Gprasp1 (NM_134386) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	pips
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207475 representing NM_134386 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGGGGCAGAGGTTGAGCCTGGTGCCCAGGCAAAGGCTGAAAATAAGCCTGGGGATGAGAATGCTA ATGCTGCTGAAGTAGAGCCTGAAGTTCCGTTAGTGGTCAGACCCAAGGTTAGGACACAAATGATGACTGG AGCAAGACCCAAAGTGAAGCCTAAGGGTACCCCTGGAGCAAGGCCTAAAGGTGAAACCAGTTCACCAGGT GGGGCATATGCTAAATGTAAGCCCAGGTCAATCCCTATTTCACGATCTAAGCATGATGCACAAGTCTGGG CCCCGAGTAAGTTTCGTGGTGAGTCCATGTCAAAGATGGGGAAGCAATGTCAGATCAGTGCTGCAGACTC CCCACTGGTCAGTAATGATTCTGGGGCGGTTGCTCAAGCTAAGTGCCTGTCTGTAGATAGAGAACTTGCT AATATGGACACTGAGAGTATTCCCAAAAAGGCCAGTTCTCCAGCAAGATTCCAGCCTTCATTTGGCCCAG AGGAGGGAACCAGTATGGGATCCTGGTACCGTCCCAGGCCTATCCCTAAAGGAGAGGCATATGAGAATTC TGATTTCAAATGGGCAGATAAATCCTCAGGAAGTTCCTCCTTCTGGAATAGAGATGAAACCAGTACAAGA TTTCGTCCTAGGAAAAGCATGAAATCCAATACCAGATTCAGGCACATGGCCAAACAAGAGGCAAATACCA TGTCTAGACACAAGAACAAACAGGAGTTCTATAATATTTCCAGTTCTGATTCTGAAGATGAGTCTGCTAA GACTCCCTGGTTCTGGGCCAAAGACAAGCCCAAAGTCTGGTCTAGGCCCAAAGAAGAGCCCAATACTAGG TCCTGGTTTAGGTCTAAGAAAGAAGTCCGTGTTGAATCCACTTCTGGGTCTGAATGTGAAAACCATACAA AATCTTTGTTTTGGTCTGGAGAAGAGGCTAAATGTAGGTCTAAACCCAGAGCCAGGAAAGGGGTCAATAT GAGGGCCAGGCACCAGGCCAAGCGAGAAGCTTATAGTGATGTCACATCTGGATCTGTAGATAAAAATAAG AAAGATTCCTGGTTCTTGCCTGAAGAGAAAGCTAATGCCTTTTCAAAGTCTAAGACAAAGAAAGAGCCCA GAACCAGAGCAATGCCAAGGGAAGAAGTCAAAACTAAGGCCAGAGCAAGTACCAAACAAGAAGCCAGGCC AGAGGAGGAAGTCCTCGTTGGGGCTTGGGTTCTGGACACCCAGGACAACACTATGGGGGAGAGGATAAGC ATGAAGACCACTTGTGTAGAGGAGGAGCCTATTGTTGGAGATTGGTTCTGGAGTGAAGAAGAAGCCAGTG TGGACTCTGAGACCGGTCTTAAATCCAGACCAAGGGCTAAGGAGGAACAAGTTAGTAGTTTCTGTTTAGG ATCTGGAAAGAAGACCAGTATGGAAAGTGGGCCAAAGGCTACTTCTAAATCTATGCCAGTAGCTAAGGAT GATGAGGTCATTATTGGATCCTGGTTCTGGGCTGATGATGAAGAGATCAGCTTACAGGCTGATGATGAGT CTATTTTTGGATCTTGGTTCTGGGGCACTGGTGAGAAAAGCCTTAGATCTGTTGGAGTCAGTTGTGAGAA GATGCCCAAGTCTGGGGAAAAAGAAGTTACTGATTCCTGGTTCTGGGCTGGAGAAGTCAATACTGAGGCT GAGATGGAGGAACAGGCCAGTTCAGCATCCACAAAAGGAACAATCTTTGTGCCTTGGTTTTGGTCTGAAA AGCAAGCACATATGGATTTAGGAACCGAACCTTGCTCTGACATCATGGCAGGAGCTGAGGAGGAGCCCAT AATTGGGCCCTGGTTCTGGGCTAAAGTAGATAATAGTGTGGAGGCTGAAGTTAATAGTAAGTCTAGCCTT GAAGATGAGGAAGAACCCATTAGATCACCTTGGTTTGGGGCCAGAGAACAACCCAATATGAAGTATGCAG CTGGTGTCGGATACAAGCCTATGGCAGAAGCCGAGGAGGCTAACAAAAAGTCGTGTGTCTGGGCAAAAGA GCCCTGTTTGTATCCTACCAATAGAGAAAGCTTGAAATCTACTCTGGGAGAGAAAGAGGACACTGTTGAT CCATGGCTCTGGTCCAATAATTACCCAAGGACCGAGACCATCACAGGGTCCTGGCTCTGGGCTGCAGAAG AGGGAAATATAGATGATGAGACTGGAGAAGAAATCAAACTACCAACTTTAGAGGACAATGTATTCAATTC TTGGTCCTGGAAAGAAAATGAAGAAACCGTTGTAGAGGCTCCCAACAGAGAAGAGTCCAAGCCAGAAGCG GAAGAGGAAGATATTATTGGCTCTTGGTTTTGGGCTGGGGATGAAGACAGGTTTCAGCCAGCTGCTAAGA TTAAGGAAGAGAACAAGATAGCACCTGAAGATGAAGATACAGTTGGATCCTGGTTCTGGGGCAAGGAAGA GGCCAGTGTAGAGGCAGTGAAAGGGGGTACTTTTGAGTCTGTGTCTGGAATTAAGGAAGAGAAAGCTACT GGGTCGTGGTTCTGGACTGATAAGGCCAAAATAGGGGCTGGGCCACAGACTGTCGAAACTGGGTCAGAAA CTGAAGATGAGGCAATTTTTGAGTCCCTGATCTGGGCCGCAAAAAAGGACAGTATGCAAACAGGAGTAAA CCGTGTGTCCAAGCCAAAGGATGAGGGTGAAGGGATTGAGTCCTGGCTCTGGTCTGGTGACAAGGCCACT ACAGAATCTAAAACTGTGACGGTCAGTGAGTCTAGTCCAGAAAATGGGAAAGAGTCCATTGTTAAGTTTG GGTCAAGAGCAAAGGATGAAGTGATTAACAAGACTGGCAGCGGTGACAATTGTAAATTTAGTACAGAAGC TGAAAGCATAGTGGGACCCTGGTTCTGGGAAGGAGATGAAGCTAGCTTTGAATCAAACCCTGTTCCTGTA TGCAAGGCCGCTTGTGAACCTGAGTCTTCAACTGAACATGAACCTGATCCTTCCCGCAGGCCTCAGAGCT GGGATGAGGTCACTGTTCAGTTTAAGCCTGGTCCATGGGGAAAGGCTGGCTTCCCATCCTTAAACCCCTT CCGATTCCCAAAAGAAGCAGCATCTCTGTTTGCAGAAATGTTTGGGGGAAAGCCTAAGCTTGTGGAAGTT GGTACTGAAAGAGAGCCTGAACCCCAGTTCCCATTTCAGTATGATCCTTCTTACCGGTCAGTCCGGGAAA TTCGGGAGCATCTTAAGGCCAGGGAGAGTGCACAGGCTGAGAACTGGTCTTGCAACTGCATCCAGTGTGA GCTTAGAATTGGTTCTGAGGAGTTTGAGGAACTTCTTTTAATGATGGACAGAAATCGTGATCCATTTATC CATGAGATATCTAAAATTGCAATGGGCATGAGAGGTGCTTCTCAGTTTACCCGAGATTTCATTCGAAACT CGGGTGTTATCTCACTTATTGAAGCCCTGATGAATTATCCTTCCTCCCGAGCGAGGACTGCCTTTTTGGA AAACATGATTCAAATGGCTCCAACTTATCCAGACCTAAACATGATCGAGACATATGTGTGTCAGGTGTGT GAGGATACCTTTGATTATGACTTGGATTCCTCTGACCAGTTGTCTGGACTGACAATGATTACACACCTCA CTACTACTTTTGACTATCACAAAGTGGTTGTCGCTTACTTGGCTGGCTTTTACTACCTACTGAATTCGGG CAATACCACAACAAGGTTTCATGTTCTGAAACTGCTACTAAATTTGTCTGAAAGCCTTGTCATGACAAAA CGCCTACTTATTACTGATTCAGTGTCAGAATTTATGGCCCTTTTTAATAGGGAGGACTCAGATGAAAACA TTCAAATTATTCTTGCAATATTTGAGAATATCAGTAAAAATATTCAAAAAGAGGCACTGTTTGCTGATGA TGAGGAAGAGGAGGAGGAGGAGGAGGCGGTGAATCTTGAACCGCTTATTTCAGCATTCCGTGAGGCTGAA AAATTTGCCAAGGAACTGAAACGTAAAACCGACGATCAGAAATCACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207475 representing NM_134386 Red=Cloning site Green=Tags(s) MTGAEVEPGAQAKAENKPGDENANAAEVEPEVPLVVRPKVRTQMMTGARPKVKPKGTPGARPKGETSSPG GAYAKCKPRSIPISRSKHDAQVWAPSKFRGESMSKMGKQCQISAADSPLVSNDSGAVAQAKCLSVDRELA NMDTESIPKKASSPARFQPSFGPEEGTSMGSWYRPRPIPKGEAYENSDFKWADKSSGSSSFWNRDETSTR FRPRKSMKSNTRFRHMAKQEANTMSRHKNKQEFYNISSSDSEDESAKTPWFWAKDKPKVWSRPKEEPNTR SWFRSKKEVRVESTSGSECENHTKSLFWSGEEAKCRSKPRARKGVNMRARHQAKREAYSDVTSGSVDKNK KDSWFLPEEKANAFSKSKTKKEPRTRAMPREEVKTKARASTKQEARPEEEVLVGAWVLDTQDNTMGERIS MKTTCVEEEPIVGDWFWSEEEASVDSETGLKSRPRAKEEQVSSFCLGSGKKTSMESGPKATSKSMPVAKD DEVIIGSWFWADDEEISLQADDESIFGSWFWGTGEKSLRSVGVSCEKMPKSGEKEVTDSWFWAGEVNTEA EMEEQASSASTKGTIFVPWFWSEKQAHMDLGTEPCSDIMAGAEEEPIIGPWFWAKVDNSVEAEVNSKSSL EDEEEPIRSPWFGAREQPNMKYAAGVGYKPMAEAEEANKKSCVWAKEPCLYPTNRESLKSTLGEKEDTVD PWLWSNNYPRTETITGSWLWAAEEGNIDDETGEEIKLPTLEDNVFNSWSWKENEETVVEAPNREESKPEA EEEDIIGSWFWAGDEDRFQPAAKIKEENKIAPEDEDTVGSWFWGKEEASVEAVKGGTFESVSGIKEEKAT GSWFWTDKAKIGAGPQTVETGSETEDEAIFESLIWAAKKDSMQTGVNRVSKPKDEGEGIESWLWSGDKAT TESKTVTVSESSPENGKESIVKFGSRAKDEVINKTGSGDNCKFSTEAESIVGPWFWEGDEASFESNPVPV CKAACEPESSTEHEPDPSRRPQSWDEVTVQFKPGPWGKAGFPSLNPFRFPKEAASLFAEMFGGKPKLVEV GTEREPEPQFPFQYDPSYRSVREIREHLKARESAQAENWSCNCIQCELRIGSEEFEELLLMMDRNRDPFI HEISKIAMGMRGASQFTRDFIRNSGVISLIEALMNYPSSRARTAFLENMIQMAPTYPDLNMIETYVCQVC EDTFDYDLDSSDQLSGLTMITHLTTTFDYHKVVVAYLAGFYYLLNSGNTTTRFHVLKLLLNLSESLVMTK RLLITDSVSEFMALFNREDSDENIQIILAIFENISKNIQKEALFADDEEEEEEEEAVNLEPLISAFREAE KFAKELKRKTDDQKSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_134386
ORF Size	4038 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_134386.1, NP_599213.1
RefSeq Size	6400 bp
RefSeq ORF	4041 bp
Locus ID	171407
UniProt ID	Q920R4
MW	151.3 kDa
Gene Summary	may mediate feedback loop or output mechanism of circadian rhythm through interacting with Per1 in the SCN [RGD, Feb 2006]

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