Hdac8 (NM_001126373) Rat Tagged ORF Clone

CAT#: RR205688

  • TrueORF®

Hdac8 (Myc-DDK-tagged ORF) - Rat histone deacetylase 8 (Hdac8), (10 ug)



  "NM_001126373" in other vectors (3)

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CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms HD8; RGD1562895
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR205688 representing NM_001126373
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATACCAGAGGAACCCGCCAATAGTGGGCATTCGCTACCCCCGGTTTATATTTACAGTCCCGAGT
ATGTCAGCATCTGCGATTCCCTTGTGAAGGTCCCCAAACGGGCCAGTATGGTTCACTCTCTGATCGAAGC
ATATGCCCTGCATAAACAAATGAGGATAGTGAAGCCCAAAGTGGCCTCCATGGAGGAGATGGCCACCTTC
CACACTGATGCCTATCTTCAACATCTCCAGAAGGTCAGCCAAGAAGGGGATGAGGACCATCCAGACTCCA
TAGAATATGGACTAGGTTATGACTGCCCAGCCACAGAAGGGATATTTGACTATGCAGCAGCTATAGGAGG
AGGTACAATCACAGCTGCCCAGTGCCTGATTGACGGGAAGTGTAAAGTAGCCATTAACTGGTCTGGAGGG
TGGCATCATGCAAAGAAAGATGAAGCATCTGGTTTCTGTTATCTCAATGATGCTGTCCTAGGAATATTAC
GATTGCGACGGAAATTTGACCGGATTCTCTATGTGGACTTGGATCTACACCATGGAGATGGTGTAGAAGA
TGCCTTCAGTTTTACATCTAAAGTTATGACTGTGTCCCTGCACAAGTTCTCTCCAGGATTTTTCCCAGGA
ACAGGTGACATGTCTGATGTTGGCCTGGGGAAAGGACGGTACTACAGTGTCAATGTGCCCATTCAGGATG
GCATCCAGGATGAGAAGTACTATCACATCTGTGAAAGTGTACTAAAGGAAGTATACCAGGCCTTCAATCC
GAAGGCAGTGGTTCTACAGCTGGGAGCAGATACCATTGCTGGAGATCCAATGTGCTCCTTTAACATGACA
CCAGTGGGAATTGGCAAGTGTCTGAAGTATGTCCTTCAGTGGCAGTTGGCAACTCTGATTTTAGGAGGAG
GAGGCTACAACCTTGCCAACACGGCTCGTTGCTGGACATACTTGACCGGGGTCATCCTAGGGAAAACACT
ATCCTCTGAGATCCCAGATCATGAGTTTTTCACAGCATATGGTCCTGATTATGTGCTGGAAATCACGCCA
AGCTGCCGGCCAGACCGCAATGAGCCCCATCGAATCCAGCAAATCCTCAACTACATCAAAGGGAATCTGA
AGCATGTGGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR205688 representing NM_001126373
Red=Cloning site Green=Tags(s)

MEIPEEPANSGHSLPPVYIYSPEYVSICDSLVKVPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATF
HTDAYLQHLQKVSQEGDEDHPDSIEYGLGYDCPATEGIFDYAAAIGGGTITAAQCLIDGKCKVAINWSGG
WHHAKKDEASGFCYLNDAVLGILRLRRKFDRILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPG
TGDMSDVGLGKGRYYSVNVPIQDGIQDEKYYHICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMT
PVGIGKCLKYVLQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITP
SCRPDRNEPHRIQQILNYIKGNLKHVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001126373
ORF Size 1131 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001126373.2, NP_001119845.2
RefSeq Size 2002 bp
RefSeq ORF 1134 bp
Locus ID 363481
UniProt ID B1WC68
MW 41.8 kDa
Gene Summary Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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