Notch2 (NM_024358) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207443 representing NM_024358 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGCTCTGCGTCCCGCCGCGCTGCGGGCGCTGCTGTGGCTCTGGCTGTGCGGCGCGGGCCCCGCGC ACGCTTTGCAGTGTCGAGGTGGTCAAGAGCCCTGTGTAAATGAGGGGACCTGTGTTACCTACCACAACGG CACAGGCTACTGCCGATGTCCAGAGGGCTTCTTGGGAGAATATTGTCAACATCGAGACCCTTGTGAGAAG AACCGCTGTCAGAATGGTGGTACTTGTGTGACGCAGGCCATGTTGGGAAAAGCCACCTGTCGATGTGCTC CAGGGTTCACAGGGGAGGACTGCCAATACTCGACCTCTCACCCCTGTTTTGTTTCCCGCCCCTGTCAGAA TGGAGGTACCTGCCACATGCTCAGCTGGGACACCTATGAGTGCACCTGTCAAGTTGGCTTCACAGGAAAG CAGTGTCAGTGGACAGATGTCTGTCTGTCTCATCCCTGTGAAAATGGAAGCACCTGTAGCTCTGTGGCCA ACCAGTTCTCCTGCAGATGTCCTGCAGGCATCACAGGCCAGAAGTGTGACGCCGACATCAATGAATGTGA CATTCCAGGACGCTGCCAACATGGAGGCACCTGCCTCAACCTTCCTGGGTCCTACCGATGCCAATGCCCT CAGGGCTTCACAGGCCAGCACTGTGACAGCCCGTACGTGCCCTGTGCACCCTCACCCTGCGTCAATGGAG GCACCTGCCGTCAGACTGGAGACTTCACTTCTGAATGCCATTGCCTGCCAGGCTTTGAAGGGAGCAACTG CGAGCGGAATATCGACGACTGCCCTAACCACAAGTGTCAGAATGGAGGGGTGTGTGTGGATGGCGTCAAT ACTTACAACTGCCGCTGCCCCCCTCAGTGGACTGGGCAGTTCTGCACAGAAGACGTGGATGAGTGTCTGC TGCAGCCCAATGCTTGTCAGAATGGAGGCACTTGCACCAACCGCAACGGAGGCTACGGCTGCGTGTGCGT GAACGGCTGGAGTGGGGATGACTGCAGCGAGAACATCGATGACTGTGCCTTCGCTTCCTGCACGCCAGGC TCCACCTGTATTGACCGTGTGGCCTCCTTCTCCTGCCTTTGTCCAGAGGGAAAGGCAGGGCTCCTGTGTC ATCTGGATGATGCCTGTATCAGCAACCCTTGTCACAAGGGGGCGCTGTGTGATACCAACCCCCTGAATGG GCAGTACATTTGCACCTGCCCACAGGGCTACAAGGGCGCTGACTGCACAGAAGACGTGGATGAGTGTGCT ATGGCCAACAGTAACCCTTGTGAGCATGCAGGAAAGTGTGTGAATACAGATGGCGCCTTCCACTGCGAGT GTCTGAAGGGCTACGCAGGGCCTCGCTGTGAGATGGACATCAACGAGTGTCACTCAGACCCCTGTCAGAA CGACGCCACCTGCCTGGATAAGATTGGAGGCTTCACCTGTCTCTGCATGCCGGGTTTCAAAGGTGTGCAT TGTGAACTGGAGGTGAATGAATGCCAGAGCAACCCGTGTGTAAACAATGGGCAGTGTGTGGACAAAGTCA ATCGCTTCCAGTGTCTGTGTCCCCCTGGTTTCACAGGACCAGTGTGCCAGATCGACATTGACGACTGCTC CAGTACTCCCTGCCTGAATGGGGCCAAGTGCATCGATCACCCGAATGGCTATGAATGCCAGTGTGCCACA GGATTCACTGGCACACTGTGTGATGAGAACATCGACAACTGTGACCCGGATCCTTGCCACCATGGCCAGT GCCAGGATGGGATTGACTCCTACACCTGCATCTGCAACCCCGGGTACATGGGAGCCATCTGTAGTGACCA GATTGATGAATGCTACAGCAGCCCCTGCCTGAATGATGGACGCTGCATCGACCTGGTGAACGGCTACCAG TGCAACTGCCAACCGGGTACCTCAGGCCTTAATTGTGAAATTAATTTTGATGACTGTGCCAGCAACCCTT GTCTGCACGGAGCCTGTGTGGACGGCATCAACCGTTACAGTTGTGTGTGCTCTCCGGGATTCACAGGGCA GAGGTGCAACATAGACATTGATGAGTGTGCCTCCAACCCCTGTCGCAAGGGTGCGACGTGCATCAATGAC GTGAATGGTTTCCGGTGTATGTGCCCTGAGGGACCACACCATCCCAGCTGCTACTCACAGGTGAACGAGT GTTTGAGCAGTCCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGCTATAAGTGCCTCTGCGATGC AGGCTGGGTTGGTATCAACTGCGAAGTGGACAAAAATGAGTGTCTTTCTAACCCGTGCCAGAATGGAGGG ACATGTAATAACCTGGTGAATGGCTACAGGTGTACATGCAAGAAGGGGTTCAAAGGCTATAACTGCCAGG TGAACATAGATGAGTGTGCCTCGAACCCGTGTCTGAACCAAGGGACCTGCCTCGATGACGTCAGTGGCTA CACCTGCCACTGCATGCTGCCTTACACAGGCAAGAATTGTCAAACGGTGTTGGCGCCCTGCTCCCCTAAC CCGTGTGAGAACGCTGCAGTTTGTAAAGAGGCACCCAACTTTGAGAGCTTCACCTGCCTGTGTGCCCCTG GCTGGCAAGGTCAGCGCTGTACAGTTGACGTTGATGAGTGTGTCTCCAAGCCGTGTATGAACAATGGCAT CTGCCATAATACTCAGGGCAGCTACATGTGCGAGTGCCCTCCCGGCTTCAGTGGTATGGACTGTGAGGAG GACATCAATGACTGCCTTGCCAACCCCTGCCAGAACGGAGGCTCCTGTGTGGACAAAGTGAACACCTTCT CCTGCCTGTGCCTTCCTGGCTTCGTAGGGGACAAGTGCCAAACAGACATGAATGAATGTCTGAGCGAGCC CTGTAAGAATGGGGGGACCTGCTCTGACTACGTCAACAGCTACACCTGCACGTGCCCTGCGGGCTTCCAT GGAGTCCACTGTGAAAACAACATCGATGAGTGCACTGAGAGCTCCTGTTTCAATGGCGGCACGTGTGTTG ATGGGATCAACTCTTTCTCTTGCTTATGCCCTGTGGGTTTCACTGGTCCCTTCTGCCTCCATGATATCAA TGAGTGCAGCTCTAACCCGTGCCTGAATTCGGGAACGTGTGTTGATGGCCTGGGTACCTACCGATGCACC TGTCCCTTGGGCTACACTGGGAAAAACTGTCAGACCCTGGTGAACCTCTGCAGCCCCTCTCCATGTAAAA ACAAAGGAACTTGTGCTCAGGAAAAGGCAAGGCCACGCTGCCTGTGTCCGCCTGGATGGGATGGCGCATA CTGTGATGTGCTCAATGTGTCCTGTAAGGCGGCAGCCTTGCAGAAAGGAGTACCTGTTGAACACTTGTGC CAGCACTCGGGTATCTGTATCAATGCTGGCAACACGCATCACTGCCAGTGCCCCCTGGGCTACACGGGGA GCTACTGCGAGGAACAGCTTGACGAGTGTGCGTCCAATCCATGCCAGCATGGTGCCACCTGCAGTGACTT CATCGGAGGATACAGATGTGAGTGTGTTCCAGGGTATCAGGGTGTCAACTGTGAGTATGAAGTGGACGAG TGCCAGAACCAGCCCTGTCAGAACGGAGGCACCTGCATCGACCTCGTGAACCATTTCAAGTGCTCGTGCC CACCAGGCACCCGGGGCCTGCTTTGTGAAGAGAACATTGATGACTGTGCTGGGGGCCCCCACTGCCTTAA TGGTGGCCAGTGTGTGGACCGGATTGGAGGCTACAGTTGTCGCTGTTTGCCTGGCTTTGCTGGGGAGCGG TGTGAGGGGGACATCAATGAATGCCTGTCCAATCCTTGCAGCTCAGAGGGCAGCCTGGACTGCATTCAGC TCAAAAATAACTACCAGTGTGTCTGCCGCAGCGCCTTCACAGGCCGACACTGCGAAACCTTCCTAGATGT GTGTCCCCAGAAGCCTTGCCTGAATGGAGGGACTTGTGCTGTGGCTAGCAACGTGCCTGATGGCTTCATT TGTCGTTGTCCCCCAGGGTTCTCCGGGGCAAGATGCCAGAGCAGCTGTGGACAAGTGAAGTGCAGAAGAG GGGAGCAGTGTGTGCACACCGCCTCGGGACCCCACTGCTTCTGCCTGAACCGCAAGGACTGCGAGTCAGG TTGCGCTAGTAACCCCTGCCAGCACGGAGGCACCTGCTACCCTCAGCGCCAGCCTCCTTACTACTCTTGC CGCTGCTCCCCACCGTTCTGGGGCAGCCACTGCGAGAGCTACACAGCCCCCACCAGCACCCCTCCTGCTA CCTGTCTGAGTCAGTACTGTGCCGACAAGGCTCGGGACGGCATCTGTGATGAGGCCTGCAACAGTCATGC CTGCCAGTGGGATGGAGGTGACTGTTCCCTCACTATGGAGGACCCCTGGGCCAACTGCACCTCCTCACTT CGCTGCTGGGAGTACATCAACAACCAGTGTGATGAGCTGTGCAACACTGCAGAGTGCCTGTTTGACAACT TTGAATGCCAGAGGAATAGCAAAACCTGCAAGTATGACAAATACTGTGCAGACCACTTCAAAGACAATCA CTGCGATAAGGGATGCAACAACGAGGAGTGTGGCTGGGACGGGCTGGACTGCGCTGCGGACCAGCCTGAG AACCTGGCGGAGGGCATCCTGGTCATCGTGGTGCTCCTACCCCCTGAGCAGCTGCTTCAGGATTCTCGAA GCTTCTTGAGGGCCCTGGGCACCCTGCTCCACACCAACTTACGCATTAAACAAGACTCTCAGGGCGCCCT CATGGTGTACCCCTACTATGGGGAGAAGTCAGCTGCCATGAAGAAGCAGAAGGTGGCACGCAGGTCTCTT CCTGATGAGCAGGAACAGGAGATAATAGGCTCTAAGGTATTTCTGGAAATCGACAACCGACAGTGTGTTC AAGACTCAGACCAGTGCTTCAAGAACACAGATGCTGCAGCTGCTCTCCTGGCTTCTCATGCCATCCAAGG GACCTTGTCCTACCCTCTGGTGTCTGTTGTCAGTGAATCGGAGGATCCAAGGAACACTCCGCTCCTCTAT CTGCTTGCGGTTGCTGTGGTCATTATCCTGTTCCTCATCCTGCTGGGGGTCATCATGGCAAAGCGGAAGC GCAAGCACGGCTTCCTCTGGCTGCCTGAAGGCTTCACGCTTCGCCGAGACTCCAGCAATCACAAGCGCCG TGAACCTGTGGGGCAGGATGCCGTGGGGCTGAAGAATCTCTCAGTGCAAGTGTCAGAGGCAAACCTGATC GGCTCCACGACAAGCGAGCATTGGGGTGATGATGAAGGACCCCAGCCAAAGAAAGCCAAGGCTGAGGACG ACGAGGCTTTGCTGTCGGAAGACGACCCTGTCGACCGGCGCCCCTGGACACAGCAGCACCTTGAAGCTGC AGACATCCGCCGGACTCCATCCCTGGCGCTCACCCCTCCTCAGGCAGAGCAGGAGGTGGACGTGCTGGAC GTGAATGTCCGAGGCCCAGATGGGTGCACCCCACTGATGCTGGCTTCTCTCCGAGGAGGCAGCTCAGACC TGAGTGATGAAGATGAAGATGCTGAGGACTCTTCTGCCAACATCATCACAGACTTGGTCTACCAAGGTGC CAGCCTCCAGGCACAGACGGACCGCACTGGTGAGATGGCCCTGCACCTTGCAGCCCGCTATTCGAGAGCT GATGCTGCCAAACGCCTCCTGGATGCTGGTGCAGATGCCAACGCCCAGGACAACATGGGCCGCTGTCCTC TTCATGCTGCAGTTGCAGCGGATGCCCAAGGTGTCTTCCAGATTCTGATCCGCAACCGAGTAACGGATCT AGACGCCAGGATGAATGATGGCACTACCCCCCTGATCCTGGCTGCCCGCCTGGCTGTGGAAGGAATGGTG GCAGAGCTGATCAACTGCCAAGCAGATGTGAACGCAGTGGATGACCATGGAAAATCTGCCCTCCACTGGG CAGCCGCCGTCAATAATGTGGAGGCAACTCTTTTGCTGTTGAAGAATGGGGCCAACCGGGATATGCAGGA CAACAAGGAAGAGACACCTTTGTTTCTTGCTGCCCGAGAGGGAAGTTATGAAGCAGCCAAAATCCTGTTA GACCACTTTGCCAACCGGGACATCACGGACCACATGGACCGCCTTCCCCGGGATGTGGCTCGGGACCGCA TGCACCATGACATAGTTCGCCTCCTGGACGAGTACAACGTGACTCCCAGCCCTCCGGGGACTGTCTTGAC TTCTGCTCTCTCACCTGTCCTCTGTGGGCCCAACAGGTCTTTCCTCAGTCTGAAGCACACCCCAATGGGT AAGAAGGCCAGACGGCCCAACACCAAGAGCACCATGCCCACAAGCCTGCCTAACCTTGCCAAGGAGGCCA AGGACGTCAAGGGGAGCAGGAGGAAGAAGTGTCTCAACGAGAAGGTCCAGCTCTCCGAGAGCTCAGTGAC TTTATCCCCTGTCGACTCGCTCGAGTCTCCTCACACATATGTTTCTGATGCCACGTCTTCCCCCATGATC ACGTCCCCTGGGATCTTACAGGCCTCACCCACGCCTCTTCTGGCTGCTGCCCCAGCTGCCCCAGTGCATG CTCAGCATGCACTGTCTTTCTCTAACCTTCACGAAATGCAGCCTTTGGCTCCTGGAGCCAGCACCGTGCT CCCCTCAGTTAGCCAGTTGTTGTCCCACCACCACATCGTCCCCCCAGGGAGTGGCAGTGCAGGAAGCTTG GGCAGGTTACATTCAGTCCCTGTCCCCTCAGACTGGATGAACCGCGTGGAGATGAGTGAGACCCAGTACA GTGAAATGTTTGGCATGGTCCTGGCTCCTGCAGAGGGAACCCACCCTGGCATGGCAGCTCCTCAGAGCAG AGCTCCGGAAGGGAAGCCCATCCCCACACAGCGTGAGCCCTTGCCTCCCATTGTAACTTTCCAGCTCATC CCGAAAGGCAGCCTTGCTCAAGCAGCTGGGGCTCCCCAGACACAGTCCGGCTGCCCCCCAGCTGTGGCAG GCCCCTTGCCCTCTATGTACCAGATCCCAGAGATGGCCCGTTTGCCCAGTGTGGCTTTCCCACCTACCAT GATGCCCCAGCAGGAGGGGCAGGTAGCTCAGACCATTGTCCCAACCTATCACCCTTTCCCAGCCTCTGTG GGCAAGTACCCCACACCCCCTTCCCAACACAGTTATGCCTCCTCAAATGCTGCCGAGCGCACCCCCAATC ATGGTGGTCACCTCCAGGGCGAGCACCCGTACCTGACACCATCCCCAGAGTCTCCTGACCAATGGTCGAG CTCTTCACCGCACTCTGCGTCTGACTGGTCAGATGTGACCACCAGCCCAACTCCTGGAGGGGGTGGAGGC GGTCAGCGGGGACCTGGAACACACATGTCCGAGCCACCACACAGCAACATGCAGGTTTATGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207443 representing NM_024358 Red=Cloning site Green=Tags(s) MPALRPAALRALLWLWLCGAGPAHALQCRGGQEPCVNEGTCVTYHNGTGYCRCPEGFLGEYCQHRDPCEK NRCQNGGTCVTQAMLGKATCRCAPGFTGEDCQYSTSHPCFVSRPCQNGGTCHMLSWDTYECTCQVGFTGK QCQWTDVCLSHPCENGSTCSSVANQFSCRCPAGITGQKCDADINECDIPGRCQHGGTCLNLPGSYRCQCP QGFTGQHCDSPYVPCAPSPCVNGGTCRQTGDFTSECHCLPGFEGSNCERNIDDCPNHKCQNGGVCVDGVN TYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCTNRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPG STCIDRVASFSCLCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECA MANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVH CELEVNECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCAT GFTGTLCDENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQ CNCQPGTSGLNCEINFDDCASNPCLHGACVDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCIND VNGFRCMCPEGPHHPSCYSQVNECLSSPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGG TCNNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCLDDVSGYTCHCMLPYTGKNCQTVLAPCSPN PCENAAVCKEAPNFESFTCLCAPGWQGQRCTVDVDECVSKPCMNNGICHNTQGSYMCECPPGFSGMDCEE DINDCLANPCQNGGSCVDKVNTFSCLCLPGFVGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCTCPAGFH GVHCENNIDECTESSCFNGGTCVDGINSFSCLCPVGFTGPFCLHDINECSSNPCLNSGTCVDGLGTYRCT CPLGYTGKNCQTLVNLCSPSPCKNKGTCAQEKARPRCLCPPGWDGAYCDVLNVSCKAAALQKGVPVEHLC QHSGICINAGNTHHCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDE CQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCAGGPHCLNGGQCVDRIGGYSCRCLPGFAGER CEGDINECLSNPCSSEGSLDCIQLKNNYQCVCRSAFTGRHCETFLDVCPQKPCLNGGTCAVASNVPDGFI CRCPPGFSGARCQSSCGQVKCRRGEQCVHTASGPHCFCLNRKDCESGCASNPCQHGGTCYPQRQPPYYSC RCSPPFWGSHCESYTAPTSTPPATCLSQYCADKARDGICDEACNSHACQWDGGDCSLTMEDPWANCTSSL RCWEYINNQCDELCNTAECLFDNFECQRNSKTCKYDKYCADHFKDNHCDKGCNNEECGWDGLDCAADQPE NLAEGILVIVVLLPPEQLLQDSRSFLRALGTLLHTNLRIKQDSQGALMVYPYYGEKSAAMKKQKVARRSL PDEQEQEIIGSKVFLEIDNRQCVQDSDQCFKNTDAAAALLASHAIQGTLSYPLVSVVSESEDPRNTPLLY LLAVAVVIILFLILLGVIMAKRKRKHGFLWLPEGFTLRRDSSNHKRREPVGQDAVGLKNLSVQVSEANLI GSTTSEHWGDDEGPQPKKAKAEDDEALLSEDDPVDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLD VNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRA DAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMV AELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILL DHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVLCGPNRSFLSLKHTPMG KKARRPNTKSTMPTSLPNLAKEAKDVKGSRRKKCLNEKVQLSESSVTLSPVDSLESPHTYVSDATSSPMI TSPGILQASPTPLLAAAPAAPVHAQHALSFSNLHEMQPLAPGASTVLPSVSQLLSHHHIVPPGSGSAGSL GRLHSVPVPSDWMNRVEMSETQYSEMFGMVLAPAEGTHPGMAAPQSRAPEGKPIPTQREPLPPIVTFQLI PKGSLAQAAGAPQTQSGCPPAVAGPLPSMYQIPEMARLPSVAFPPTMMPQQEGQVAQTIVPTYHPFPASV GKYPTPPSQHSYASSNAAERTPNHGGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGGGG GQRGPGTHMSEPPHSNMQVYA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_024358
ORF Size	7413 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_024358.2, NP_077334.2
RefSeq Size	10723 bp
RefSeq ORF	7416 bp
Locus ID	29492
MW	265.1 kDa
Gene Summary	transmembrane protein that may be involved in cellular interactions that specify cell fate [RGD, Feb 2006]

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