Atp2b2 (NM_012508) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PMCA2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201101 representing NM_012508 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGATATGACCAACAGCGACTTTTACTCCAAAAACCAAAGAAATGAGTCGAGCCATGGGGGCGAGT TCGGGTGCAGCATGGAGGAGCTGCGCTCCCTCATGGAGCTGCGGGGCACTGAGGCTGTGGTCAAGATCAA GGAGACATATGGGGACACTGAATCCATCTGCCGGCGCCTCAAAACCTCTCCTGTTGAAGGTTTGCCAGGC ACCGCTCCAGACCTGGAAAAGAGGAAACAGATTTTCGGGCAAAACTTCATACCTCCAAAGAAACCAAAAA CCTTCCTGCAGCTGGTGTGGGAAGCACTACAGGACGTGACGCTCATCATCCTGGAGATCGCGGCCATCAT CTCCCTGGGACTGTCCTTCTACCACCCACCCGGAGAGAGCAATGAAGGATGTGCCACGGCCCAAGGTGGG GCAGAAGATGAAGGTGAAGCAGAAGCAGGCTGGATCGAGGGGGCTGCCATCCTGCTGTCAGTCATCTGTG TGGTCCTCGTCACAGCCTTCAATGATTGGAGTAAAGAGAAACAGTTCCGGGGCCTGCAGAGCCGAATTGA GCAGGAACAGAAGTTCACCGTAGTCCGGGCTGGCCAGGTGGTCCAGATCCCTGTGGCCGAGATTGTGGTT GGGGACATTGCCCAGATCAAATACGGTGACCTCCTTCCTGCTGATGGCCTCTTCATCCAGGGCAATGACC TCAAGATCGATGAAAGCTCGCTCACAGGGGAGTCTGACCAGGTGCGCAAGTCTGTGGATAAGGACCCTAT GTTGCTGTCAGGAACTCATGTGATGGAAGGCTCAGGACGGATGGTGGTGACTGCTGTGGGTGTCAACTCT CAAACTGGCATCATATTTACCCTGCTTGGGGCTGGTGGTGAAGAGGAAGAGAAGAAAGACAAAAAAGCCA AGCAGCAGGATGGGGCAGCTGCTATGGAAATGCAGCCTCTGAAGAGTGCGGAGGGCGGTGATGCAGATGA CAAGAAGAAAGCCAACATGCACAAGAAAGAGAAGTCGGTGCTTCAGGGCAAGCTCACCAAGCTGGCTGTG CAGATAGGCAAGGCGGGCCTGGTGATGTCGGCCATCACGGTGATCATCCTGGTGCTCTACTTCACCGTGG ACACCTTCGTGGTCAACAAGAAGCCGTGGCTGACGGAGTGCACACCGGTCTACGTGCAGTACTTCGTCAA GTTCTTCATCATTGGTGTGACGGTGCTGGTGGTCGCTGTGCCCGAGGGCCTCCCTCTGGCTGTCACCATC TCACTGGCCTATTCCGTGAAGAAAATGATGAAGGACAACAACCTGGTCCGTCACCTGGACGCCTGTGAGA CCATGGGTAACGCCACAGCCATCTGCTCAGACAAGACAGGCACGCTGACCACCAACCGCATGACCGTGGT GCAGGCCTATGTCGGTGATGTCCACTACAAGGAGATCCCTGATCCCAGCTCCATCAATGCCAAGACATTG GAGCTGCTGGTCAACGCCATTGCCATCAACAGCGCCTACACCACCAAGATCCTCCCCCCAGAAAAAGAGG GCGCCCTGCCCCGGCAGGTGGGCAACAAGACAGAGTGTGGCCTGCTGGGCTTCGTGCTGGACCTGAGGCA GGATTACGAGCCGGTACGCAGCCAGATGCCAGAGGAAAAGCTGTATAAGGTGTACACCTTCAACTCTGTG CGCAAGTCCATGAGCACCGTCATCAAGATGCCTGACGAGAGCTTCCGCATGTACAGCAAAGGTGCTTCGG AGATTGTGCTCAAAAAGTGCTGCAAGATTCTCAGTGGGGCAGGGGAGCCCCGTGTCTTCCGGCCCCGTGA CAGGGATGAGATGGTTAAGAAGGTGATCGAGCCCATGGCCTGTGACGGGCTCCGTACCATCTGTGTGGCC TATCGTGACTTCCCCAGCAGCCCTGAGCCTGACTGGGACAATGAGAATGACATTCTCAATGAACTCACCT GCATCTGCGTGGTGGGCATCGAAGACCCAGTGCGACCTGAGGTCCCAGAAGCCATCCGCAAGTGCCAGCG GGCCGGTATCACAGTCCGCATGGTCACCGGTGACAACATCAACACAGCCCGGGCCATCGCCATCAAGTGT GGCATTATCCACCCTGGAGAGGACTTCCTGTGCCTGGAAGGCAAAGAATTCAATCGGAGGATCCGCAACG AGAAGGGGGAGATTGAGCAGGAGCGGATTGACAAGATCTGGCCAAAGCTGAGGGTGCTGGCTCGATCCTC GCCCACGGATAAGCACACCCTGGTCAAAGGCATCATCGACAGCACACACACTGAGCAGCGGCAGGTGGTG GCTGTGACAGGGGATGGGACCAACGATGGGCCTGCTCTCAAGAAGGCAGATGTGGGCTTCGCGATGGGCA TCGCAGGCACGGATGTGGCCAAGGAGGCCTCAGACATCATCCTGACAGACGACAACTTCAGCAGCATCGT TAAGGCAGTGATGTGGGGCCGTAATGTCTATGACAGCATATCCAAATTCCTGCAGTTTCAGCTGACTGTC AATGTGGTGGCTGTGATCGTGGCCTTCACGGGTGCCTGCATTACACAGGACTCCCCTCTCAAGGCGGTGC AGATGCTCTGGGTGAACCTCATCATGGACACATTTGCTTCCCTGGCCCTGGCAACAGAGCCACCCACCGA GACTCTGCTCCTGAGGAAACCTTACGGCCGCAACAAGCCGCTCATCTCGAGAACCATGATGAAGAACATC CTGGGCCACGCCGTCTACCAGCTAACCCTCATCTTCACCCTGCTCTTCGTGGGTGAGAAGATGTTCCAGA TTGACAGCGGAAGGAACGCCCCGCTGCACTCGCCACCCTCGGAGCACTACACCATCATCTTCAACACCTT CGTCATGATGCAGCTTTTCAACGAGATCAACGCCCGTAAGATCCACGGCGAGCGTAACGTCTTCGACGGA ATCTTCCGGAACCCCATCTTCTGCACCATCGTTCTCGGCACTTTTGCCATCCAGATAGTGATTGTGCAGT TCGGCGGCAAACCCTTCAGCTGCTCCCCACTCCAGCTGGACCAGTGGATGTGGTGCATCTTCATAGGCCT GGGAGAGCTCGTCTGGGGCCAGGTCATTGCCACCATCCCTACCAGCAGGCTCAAGTTTCTGAAAGAGGCA GGGCGGCTAACACAGAAGGAGGAGATCCCCGAGGAAGAGTTAAATGAGGATGTGGAAGAGATAGACCACG CAGAGCGGGAGCTTCGCCGAGGCCAGATCCTATGGTTCCGGGGCCTGAATCGGATCCAGACACAGATCCG CGTCGTGAAGGCGTTCCGTAGCTCTCTCTATGAAGGGTTAGAAAAACCCGAGTCTCGAACCTCCATCCAT AACTTCATGGCTCATCCTGAATTCCGGATCGAAGATTCCCAGCCCCACATCCCCCTCATCGATGACACCG ACCTGGAAGAAGATGCCGCGCTCAAGCAGAACTCGAGCCCGCCGTCCTCGCTCAACAAGAACAATAGCGC CATCGACAGCGGGATCAACCTGACGACCGACACGAGCAAATCAGCTACCTCTTCAAGTCCAGGGAGCCCC ATCCACAGCCTGGAGACGTCGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201101 representing NM_012508 Red=Cloning site Green=Tags(s) MGDMTNSDFYSKNQRNESSHGGEFGCSMEELRSLMELRGTEAVVKIKETYGDTESICRRLKTSPVEGLPG TAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGESNEGCATAQGG AEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVV GDIAQIKYGDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMVVTAVGVNS QTGIIFTLLGAGGEEEEKKDKKAKQQDGAAAMEMQPLKSAEGGDADDKKKANMHKKEKSVLQGKLTKLAV QIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLTECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTI SLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINAKTL ELLVNAIAINSAYTTKILPPEKEGALPRQVGNKTECGLLGFVLDLRQDYEPVRSQMPEEKLYKVYTFNSV RKSMSTVIKMPDESFRMYSKGASEIVLKKCCKILSGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVA YRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKC GIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTHTEQRQVV AVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTV NVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNI LGHAVYQLTLIFTLLFVGEKMFQIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDG IFRNPIFCTIVLGTFAIQIVIVQFGGKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIPTSRLKFLKEA GRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESRTSIH NFMAHPEFRIEDSQPHIPLIDDTDLEEDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSP IHSLETSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012508
ORF Size	3594 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012508.5, NP_036640.1
RefSeq Size	7019 bp
RefSeq ORF	3597 bp
Locus ID	24215
UniProt ID	P11506
MW	132.6 kDa
Gene Summary	regulates intracellular calcium ion levels [RGD, Feb 2006]

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