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Smad4 (NM_019275) Rat Tagged ORF Clone
CAT＃: RR205918
- TrueORF®
Smad4 (Myc-DDK-tagged ORF) - Rat SMAD family member 4 (Smad4), (10 ug)
"NM_019275" in other vectors (3)
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CNY 7,030.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Madh4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205918 representing NM_019275
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAATATGTCTATTACAAATACACCGACAAGCAATGACGCCTGTCTGAGCATTGTACACAGTTTGA TGTGCCACAGACAAGGTGGAGAGAGTGAGACATTTGCCAAAAGAGCAATTGAGAGTCTGGTAAAGAAGCT GAAAGAGAAGAAAGATGAACTGGATTCTTTAATAACAGCTATCACTACAAATGGAGCTCACCCCAGCAAG TGTGTCACCATACAGAGAACACTGGATGGACGACTGCAGGTGGCTGGTCGGAAAGGATTTCCTCATGTGA TCTATGCCCGTCTGTGGAGGTGGCCTGATCTACACAAGAACGAACTAAAACATGTTAAATATTGTCAGTA TGCATTTGACTTAAAATGTGATAGCGTCTGTGTGAACCCATATCACTATGAGCGGGTTGTCTCACCTGGA ATTGATCTCTCTGGATTAACACTGCAGAGTAATGCTCCACCAAGTATGTTAGTGAAGGATGAATATGTTC ATGACTTTGAAGGACAGCCATCCTTACCCACGGAAGGACATTCGATTCAAACCATCCAACATCCACCAAG TAATCGCGCATCCACGGAGACGTACAGCGCCCCAGCTCTGTTAGCCCCATCAGAGTCTAATGCCACCAGT ACCACCAACTTCCCCAACATTCCTGTGGCTTCCACAAGTCAGCCGGCCAGCATTCTGGCAGGCAGCCATA GTGAAGGACTGTTGCAGATAGCTTCAGGGCCTCAGCCAGGACAGCAGCAGAATGGATTCACTGCTCAGCC AGCTACTTACCACCATAACAGCACTACCACCTGGACTGGAAGTAGGACTGCACCATACACACCTAATTTG CCTCACCACCAAAACGGCCATCTTCAGCACCACCCGCCTATGCCGCCCCATCCTGGACATTACTGGCCGG TTCACAATGAGCTTGCATTCCAGCCTCCCATTTCCAATCATCCTGCTCCTGAATATTGGTGTTCCATTGC TTACTTTGAAATGGATGTTCAGGTAGGAGAGACATTTAAAGTCCCTTCAAGCTGTCCTATTGTAACTGTG GATGGATACGTGGATCCTTCTGGAGGAGATCGCTTTTGCTTGGGTCAACTCTCCAATGTCCACAGGACAG AAGCTATTGAGAGAGCAAGGTTGCACATAGGCAAAGGAGTGCAGTTGGAGTGTAAAGGTGAAGGGGACGT GTGGGTCAGGTGCCTCAGTGACCACGCCGTCTTCGTGCAGAGTTACTACCTGGACCGTGAAGCTGGCCGA GCACCTGGTGACGCCGTCCACAAGATCTACCCGAGTGCGTATATAAAGGTCTTTGATTTGCGGCAGTGTC ACCGGCAGATGCAGCAGCAGGCGGCCACCGCGCAAGCCGCAGCTGCTGCCCAGGCGGCAGCTGTGGCAGG AAACATCCCTGGTCCTGGGTCCGTAGGTGGAATAGCCCCGGCCATCAGTCTGTCTGCTGCTGCTGGCATT GGTGTAGACGACCTCCGGCGCTTGTGCATACTCAGGATGAGCTTCGTGAAGGGCTGGGGCCCAGACTACC CGAGACAGAGCATCAAAGAAACCCCGTGCTGGATTGAGATTCACCTCCACCGAGCTCTGCAGCTCCTGGA TGAAGTCCTGCACACCATGCCCATTGCGGACCCACAGCCTTTAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205918 representing NM_019275
Red=Cloning site Green=Tags(s) MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSK CVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPG IDLSGLTLQSNAPPSMLVKDEYVHDFEGQPSLPTEGHSIQTIQHPPSNRASTETYSAPALLAPSESNATS TTNFPNIPVASTSQPASILAGSHSEGLLQIASGPQPGQQQNGFTAQPATYHHNSTTTWTGSRTAPYTPNL PHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTV DGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGR APGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGI GVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019275 |
| ORF Size | 1656 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019275.3, NP_062148.1 |
| RefSeq Size | 3056 bp |
| RefSeq ORF | 1659 bp |
| Locus ID | 50554 |
| UniProt ID | O70437 |
| MW | 60.5 kDa |
| Gene Summary | transduces signal from TGF-beta; deletion of human homolog gene is associated with pancreatic carcinomas [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205918 | Smad4 (untagged ORF) - Rat SMAD family member 4 (Smad4), (10 ug) |
CNY 6,176.00 |
|
| RR205918L3 | Lenti ORF clone of Smad4 (Myc-DDK-tagged ORF) - Rat SMAD family member 4 (Smad4), (10 ug) |
CNY 9,030.00 |
|
| RR205918L4 | Lenti ORF clone of Smad4 (mGFP-tagged ORF) - Rat SMAD family member 4 (Smad4), (10 ug) |
CNY 9,880.00 |
