Arid1a (NM_001106635) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210396 representing NM_001106635 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCAGATGGGAAAGATGAGACCTCAGCCGTATGGTGGGACTAACCCATACTCACAACAACAGGGAC CTCCTTCAGGACCGCAACAAGGACATGGGTACCCAGGGCAGCCATATGGGTCCCAGACTCCACAGCGGTA CCCGATGACCATGCAGGGCCGGGCTCAGAGTGCCATGGGTAGCCTCTCTTATGCACAGCAGATTCCACCT TACGGACAGCAAGGCCCCAGTGCCTATGGTCAGCAGGGCCAGACTCCGTACTATAACCAGCAAAGTCCTC ATCCCCAGCAGCAGCAGCAGCCACCTTACTCCCAGCAGCCACCGTCTCAGACCCCTCATGCCCAGCCTTC ATATCAGCAGCAGCCGCAGACTCAGCAACCACAACTTCAGTCCTCTCAGCCTCCATATTCCCAGCAGCCT TCCCAGCCTCCACATCAGCAGTCCCAAACTCCATATCCCTCCCAGCAGTCCACCACACAACAGCACCCCC AGAGCCAGCCCCCCTACTCACAGCCACAGGCACAGGCTCCCTACCAGCAGCAGCAACCTCAGCAGCCCGC ATCCTCGACGCTCTCCCAGCAGGCCGCGTACCCTCAGCCCCAGCCTCAGCAGTCCCAGCAAACTGCCTAT TCCCAGCAGCGCTTCCCTCCACCACAGGAGCTTTCTCAAGATTCATTTGGGTCTCAGGCATCCTCAGCCC CCTCGATGACCTCCAGCAAGGGAGGGCAAGAAGATATGAACCTGAGTCTTCAGTCAAGACCCTCCAGCTT GCCTGATCTGTCTGGTTCAATCGATGATCTCCCCATGGGGACAGAAGGAGCTCTGAGTCCTGGCGTGAGC ACATCAGGGATTTCCAGCAGCCAAGGAGAACAGAGCAATCCAGCTCAGTCTCCTTTTTCTCCTCACACCT CCCCTCACCTGCCTGGCATCCGAGGCCCGTCCCCGTCCCCTGTTGGCTCTCCTGCCAGTGTCGCTCAGTC TCGCTCAGGACCACTCTCGCCTGCTGCAGTGCCAGGCAACCAGATGCCACCCCGGCCACCCAGTGGTCAG TCGGACAGCATCATGCACCCTTCCATGAACCAATCAAGCATTGCCCAAGATCGAGGTTATATGCAGAGGA ACCCCCAAATGCCCCAGTACACTTCCCCTCAGCCCGGCTCGGCCTTATCTCCACGTCAGCCTTCTGGAGG ACAGATGCACTCGGGCATGGGCTCCTACCAGCAGAACTCCATGGGGAGCTATGGACCCCAGGGGAGTCAG TATGGCCCACAAGGAGGCTATCCTAGGCAGCCTAACTATAACGCCTTGCCCAACGCCAACTACCCCAGTG CAGGCATGGCTGGAAGTATGAACCCGCTGGGTGCTGGAGGTCAAATGCACGGGCAGCCTGGAATCCCACC CTACGGCACACTCCCTCCAGGAAGAATGACTCATGCGTCTATGGGCAACAGGCCTTATGGCCCTAATATG GCCAACATGCCACCTCAGGTTGGGTCAGGGATGTGTCCTCCACCAGGGGGGATGAACCGGAAAACTCAAG AATCTGCTGTTGCCATGCATGTTGCTGCCAACTCTATCCAAAACAGGCCACCAGGCTACCCAAATATGAA TCAAGGAGGCATCATGGGAACTGGACCTCCCTATGGGCAGGGGATTAATAGTATGGCTGGCATGATCAAC CCTCAGGGACCCCCATATCCTATGGGTGGAACCATGGCCAACAATTCAGCAGGGATGGCAGCCAGCCCAG AGATGATGGGCCTTGGGGATGTTAAGTTAACTCCCGCCACAAAAATGAACAACAAGGCAGATGGGACACC CAAGACAGAATCCAAATCTAAGAAGTCCAGTTCTTCTACCACCACCAATGAGAAGATCACCAAATTGTAT GAGTTGGGTGGTGAGCCCGAGAGGAAGATGTGGGTGGACCGGTACCTGGCCTTCACAGAGGAAAAGGCCA TGGGCATGACAAATCTGCCTGCTGTTGGGAGGAAGCCTCTGGACCTCTATCGCCTCTATGTGTCTGTGAA GGAGATTGGTGGGCTGACTCAGGTCAACAAAAACAAAAAATGGCGGGAACTTGCAACCAACCTCAATGTG GGCACATCGAGCAGTGCCGCCAGCTCCTTGAAAAAGCAGTATATCCAGTGTCTCTATGCCTTTGAGTGCA AGATTGAGCGGGGAGAAGACCCTCCCCCTGATATCTTTGCAGCTGCTGATTCCAAGAAGTCCCAACCCAA GATCCAGCCTCCCTCTCCTGCAGGATCAGGGTCTATGCAGGGGCCACAAACGCCTCAGTCAACCAGCAGT TCTATGGCAGAAGGAGGAGACCTGAAGCCACCAACTCCAGCATCCACACCACATAGTCAAATTCCCCCCT TACCAGGCATGAGGAGCAACTCAGTTGGAATCCAGGATGCCTTTCCTGATGGAAGTGAGCCCACATTCCA GAAGCGGAATTCCATGACTCCAAACCCTGGGTACCAGCCCAGTATGAATACCTCTGACATGATGGGGCGC ATGTCCTATGAGCCAAATAAGGATCCTTATGGCGGCATGAGGAAAGCTCCAGGAAGTGATCCCTTCATGT CCTCAGGGCAGGGTCCCAACGGCGGGATGGGTGACCCCTACAGCCGTGCTGCTGGCCCTGGGCTGGGTAA TGTGGCTATGGGACCACGACAGCACTATCCCTATGGAGGTCCTTACGACAGAGTGAGGACGGAGCCTGGA ATTGGCCCTGAAGGAAATATGGGCACTGGAGCCCCTCAGCCAAATCTCATGCCTTCCACCCCAGACTCGG GGATGTATTCTCCTAGCCGCTACCCCCCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA GCAGCAGCAGCAGCGACACGATTCCTATGGCAATCAGTTCTCCACCCAAGGCACCCCTTCCAGCAGTCCC TTCCCCAGCCAGCAGACCACAATGTATCAGCAGCAGCAGCAGCAGAACTATAAGAGGCCAATGGATGGCA CGTATGGCCCTCCTGCCAAGCGGCATGAAGGGGAGCTGTACAGTGTGCCGTACAGCACTGGGCAGGGGCA GCCTCAACAGCAGCAGCTGCCTGCAGCTCAGTCCCAGTCTGCCAGCCAGCCACAAGCTGCCCAGCCTTCC CCTCAGCAGGATGTCTACAACCAGTACAGCAATGCCTACCCTGCTACTGCCACCGCTGCTACTGAGCGCC GACCAGCAGGCGGCCCCCAGAACCAATTTCCATTCCAGTTTGGCCGAGACCGAGTCTCTGCACCTCCTGG TTCCAATGCCCAGCAGAACATGCCACCACAGATGATGGGCGGCCCCATACAGGCATCCGCTGAGGTTGCT CAGCAAGGCACCATGTGGCAGGGGCGAAATGACATGACCTACAATTATGCCAACAGGCAGAACACAGGCT CTGCCCCCCAGGGCCCTGCTTATCATGGTGTGAACCGAACAGATGAGATGCTCCACACAGACCAGAGGGC CAACCATGAAGGCCCGTGGCCTTCCCATGGCACGCGCCAGCCTCCCTATGGTCCTTCAGCCCCTGTTCCC CCCATGACAAGACCCCCTCCATCTAACTACCAGCCCCCACCAAGCATGCAGAATCACATTCCTCAGGTAT CCAGTCCCGCTCCCCTGCCCCGGCCCATGGAGAACCGTACTTCTCCTAGCAAGTCTCCATTCCTGCACTC TGGGATGAAAATGCAAAAGGCAGGTCCACCAGTGCCTGCTTCGCACATAGCGCCTACCCCTGTGCAGCCG CCTATGATCCGGCGGGATATCACCTTTCCCCCTGGCTCTGTGGAGGCCACACAGCCTGTGCTGAAGCAGA GAAGGCGGCTCACGATGAAAGACATTGGAACCCCGGAGGCATGGCGGGTAATGATGTCCCTCAAGTCCGG GCTCCTGGCAGAGAGCACGTGGGCGTTAGACACCATTAACATCCTACTGTATGATGACAACAGCATCATG ACCTTCAACCTCAGCCAGCTCCCAGGGTTGCTAGAGCTCCTTGTTGAATATTTCCGTCGATGCCTGATTG AAATCTTTGGCATTTTAAAGGAGTATGAGGTAGGGGACCCAGGACAAAGAACACTGCTAGACCCTGGGAG ATTGACCAAGGTGTCTAGTCCGGCCCCCACAGAGGAAGAAGAGGAAGAGGAACACGTAGATCCCAAGCTG GAGGAGGAAGAGGAGGAAGGGGTTGAAAATGATGAGGAAATGGCCTTTTTGGGCAAGGACAAGCCACCTT CAGAGAAGAGTGAGGAGAAGCTAGTCAGTAAGTTTGACAAGCTCCCGGTAAAGATCGTGCAGAAGAATGA CCCGTTTGTGGTGGACTGCTCAGATAAGCTTGGGCGCGTGCAGGAGTTTGACAGTGGCCTGCTGCACTGG CGGATTGGTGGTGGGGATACCACTGAGCACATCCAGACCCACTTTGAGAGCAAGATAGAGCTGCTGCCTT CCCGGCCTTGTGTGCCCTCCCCAGTGCCCCCTCGGAAACACGTCACAACAGTAGAGGGTACACCAGGGAC AACGGAGCAGGAGGGTCCCCCGCCTGATGGCCTTCCAGAAAAAAGGATCACAGCCACTATGGATGACATG CTGTCCACCCGGTCTAGCACATTGACTGACGAGGGAACAAAGAGTACAGAGGCCAACAAGGAAAGCAGCA AGTTTCCATTTGGCATTAGCCCAGCACAGAGCCACCGGAACATCAAGATTTTAGAGGACGAACCCCATAG TAAGGATGAGACCCCACTGTGTACCCTTCTGGACTGGCAGGATTCCCTTGCTAAGCGCTGTGTCTGTGTC TCTAATGCCATCCGGAGCCTGTCATTTGTGCCAGGCAACGACTTTGAGATGTCCAAACACCCAGGGCTGC TGCTTATCCTGGGCAAGCTGATCCTCCTGCACCATAAGCACCCAGAACGGAAGCAGGCGCCACTGACTTA TGAGAAGGAGGAGGAACAGGACCAAGGGGTGAGCTGTGACAAAGTGGAATGGTGGTGGGACTGCTTGGAG ATGCTCCGGGAAAACACTCTGGTCACCCTTGCCAACATCTCGGGGCAATTGGACCTATCCCCATACCCTG AGAGCATCTGCCTGCCTGTCCTGGATGGACTCCTACACTGGGCAGTTTGCCCTTCAGCTGAAGCCCAGGA CCCCTTCTCAACCCTAGGCCCCAATGCCGTCCTCTCTCCTCAGAGATTGGTCTTGGAAACCCTCAGCAAA CTCAGCATCCAGGACAACAATGTGGACCTGATACTGGCCACGCCCCCTTTTAGCCGCCTGGAGAAGTTGT ATAGTACCATGGTGCGCTTCCTCAGTGACCGAAAGAACCCAGTGTGCCGGGAGATGGCCGTGGTACTGCT GGCAAATCTGGCACAGGGGGACAGCCTGGCAGCCCGGGCCATTGCGGTGCAGAAGGGCAGCATCGGCAAC CTCCTGGGTTTTCTGGAGGACAGCCTTGCTGCCACCCAGTTCCAGCAGAGCCAGGCCAGCCTCCTCCATA TGCAGAGCCCACCCTTTGAACCAACTAGTGTGGACATGATGCGGCGGGCTGCCCGAGCGCTACTCGCCCT GGCCAAGGTGGACGAGAACCACTCAGAGTTCACTCTGTACGAGTCACGGCTGTTGGACATCTCCGTGTCA CCACTGATGAACTCACTGGTTTCACAAGTCATTTGCGATGTACTGTTTTTGATTGGCCAGTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210396 representing NM_001106635 Red=Cloning site Green=Tags(s) MDQMGKMRPQPYGGTNPYSQQQGPPSGPQQGHGYPGQPYGSQTPQRYPMTMQGRAQSAMGSLSYAQQIPP YGQQGPSAYGQQGQTPYYNQQSPHPQQQQQPPYSQQPPSQTPHAQPSYQQQPQTQQPQLQSSQPPYSQQP SQPPHQQSQTPYPSQQSTTQQHPQSQPPYSQPQAQAPYQQQQPQQPASSTLSQQAAYPQPQPQQSQQTAY SQQRFPPPQELSQDSFGSQASSAPSMTSSKGGQEDMNLSLQSRPSSLPDLSGSIDDLPMGTEGALSPGVS TSGISSSQGEQSNPAQSPFSPHTSPHLPGIRGPSPSPVGSPASVAQSRSGPLSPAAVPGNQMPPRPPSGQ SDSIMHPSMNQSSIAQDRGYMQRNPQMPQYTSPQPGSALSPRQPSGGQMHSGMGSYQQNSMGSYGPQGSQ YGPQGGYPRQPNYNALPNANYPSAGMAGSMNPLGAGGQMHGQPGIPPYGTLPPGRMTHASMGNRPYGPNM ANMPPQVGSGMCPPPGGMNRKTQESAVAMHVAANSIQNRPPGYPNMNQGGIMGTGPPYGQGINSMAGMIN PQGPPYPMGGTMANNSAGMAASPEMMGLGDVKLTPATKMNNKADGTPKTESKSKKSSSSTTTNEKITKLY ELGGEPERKMWVDRYLAFTEEKAMGMTNLPAVGRKPLDLYRLYVSVKEIGGLTQVNKNKKWRELATNLNV GTSSSAASSLKKQYIQCLYAFECKIERGEDPPPDIFAAADSKKSQPKIQPPSPAGSGSMQGPQTPQSTSS SMAEGGDLKPPTPASTPHSQIPPLPGMRSNSVGIQDAFPDGSEPTFQKRNSMTPNPGYQPSMNTSDMMGR MSYEPNKDPYGGMRKAPGSDPFMSSGQGPNGGMGDPYSRAAGPGLGNVAMGPRQHYPYGGPYDRVRTEPG IGPEGNMGTGAPQPNLMPSTPDSGMYSPSRYPPQQQQQQQQQQQQQQQQQQRHDSYGNQFSTQGTPSSSP FPSQQTTMYQQQQQQNYKRPMDGTYGPPAKRHEGELYSVPYSTGQGQPQQQQLPAAQSQSASQPQAAQPS PQQDVYNQYSNAYPATATAATERRPAGGPQNQFPFQFGRDRVSAPPGSNAQQNMPPQMMGGPIQASAEVA QQGTMWQGRNDMTYNYANRQNTGSAPQGPAYHGVNRTDEMLHTDQRANHEGPWPSHGTRQPPYGPSAPVP PMTRPPPSNYQPPPSMQNHIPQVSSPAPLPRPMENRTSPSKSPFLHSGMKMQKAGPPVPASHIAPTPVQP PMIRRDITFPPGSVEATQPVLKQRRRLTMKDIGTPEAWRVMMSLKSGLLAESTWALDTINILLYDDNSIM TFNLSQLPGLLELLVEYFRRCLIEIFGILKEYEVGDPGQRTLLDPGRLTKVSSPAPTEEEEEEEHVDPKL EEEEEEGVENDEEMAFLGKDKPPSEKSEEKLVSKFDKLPVKIVQKNDPFVVDCSDKLGRVQEFDSGLLHW RIGGGDTTEHIQTHFESKIELLPSRPCVPSPVPPRKHVTTVEGTPGTTEQEGPPPDGLPEKRITATMDDM LSTRSSTLTDEGTKSTEANKESSKFPFGISPAQSHRNIKILEDEPHSKDETPLCTLLDWQDSLAKRCVCV SNAIRSLSFVPGNDFEMSKHPGLLLILGKLILLHHKHPERKQAPLTYEKEEEQDQGVSCDKVEWWWDCLE MLRENTLVTLANISGQLDLSPYPESICLPVLDGLLHWAVCPSAEAQDPFSTLGPNAVLSPQRLVLETLSK LSIQDNNVDLILATPPFSRLEKLYSTMVRFLSDRKNPVCREMAVVLLANLAQGDSLAARAIAVQKGSIGN LLGFLEDSLAATQFQQSQASLLHMQSPPFEPTSVDMMRRAARALLALAKVDENHSEFTLYESRLLDISVS PLMNSLVSQVICDVLFLIGQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106635
ORF Size	5733 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106635.1, NP_001100105.1
RefSeq Size	7063 bp
RefSeq ORF	5736 bp
Locus ID	297867
MW	207.5 kDa

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