGCACAGGAGACCAGGCCCAGAGACCCAGAAAGACCTGGGGAGTTAGT CCTGGACCACATTGTGGAACGCAAGCGGCTAGATGACCTGTGCAGCAGCATCATTGACGGCCGCTTTCGA GAGCAGAAGTTCCGCCTGAAGCGCTGTGGCCTGGGGCACCGGATATACTTGGTAGAGGAACATGGGTCTG TCCAAAACCTTAGCCTTCCTGAGAGTACATTGCTGCAGGCTGTCACAAACACCCAGGTCATTGATGGTTT TTTTGTGAAGCGTACCATGGATATTAAGGAGTCAGCTGGCTACCTGGCGCTTTTGACAAAGGGCCTGGAA AGACTGTACCAGGGCCACACCCTACACAGCCGCCCTTGGGGAACCCCAGGGGATGCTGAATCAGAAGCAA AGCCTTCCACAAACCCTCTCTGCTCACTCCTCACCTTCAGTGACTTCAATGCAGAAGCTGTCAAGAACAA GGCCCAGTCTGTGCGAGAGGTATTTGCCCGGCAGCTGATGCAGGTGCGTGGACTGAGTGGGGAGAAGGCA GCAGCCCTGGTGGATCGATACAGCACCCCTGCCAGTCTCCTGGCTGCCTATGATGCCTGTGCCACCACCA AGGAGCAGGAGATGCTGCTGAGCACCGTCAAGTGTGGGCGTCTGCAGAGAAATCTGGGACCTGCTCTGAG CAGGACCCTGTACCAGCTGTATTGCAGCCACAGCCCTTTGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210437 representing NM_001025645
Red=Cloning site Green=Tags(s) MAEPVRLGRKRPLPVCPNPLFVRWLTEWRDEAASRGRHTRFVFQKALRSLQRYPLPLRNGKEAKILQHFG DRLCRMLDERLKEHLASGGDHAPSSPSGKKRASKGPPAQVQGSSMPVPTQPQAGSTNAGYWPAQNSGARE ILLQLYREHLNSDGHSFLTKEELLQKCAQKTPRVVPESSRPWPALRGLLHRNLVLRTHRPARYALTPEGL ELAQKLAEAEGLSTLNTAFQPEEHHEESPVPEAILSEPGTTEVGVQQRPLELRPSEYRVLLCVDIGETRG AGHRPEMLRELQRLRVPHTVRKLHVGDFVWVAQETRPRDPERPGELVLDHIVERKRLDDLCSSIIDGRFR EQKFRLKRCGLGHRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGLE RLYQGHTLHSRPWGTPGDAESEAKPSTNPLCSLLTFSDFNAEAVKNKAQSVREVFARQLMQVRGLSGEKA AALVDRYSTPASLLAAYDACATTKEQEMLLSTVKCGRLQRNLGPALSRTLYQLYCSHSPLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025645 |
| ORF Size | 1653 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025645.1, NP_001020816.1 |
| RefSeq Size | 1924 bp |
| RefSeq ORF | 1656 bp |
| Locus ID | 293678 |
| UniProt ID | Q4KM32 |
| MW | 61.9 kDa |
| Gene Summary | Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210437 | Mus81 (untagged ORF) - Rat MUS81 endonuclease homolog (S. cerevisiae) (Mus81), (10 ug) |
CNY 7,030.00 |
|
| RR210437L3 | Lenti ORF clone of Mus81 (Myc-DDK-tagged ORF) - Rat MUS81 endonuclease homolog (S. cerevisiae) (Mus81), (10 ug) |
CNY 8,930.00 |
|
| RR210437L4 | Lenti ORF clone of Mus81 (mGFP-tagged ORF) - Rat MUS81 endonuclease homolog (S. cerevisiae) (Mus81), (10 ug) |
CNY 9,880.00 |
