Pik3c2a (NM_001108500) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205077 representing NM_001108500 Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGCTCAGATTTCCAACAACAGTGAATTTAAACAGTGTTCATCTTCACATCCAGAACCAATAAGAACCA AAGATGTGGACAAAGCAGAAGCATTACAGATGGAAGCAGAAGCCTTAGCAAAATTGCAGAAGGATAGACA CATGACTGACAGTCCAAGAGGCTTTGAGCTGTCTAACAGCACTAGACAAAGAACACAGGGTTTTAACAAA CAGGATTATGATCTCATGGTGTTTCCTGAGTCGGATTCCCAAAAAAGAGCATTAGATATTGACGTAGAAA AGCTTACCCAAGCTGAACTTGAAAAGATATTGCTGGATGACAATTTTGAAACTAGAAAAACTCCTGCATC GCCAGTTACTCCTGTTCTGAGCCCTTCATTTTCAGCACAGCTATATCTTAGACCTGCTGTTCAAAGAAGC CAGTGGCCCCCTGGATTATGCGGGCCTTCCACATACACTTTACCTTCTATTTATCCTTCAGCATACAGTA AACAGGCCACATTCCAGAATGGCTTCAGTCCAAGGATGCCTACTTTTCCATCTACAGAGTCTATATATTT AAGACTTCCAGGACAGTCTCCATATTTTTCATATCCTTTGACACCTGCCACACCATTTCATCCACAAGGA AGTTTACCAGTCTATCGGCCACTAGTCAGTCCTGACATGGCAAAACTATTTGACAAAATAGCAAGTACCT CAGAATTTTTAAAAAATGGGAAAGCAAGGACTGATTTGGAGATAGCAAACTCGAAAGCTTCAGTCTGCAA TCTACAAATATCTCCAAAGTCTGAAGACATCAATAAATTTGATTGGTTGGACTTGGATCCTCTGAGTAAG CCTAAGATAGACTGTGTGGAAGTGTTAGAACATGAAGAAGAGAAGAAGAATCCAGTTTTGCTAGCAGAGG ATCCTTGGGATGCTGTTCTTCTTGAAGAGAGATCACCAACAAGTCGTCACCTAGAAAGGAAGGTGAATGG AAAATCCCTTTCTGGGGCGACTGTAACAAGAAGCCAGTCTTTAATCGTTCGGACAACTCAAGTTACAAAA GCCCAGAGCCAGGTATCTCAGAAAGACCCAAATGGGACCAGTAGTTTGGCAACTGGAAGTTCTCTTCTAC AAGAATTTGAAGTACAGAATGAGGAGGTGGCAGCTTTTTGTCAATCCATTATGAAATTAAAGACCAAATT TCCATATACCGATCACTGTACAAATCCAGGCTATTTGTTAAGTCCAGTCACAGTGCAAAGAAACATGTGT GGGGAAAATGCCAGTGTGAAGGTCTCCATTGAAATTGAAGGGTTTCAACAACCAGTTACTTTTACATGTG ATGTGAGTTCTACTGTAGACATAATTATAATGCAAGCCCTTTGCTGGGTACATGATGACTTGAATCAAGT GGATGTTGGCAGCTACATTCTGAAAGTTTGTGGTCAAGAGGAGGTTCTACAGAATAATCATTGCCTTGGA AGTCACGAACATATTCAAAATTGTCGAAAATGGGACACAGAGATTAAATTACAACTCTTGACCTTCAGTG CAATGTGCCAGAATCTGGCTCGAACAGCAGAAGATGATGAAGCACCTGTGGATTTAAACAAGTACTTGTA TCAAATAGAAAAACCTTATAAAGAAGTCATGACAAGACACCCTGTTGAAGAACTCTTAGATTCCTATCAC TACCAAGTAGAACTGGCTCTACAAACTGAAAACCAGCACCGAGCTATTGATCAAGTGATTAAAGCAGTAA GAAAAATCTGTAGTGCTTTAGATGGGGTTGAGACCCCCGCCGTCACAGAAGCAGTGAAGAAGCTAAAGCG AGCAGTTAACCTTCCAAGGAACAAAAGTGCTGATGTGACTTCATTATCTGGAGGAGATACTAGCAAGAAC TCAACTAGGGGCTCACTGAATCTTGAAAATCCTGTTCAAGTAAGCATGGATCACTTAACGGCAGCAATTT ATGATCTTCTCAGGCTTCATACAAATTCTTGTAAGAGTTCTACAGTCTGTCCCCAAGGTAGCAGGAACAT CAAGGAAGCATGGACTGCAACAGAGCAGCTCCAGTTCACCGTCTATGCCGCACACGGAATTTCCAGTAAC TGGGTATCCAATTACGAAAAATACTACTTGATATGTTCCCTGTCTCACAATGGGAAGGATCTTTTTAAGC CTATTCAGTCAAAGAAGGTTGGCACATACAAGAATTTCTTCTATCTTATCAAATGGGATGAACTAATCAT TTTCCCTATCCAGATATCGCAGTTGCCTTTAGAGTCAGTTCTTCATCTCACTTTGTTTGGAGTTTTAAAT CAGAGCAGTGGAAGTTCCCCTGATTCTAATAAACAGAGAAAGGGGCCAGAAGCTCTGGGCAAAGTCTCTT TAACTCTATTTGACTTTAAACGGTTTTTAACATGTGGAACTAAACTTCTCTACCTTTGGACTTCATCACA TACAAATTCTATTCCTGGAGCAATCCCCCAAAAAAGCTATGTCATGGAAAGAATTGTGCTACAGGTTGAT TTTCCTTCTCCTGCATTTGACATTATTTATACATCTCCTCAAATTGATAGAAACATTATACAGCAATACA AGCTGGAAGCACTGGAGAATGAAATAAAGGGAAAACTTCTGGATATTGTTCACAGAGATTCATCATTTGG ACTTTCTAAAGAAGATAAGGCCTTTTTGTGGGAAAAACGCTATTATTGCCTAAAACATCCAAATTGTCTT CCGAAGATACTAGCAAGTGCTCCAAACTGGAAGTGGGCTAATCTTGCCAAAACTTACTCTTTGCTCCACC AGTGGCCGCCATTGTGTCCATTAGCTGCATTGGAGCTCCTTGATGCAAAATTTGCTGATCAGGAAGTGCG ATCGCTAGCTGTGAGCTGGATTGAGGCCATTAGTGATGATGAGCTAACAGATCTTCTCCCACAGTTTGTA CAGGCTTTGAAATATGAAATTTATTTGAATAGTTCACTAGTGCGCTTCCTTCTGTCCAGGGCACTGGGAA ACATCCAGATAGCACACAGTTTGTATTGGCTTCTCAAGGATGCTTTGCACGATACACGGTTTGGAAGCAG ATATGAACATGTGTTGGGTGCTCTCCTCTCTGTAGGAGGAAAAGGACTCAGAGAAGAGCTTTCTAAGCAG ATGAAACTTGTACAGCTTTTAGGAGGAGTGGCAGAAAAAGTAAGGCAGGCTAGTGGATCAGCCAGACAGG TGGTCCTCCAGAAGAGTATGGAACGAGTACAGTCCTTTTTTCTGAGAAATAAATGCCGGGTTCCTCTCAA ACCAAGTCTAGTAGCGAAAGAATTAAATATTAAGTCATGCTCATTCTTCAGTTCGAATGCCATGCCTCTG AAAGTCACAATGGTGAATGCTGATCCTCTGGGAGAAGAAATTAATGTCATGTTTAAGGTTGGTGAAGATC TTCGGCAAGATATGTTAGCTTTACAGATGATAAAGATTATGGATAAGATCTGGCTTAAAGAGGGCCTGGA TCTGAGGATGGTGATATTCAAATGCCTGTCGACTGGCCGAGATCAAGGCATGGTGGAGCTAGTTCCTGCC TCAGACACTCTCAGGAAAATCCAAGTGGAATATGGTGTGACAGGATCCTTTAAAGATAAACCACTTGCTG AATGGCTAAGGAAATACAACCCTTCTGAAGAAGAATATGAAAAGGCTTCTGAGAACTTTATCTACTCTTG TGCTGGGTGCTGTGTAGCCACTTATGTTTTAGGCATTTGTGATCGGCACAATGACAATATAATGCTTCGA AGCACAGGACACATGTTCCACATTGACTTTGGAAAGTTTTTGGGCCATGCACAGATGTTTGGTAGCTTCA AAAGGGACCGCGCTCCTTTCGTGCTTACCTCTGACATGGCCTATGTCATTAATGGAGGTGAAAAGCCCAC CATTCGTTTCCAGTTGTTTGTGGACCTCTGCTGTCAAGCCTACAACTTGATAAGAAAGCAAACAAACCTC TTTCTTAACCTTCTCTCACTGATGATTCCTTCAGGATTGCCAGAACTTACAAGTATTCAGGATTTGAAAT ATGTTAGAGATGCACTTCAGCCCCAAACTACAGATGCTGAAGCTACTATTTTCTTTACTAGGCTGATTGA GTCAAGTTTGGGAAGCATTGCCACAAAGTTTAATTTCTTCATTCATAACCTTGCTCAGCTACGTTTTTCT GGCCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCAAAAACATACTCCTTTAGACAAGACGGCC GGATCAAAGAAGTCTCTGTTTTCACATATCATAAGAAATACAACCCAGATAAACACTATATTTATGTGGT TCGAATTCTGAGGGAAGGACACCTTGAACCATCATTTGTATTCCGGACATTTGATGAGTTTCAGGAACTG CACAATAAGCTCAGCATTATTTTCCCACTTTGGAAGTTACCTGGCTTTCCTAATAGGATGGTTCTAGGAA GAACACACATCAAAGATGTAGCAGCCAAGAGGAAAATTGAATTAAACAGTTATTTACAGAGTTTGATGAA TGCATCAACAGATGTAGCAGAGTGTGACCTTGTTTGTACTTTTTTCCACCCCTTACTTCGTGATGAAAAA GCTGAAGGAATAGCTAGGTCTACAGGTACAAGTCCCTTCAGCCCTACTTTGGGCCAAATAGGAGGAGCAG TGAAGTTATCGGTTTCTTACCGAAATGGCACCCTCTTCATCATGGTGATGCACATCAAAGATCTGGTGAC TGAAGATGGGGCTGACCCAAATCCATACGTCAAAACATACCTGCTTCCAGATACCCACAAAACGTCAAAA CGTAAAACCAAAATTTCACGTAAAACTAGGAACCCAACATTCAATGAAATGCTTGTATATAGTGGATACA GCAGAGAAACTCTGAGACAGAGAGAACTTCAACTGAGTGTGCTCAGTGCAGAATCTCTGCGGGAGAATTT CTTCCTGGGTGGAATAACCCTGCCACTGAAAGATTTCAACTTGAGCAAAGAGACAGTTAAGTGGTATCAG CTGACTGCGGCAACATACCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205077 representing NM_001108500 Red=Cloning site Green=Tags(s) MAQISNNSEFKQCSSSHPEPIRTKDVDKAEALQMEAEALAKLQKDRHMTDSPRGFELSNSTRQRTQGFNK QDYDLMVFPESDSQKRALDIDVEKLTQAELEKILLDDNFETRKTPASPVTPVLSPSFSAQLYLRPAVQRS QWPPGLCGPSTYTLPSIYPSAYSKQATFQNGFSPRMPTFPSTESIYLRLPGQSPYFSYPLTPATPFHPQG SLPVYRPLVSPDMAKLFDKIASTSEFLKNGKARTDLEIANSKASVCNLQISPKSEDINKFDWLDLDPLSK PKIDCVEVLEHEEEKKNPVLLAEDPWDAVLLEERSPTSRHLERKVNGKSLSGATVTRSQSLIVRTTQVTK AQSQVSQKDPNGTSSLATGSSLLQEFEVQNEEVAAFCQSIMKLKTKFPYTDHCTNPGYLLSPVTVQRNMC GENASVKVSIEIEGFQQPVTFTCDVSSTVDIIIMQALCWVHDDLNQVDVGSYILKVCGQEEVLQNNHCLG SHEHIQNCRKWDTEIKLQLLTFSAMCQNLARTAEDDEAPVDLNKYLYQIEKPYKEVMTRHPVEELLDSYH YQVELALQTENQHRAIDQVIKAVRKICSALDGVETPAVTEAVKKLKRAVNLPRNKSADVTSLSGGDTSKN STRGSLNLENPVQVSMDHLTAAIYDLLRLHTNSCKSSTVCPQGSRNIKEAWTATEQLQFTVYAAHGISSN WVSNYEKYYLICSLSHNGKDLFKPIQSKKVGTYKNFFYLIKWDELIIFPIQISQLPLESVLHLTLFGVLN QSSGSSPDSNKQRKGPEALGKVSLTLFDFKRFLTCGTKLLYLWTSSHTNSIPGAIPQKSYVMERIVLQVD FPSPAFDIIYTSPQIDRNIIQQYKLEALENEIKGKLLDIVHRDSSFGLSKEDKAFLWEKRYYCLKHPNCL PKILASAPNWKWANLAKTYSLLHQWPPLCPLAALELLDAKFADQEVRSLAVSWIEAISDDELTDLLPQFV QALKYEIYLNSSLVRFLLSRALGNIQIAHSLYWLLKDALHDTRFGSRYEHVLGALLSVGGKGLREELSKQ MKLVQLLGGVAEKVRQASGSARQVVLQKSMERVQSFFLRNKCRVPLKPSLVAKELNIKSCSFFSSNAMPL KVTMVNADPLGEEINVMFKVGEDLRQDMLALQMIKIMDKIWLKEGLDLRMVIFKCLSTGRDQGMVELVPA SDTLRKIQVEYGVTGSFKDKPLAEWLRKYNPSEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLR STGHMFHIDFGKFLGHAQMFGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQAYNLIRKQTNL FLNLLSLMIPSGLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIESSLGSIATKFNFFIHNLAQLRFS GLPSNDEPILSFSPKTYSFRQDGRIKEVSVFTYHKKYNPDKHYIYVVRILREGHLEPSFVFRTFDEFQEL HNKLSIIFPLWKLPGFPNRMVLGRTHIKDVAAKRKIELNSYLQSLMNASTDVAECDLVCTFFHPLLRDEK AEGIARSTGTSPFSPTLGQIGGAVKLSVSYRNGTLFIMVMHIKDLVTEDGADPNPYVKTYLLPDTHKTSK RKTKISRKTRNPTFNEMLVYSGYSRETLRQRELQLSVLSAESLRENFFLGGITLPLKDFNLSKETVKWYQ LTAATYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	AscI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108500
ORF Size	5061 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108500.1, NP_001101970.1
RefSeq Size	8316 bp
RefSeq ORF	5064 bp
Locus ID	361632
MW	190.9 kDa

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