Timeless (NM_031340) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Tim
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207370 representing NM_031340 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTGTACATGATGAACTGTGAACTTCTAGCCACTTGTAGCGCCCTTGGGTACTTGGAAGGAGGCA CTTACCACAAGGAGCCGGACTGCTTGGAGAGTGTGAAGGATTTGATCCGATACCTGAGGCATGAGGACGA GACTCGAGATGTGCGGCAGCAGCTGGGAGCCGCGCAGATCCTGCAGAGTGATCTCCTGCCAATCCTCACC CAGCACCGCCAGGACAAGCCTCTCTTTGATGCTGTGATCAGGCTGATGGTAAATTTGACTCAGCCAGCCT TGCTCTGTTTTGGCAGCGTGCCTAAGGACCCCACTGTACGACACCATTTTCTGCAGGTTCTAACCTACCT GCAGGCCTACAAAGAGGCCTTTGCCAGTGAAAAGGCATTTGGAGTCCTCAGCGAAACCTTGTATGAGCTA CTGCAGCTGGGCTGGGAGGATCGGCAAGAAGAAGACAACTTGCTGATTGAACGGATCCTTCTGTTGGTCA GAAATATTCTCCATGTCCCAGCCAACCTTGAGCAGGAGAAGAGGATCGATGACGATGCCAGCATTCATGA CCGGCTTCTGTGGGCAATTCACCTCAGTGGCATGGACGACTTGCTCCTCTTCCTGTCCAGCTCATCCGCT GAGCAGCAGTGGAGCCTCCATGTGCTGGAGATCATCTCCCTCATGTTCCGAGACCAGAAGCCTGAGCAGC TGGCAGGAGTAGGGCAGGGACGCTTGGCTCAGGAGCGAAGCACAGATTTGGCAGAATTGGAAGTGCTGCG CCAACGGGAGGTGGCCGAGAAGAGAGCCCGAGCCCTGCAGCGAGGAAACAGGCACTCTCGATTTGGGGGC TCCTATGTTGTCCAGGGGTTGAAATCTATTGGGGAGAGGGATGTCGTCTTTCACAAAGGCCTTCACAACC TCCAGAACTACAGCTCAGATCTAGGAAAGCAGCCCCGGAGGGTGCCTAAGCGTCGTCAGGCTGCCCAGGA GCTGTCTGTCCATCGCCGCTCTGTCCTGAATGTGAGGCTCTTCCTCAGAGACTTCTGCTCTGAGTTCCTG GAGAACTGTTACAACCCACTCATGGGTGCAGTCAAGGATCACCTGCTTCGGGAGAAAGCTCAGCAACATG ATGAGACGTACTACATGTGGGCGATGGCTTTCTTTATGGCCTTCAACCGAGCTGCTGCCTTCCGCCCTGG CTTTGTCTCTGAGACCCTCAGTATCCGGACCTTTCACTTTGTGGAGCAGAACCTCACCAACTACTATGAG ATGATGCTGACAGACCGCAAGGAGGCCGCCTCCTGGGCACGCAGGATGCACCTGGCTCTGAAGGCCTATC AGGAGCTGCTGGCCACAGTGAATGAGATGGACATGTGCCCAGACGACGCTGTCAGGGAGAGCAGTCGCAT CATCAAAAACAACATTTTCTACATGATGGAGTACCGAGAACTATTTCTGGCACTCTTTCGAAAATTCGAT GAGAGATACCATCCACGTTCATTCCTTTGTGACCTGGTGGAGACCACTCACCTCTTCCTCAAAATGTTGG AGCGCTTCTGTCGGAGCCGAGGGAACCTGATGGTGCAGAACAAAAGAAAGAAGAGAAAAAAGAAAAAGAA GGCCCAGGAGCAGGGTGTTGCTTTCTCACGTAGCCCTGAGGAGCTGCAGGCCATGTGGTCAGCGCTGGCA GAGCGGCTGCTGCAGTGCGCCCAGGAACCTGAGCTCAGTGTGGACTCCATCATTCCCTTTGATGCGGCCT CAGAGGTGCCAGTGGAGGAGCAGCGGGTAGAAGCTATGGTGAGGATCCAAGACTGCCTTGTGGCTGGCCA GGCCCCGCAAGCCTTGGCCCTCCTGCGGTCTGCCCGGGAAGTGTGGCCTGAAGGAAATGTGTTTGGCTCT CCAGTCATTTCCCCAGGGGAAGAAATGCAGTTGCTAAAACAAATCCTCTCTGCAACCCTTCCCCGGCAGC AAGAGCCAGTAGAAGGAGATGCAGAGGAGGAAGATGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGTTACA GGTGGTCCAGGTGTCAGAGAAGGAGTTTAAGTTTTTGGACTACTTGAAACGCTTTGCATGCTCAACCATT GTGCGAGCCTATGTGCTTCTCCTGCGGAGCTACCGGCAGAACAGCGCTCACACCAACCACTGCATCGCCA AGATGCTGCACCGGCTGGCCCATGACCTGGGGATGGAAGCCCTGCTTTTCCAGCTCTCCCTGTTCTGCCT CTTCAACCAGCTACTCAGTGACCCAGCCGCTGCAGCCTACAAAGAGCTAGTGACTTTTGCCAAATACATC CTCGGCAAGTTCTTTGCGTTGGCTGCAGTGAACCAGAAAGCATTTGTGGAGCTGCTGTTCTGGAAGAACA CCTCGGTGGTTCGGGAAATGACCCAGGGATATGGCTCCCTTGACAGTGGGTCTTGCAGCCACAGAGCCCC TGTGTGGAGCTCTGAGGAAGAGGCCCAGCTTCAAGAACTATACCTCGCCCACAAGGATGTAGAAGGTCAG GATGTAGTGGAGACCATCTTGGCACACCTGAAAGCTGTCCCTCGGACACGCAAGCAGGTCATCCACCATC TGGTACGCATGGGCCTGGCCGACAGTGTCAAGGACTTCCAGAGGAAAGGGACCCAGATTGTCTTGTGGAC AGAGGATCAGGAGCTGGAGTTACAGCGGCTCTTTGAGGAGTTCCAGGATTCCGATGATGTTCTTGGTCAT ATCATGAAGAATATCACAGCCAAACGTTCACGGGCTCGAGTAGTGGACAAACTGTTGGCCCTGGGACTCG TGTCCGAGCGCAGGCAACTGCACAAGAAACGGAGGAAGAAGCTGGCGCCCTCTTGCATGCAGAATGGGGA AGAGTCCCAGAGAGATGCTTGGCAGGAAGATCCAGAAGAAGAAGAGGAAAAAGAAGGCTTGCCAGAGAGT GAGGGTGAGGAGAACGAGGAAGACTTGTTAGCAGGACAGGTCCAGGGCAGCTCATCTCTTTCTGCTGAAA ACCTCCGTCAGAGCCTTTGTCAGGACGGCCTCTCTGCTCCCCTCCTGTGGCTCCAGAGCTCCCTGATTCG CGCAGCAGATGACCGAGAAGAGGATGGCTGCTCCCAGGCCATCCCTCTGGTGCCTCTGACGGAAGAAAAT GAGGAAGCAATGGAGAACAAACAGTTTCAGCATCTGCTACGTAAGCTAGGAATCCGGGCCCCCTGCTCAG GACAGGAAACCTTCTGGCGAATTCCAGCCAAACTGAGCTCCACCCAGCTTCGGAGAGTGGCTGCTTCTCT GAGTGAGCGAGAAAACAAGGAGGAAAGAGAGGAGGAGCCAGAGCCGAATCCAGGAGTCCCTGGAGAGCAG AGTCCCAGTGAGGAGCACCAAGTGAGAGCCCCGAGAGCCCTTTTGTCAGCTCGTAAAAGGAAAGCAGGCC TGGTGTTTCCAAAAGAGGAGGCCACTGGGGAGGAAGAATGGAAATCGGTGCCTAAGAAGCAGCAACTGCT GGACAGCGACGAAGAGGACACCGACGACGAGAGGGGAGGGCAAGCAGCAGTGTCGGGAACCCTGAGAATC CACAAGGAGAAACGGTTTCTGGTTGAGGATGAAGATGAGGATTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207370 representing NM_031340 Red=Cloning site Green=Tags(s) MDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILT QHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDPTVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYEL LQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKRIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSA EQQWSLHVLEIISLMFRDQKPEQLAGVGQGRLAQERSTDLAELEVLRQREVAEKRARALQRGNRHSRFGG SYVVQGLKSIGERDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFL ENCYNPLMGAVKDHLLREKAQQHDETYYMWAMAFFMAFNRAAAFRPGFVSETLSIRTFHFVEQNLTNYYE MMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDDAVRESSRIIKNNIFYMMEYRELFLALFRKFD ERYHPRSFLCDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKAQEQGVAFSRSPEELQAMWSALA ERLLQCAQEPELSVDSIIPFDAASEVPVEEQRVEAMVRIQDCLVAGQAPQALALLRSAREVWPEGNVFGS PVISPGEEMQLLKQILSATLPRQQEPVEGDAEEEDEEEEEEEEEELQVVQVSEKEFKFLDYLKRFACSTI VRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHDLGMEALLFQLSLFCLFNQLLSDPAAAAYKELVTFAKYI LGKFFALAAVNQKAFVELLFWKNTSVVREMTQGYGSLDSGSCSHRAPVWSSEEEAQLQELYLAHKDVEGQ DVVETILAHLKAVPRTRKQVIHHLVRMGLADSVKDFQRKGTQIVLWTEDQELELQRLFEEFQDSDDVLGH IMKNITAKRSRARVVDKLLALGLVSERRQLHKKRRKKLAPSCMQNGEESQRDAWQEDPEEEEEKEGLPES EGEENEEDLLAGQVQGSSSLSAENLRQSLCQDGLSAPLLWLQSSLIRAADDREEDGCSQAIPLVPLTEEN EEAMENKQFQHLLRKLGIRAPCSGQETFWRIPAKLSSTQLRRVAASLSERENKEEREEEPEPNPGVPGEQ SPSEEHQVRAPRALLSARKRKAGLVFPKEEATGEEEWKSVPKKQQLLDSDEEDTDDERGGQAAVSGTLRI HKEKRFLVEDEDEDY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031340
ORF Size	3615 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031340.1, NP_112630.1
RefSeq Size	4237 bp
RefSeq ORF	3618 bp
Locus ID	83508
UniProt ID	Q9Z2Y1
MW	138.6 kDa
Gene Summary	The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the Period genes (Per1, Per2, and Per3) and others to downregulate activation of Per1 by Clock/Arntl. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. [provided by RefSeq, Feb 2014]

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