Ptpn21 (NM_133545) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ptp2E
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203694 representing NM_133545 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACTGCCATTTGGGTTGAAATTGAAACGCACCCGGCGCTACACTGTGTCCAGCAAGAGCTGCCTGG TTGCACGGATCCAGCTGCTCAATAATGAGTTTGTGGAGTTCACTCTGTCCGTGGAGAGCACTGGCCAGGA GAGCCTAGAAGCTGTGGCCCAACGCCTGGAGCTGAGAGAGATCACGTACTTCAGCCTCTGGTACTACAAC AAGCAGAATCAGCGTCGCTGGGTGGATTTGGAGAAACCCCTGAAGAAGCAGCTGGACAAGCACGCGCTGG AGCCCACCGTCTACTTTGGAGTGGTGTTTTATGTGCCCTCTGTTTCCCAGCTGCAGCAGGAGATCACCAG GTACCAGTATTACCTGCAGCTGAAGAAGGATGTTCTGGAAGGAAACCTTCCCTGCACATTGGAGCAAGCC ATTCAGCTGGCTGGCTTAGCTGTTCAAGCTGATTTTGGCGACTTTGATCAGTATGAATCCCAAGATTTTC TTCAGAAATTCGCCTTGCTCCCTGTGGGGTGGCTACAGGATGAAAAACTGCTGGAAGAAGCAGCCCAAAA AGTGGCCTTGCTGCATCAGAAATACAGAGGGCTCACAGCTCCTGAGGCCGAGATGCTGTACATGCAGGAG GTAGAGCGGATGGACGGCTATGGAGAGGAGAGCTACCCTGCCAAGGACAGCCAAGGAAGTGACATATCCA TTGGTGCTTGTCTCGATGGCATCTTTGTGAAGCACAAGAATGGGAGGCCTCCCGTGGTGTTCAGGTGGCA CGACATTGCTAACATGTCACACAATAAGTCCTTTTTTGCGTTAGAACTGGCAAATAAAGAGGAGACCATC CAGTTTCAAACTGAAGACATGGAAACAGCAAAGTACGTGTGGAGGCTGTGTGTGGCACGCCACAAATTTT ACAGACTGAACCAGTGTAACCTGCAAACTCAGGCTGCCACGTTGAACTCCGTCAGGAGGGGCTCATCGTC CAGGATGTCTCTGCCTAAACCCCAGCCCTATGCGATGCCTCCCCCACCCCAGCTGCATTATAATGGACAT TATACAGAGCCATTTGCTTCTTCCCAAGACAATGTCTTTGTGCCCAACAAGAATGGATTCTATTGCCACT CCCAGACAAGTTTGGACAGAACCCAAATTGACCTCAGTGGCCGCATCCGCAACGGCAGCGTGTACAGTGC ACACAGTACGAACTCCTTAAACACACCTCAGCCCTACCTGCAGCCCTCCCCTATGTCCTCCAACCCCAGT ATTCCAGGCAGCGATGTCATGAGACCTGACTACATCCCATCCCACCGGCACAGCGCCTTGATTCCCCCAT CCTACCGCCCGACCCCTGACTACGAGAGCGTGATGAAGCGGCTCAACAGAGGCATGGTGCATGCAGACAG GCATAGCCACTCGCTCAGGAACCTCAACATTGGCAGTTCCTATGCATACAGCAGGCCCGACGCCTTGGTC TACAGCCAGCCTGAGATTCGGGAACACCCACACCTCGCCTCTCCCCAGTCAGCCCACTACCCATTTAACT TGAACTACAGTTTCCACAGCCAGGCTCCGTATCCATACCCTGTGGAGAGGCGGCCTGTGGTGGGCGCTGT GAGTGTCCCTGAGCTGACCAATGTGCAGCTGCAGGCCCAGGACTACCCAGCTCCAAACATTATGAGGACC CAGGTGTACCGGCCGCCCCCGCCATATCCTTACCCAAGACCTGCCAACAGCACCCCGGACCTATCGCGGC ACCTCTACATCAGCAGCAGCAATCCAGATCTCATCACCAGACGCGTCCATCACTCGGTGCAGACCTTCCA GGAGGACAGCTTGCCCGTGGCTCACTCTCTGCAGGAGGTGAGTGAGCCCCTCACAGCAGCTCGGCATGCA CACCTACAGAAGAGGAACAGTATTGAAATCGCAGGGCTCACACATGGTTTCGAAGGCCTGAGGCTCAAGG AGGAGACGATGTCAGCCTCAGCCGCAGACGTGGCTCCCAGGACCTTCTCAGCAGGCTCCCAGTCCAGCGT CTTCTCTGACAAAGTGAAGCAGGAGGGAACCGAAGAGCAGGGGAGTGGTGGGTACAGTCACAAGAAGTCC CTTTCTGATGCCACCATGCTGATTCACAGCAGCGAGGAGGATGAGGACTTAGAAGACGACAGCAGCAGAG AACACGCTGTGTCTGAGCCCCGCCTTACGGCTGCTTTCTCTCAGGAACAACAGCTAAACTACCCCTGTGC TTCAGTGACTCCGGTCACTGGCCCTCTGCATATTTTCGAGCCCAAGTCCCATGTTACGGAGCCTGAGAAG AGGGCAAAGGACATCAGCCCTGTCCACCTGGTTATGGAGACTCATCAGCCCCGAAGACATGGACTGCTGA CCCCCTCCATGTCCGAGTCAGACCTCACGACGTCAGGGAGGTACCGAGCTAGGAGGGACTCTCTCAAGAA GAGGCCAGTGTCAGATCTCCTCTCTGGGAAGAAGAACACTGTGGAAGGACTTCCGCCACTAGGGGGAATG AAAAAGACTCGAGCAGATGCAAAAAAAATTGGCCCCCTCAAGCTGGCAGCCCTCAATGGACTCTCCCTGT CCCGGCTGCCCCTGCCTGACGAAGGCAAAGAAGTGTCCACCAGAGCGACAAATGACGAGAGGTGTAAAGT TCTGGAGCAGCGGTTAGAGCAAGGAACGGTCTTCACCGAGTATGAGAGGATTCTGAAAAAGCGGCTGGTT GATGGCGAGTGCTCAACAGCTCGGCTCCCTGAAAATGCAGAGAGAAACCGATTCCAAGATGTGCTCCCCT ACGATGATGCCAGAGTGGAGTTGGTGCCAACCAAGGAGAACAACACTGGCTATATCAATGCGTCACACAT TAAGGTCTCTGTCAGCGGAATTGAATGGGATTATATTGCCACACAGGGACCATTACAAAATACCTGCCAG GACTTTTGGCAGATGGTGTGGGAACAGGGAGTCGCAATTATAGCAATGGTGACAGCGGAAGAGGAGGGAG GTCGGGAGAAGAGCTTTAGGTATTGGCCACGGCTTGGCTCCAGGCACAACACTGTCACCTATGGAAGGTT TAAGATCACAACTCGCTTCCGCACGGACTCGGGCTGCTACGCCACCACAGGTTTGAAGATGAAGCACCTT CTCACAGGGCAGGAGAGGACAGTTTGGCATCTCCAGTACACAGACTGGCCTGAACATGGCTGTCCCGAAG ACCTCAAGGGATTTTTATCATACCTTGAAGAGATCCAGTCAGTTCGACGTCATACAAACAGTACAAGTGA ACCCAGAAGTCCCAACCCCCCGCTGCTGGTGCACTGCAGTGCTGGCGTGGGAAGAACTGGGGTTGTCATT CTGTCAGAGATCATGGTGGCCTGTCTGGAACACAATGAGGTGCTGGACATCCCCAGAGTGCTGGAGCTGC TGAGGCAGCAGAGGATGATGCTGGTGCAGACACTTAGCCAGTACACGTTCGTGTACCGGGTGCTCATTCA GTTCCTGAAGAGCTCTAGGCTCATC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203694 representing NM_133545 Red=Cloning site Green=Tags(s) MPLPFGLKLKRTRRYTVSSKSCLVARIQLLNNEFVEFTLSVESTGQESLEAVAQRLELREITYFSLWYYN KQNQRRWVDLEKPLKKQLDKHALEPTVYFGVVFYVPSVSQLQQEITRYQYYLQLKKDVLEGNLPCTLEQA IQLAGLAVQADFGDFDQYESQDFLQKFALLPVGWLQDEKLLEEAAQKVALLHQKYRGLTAPEAEMLYMQE VERMDGYGEESYPAKDSQGSDISIGACLDGIFVKHKNGRPPVVFRWHDIANMSHNKSFFALELANKEETI QFQTEDMETAKYVWRLCVARHKFYRLNQCNLQTQAATLNSVRRGSSSRMSLPKPQPYAMPPPPQLHYNGH YTEPFASSQDNVFVPNKNGFYCHSQTSLDRTQIDLSGRIRNGSVYSAHSTNSLNTPQPYLQPSPMSSNPS IPGSDVMRPDYIPSHRHSALIPPSYRPTPDYESVMKRLNRGMVHADRHSHSLRNLNIGSSYAYSRPDALV YSQPEIREHPHLASPQSAHYPFNLNYSFHSQAPYPYPVERRPVVGAVSVPELTNVQLQAQDYPAPNIMRT QVYRPPPPYPYPRPANSTPDLSRHLYISSSNPDLITRRVHHSVQTFQEDSLPVAHSLQEVSEPLTAARHA HLQKRNSIEIAGLTHGFEGLRLKEETMSASAADVAPRTFSAGSQSSVFSDKVKQEGTEEQGSGGYSHKKS LSDATMLIHSSEEDEDLEDDSSREHAVSEPRLTAAFSQEQQLNYPCASVTPVTGPLHIFEPKSHVTEPEK RAKDISPVHLVMETHQPRRHGLLTPSMSESDLTTSGRYRARRDSLKKRPVSDLLSGKKNTVEGLPPLGGM KKTRADAKKIGPLKLAALNGLSLSRLPLPDEGKEVSTRATNDERCKVLEQRLEQGTVFTEYERILKKRLV DGECSTARLPENAERNRFQDVLPYDDARVELVPTKENNTGYINASHIKVSVSGIEWDYIATQGPLQNTCQ DFWQMVWEQGVAIIAMVTAEEEGGREKSFRYWPRLGSRHNTVTYGRFKITTRFRTDSGCYATTGLKMKHL LTGQERTVWHLQYTDWPEHGCPEDLKGFLSYLEEIQSVRRHTNSTSEPRSPNPPLLVHCSAGVGRTGVVI LSEIMVACLEHNEVLDIPRVLELLRQQRMMLVQTLSQYTFVYRVLIQFLKSSRLI SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133545
ORF Size	3525 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133545.1, NP_598229.1
RefSeq Size	5543 bp
RefSeq ORF	3528 bp
Locus ID	171070
UniProt ID	Q62728
MW	133.4 kDa
Gene Summary	protein tyrosine phosphatase with similarity to the band 4.1 family of cytoskeleton-associated proteins; may play a role in cytoskeletal organization [RGD, Feb 2006]

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