Ehmt2 (NM_212463) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Bat8; G9a; Ng36
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201086 representing NM_212463 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGTCTGCCGAGAGGGAGGGGGCTGATGCGGGCCCGGGGGCGGGGGCGTGCGGCCCCCACGGGCG GCCGCGGCCGGGGTCGCGGGGGCGCCCACCGTGGACGAGGTAGGCCCCGAAGCCTGCTCTCGCTGCCCAG GGCCCAGGCGTCTTGGGCCCCCCAGCTGCCTGCCGGGCTGACCGGCCCTCCGGTCCCTTGTCTCCCCTCC CAGGGGGAGGCCCCCGCTGAGATGGGGGCGCTGCTGCTGGAGAAGGAGCCCCGAGGAGCCGCCGAGAGAG TTCATGGCTCTTTGGGGGACACCTCTCATAGTGAGGAGACCCTTCCCAAGGCCAACCCCGACTCCTTGGA GCCTACCGGCCCCTCCTCTCCGGCCTCTGTCACTGTCACCGTCGGCGATGAGGGGGCTGACACCCCTGTC GGGGCCACACCGCTCATCGGGGAGGAACCCGAGAACCTGGAGGGAGATGGGGGTCGAATCCTGCTGGGCC ATGCCACAAAGTCATTCCCCTCTTCCCCCAGCAAGGGGGGTGCCTGTCCCAGTCGGGCCAAAATGTCAAT GACAGGGGCAGGAAAGTCGCCCCCCTCGGTCCAGAGTTTGGCCATGAGGCTGTTGAGCATGCCTGGGGCC CAGGGAGCTGCAACTGCTGGGCCTGAGCCCCCTCCGGCAACAACTGCCGGCCAGGAGGGGCAGCCCAAAG TACACCGAGCTCGGAAAACCATGTCCAAGCCTAGCAACGGACAGCCTCCAGTCCCTGAGAAGCGGCCCCC TGAAGTCCAGCATTTCCGCATGAGTGACGACATGCATCTGGGAAAGGTGACTTCAGATGTGGCCAAACGG AGGAAGTTGACCTCGGGCAGCCTGTCAGAGGACTTGGGCTCTGCTGGGGGCTCAGGAGAAGTGATCCTGG AGAAGGGAGAGCCCAGGCCTCTGGAGGAGTGGGAGACGGTGGTGGGCGATGACTTCAGCCTGTACTATGA CGCGTACTCTGTGGATGAGCGGGTGGACTCTGACAGCAAGTCTGAAGTCGAAGCTCTAGCTGAACAGTTG AGTGAAGAGGAGGAGGAGGAAGAGGAGGAAGAAGAAGAGGAAGAGGAGGAAGAGGAGGAGGAGGAAGAGG AGGAGGAGGATGAGGAGTCGGGCAACCAGTCAGACAGGAGTGGCTCTAGCGGCCGACGCAAGGCCAAGAA GAAATGGCGGAAAGACAGCCCATGGGTGAAGCCATCCAGGAAACGGCGGAAGCGAGAGCCTCCGAGGGCC AAGGAGCCCAGAGGAGTGAATGGTGTGGGTTCCTCAGGGCCCAGTGAGTACATGGAGGTTCCTCTGGGGT CCCTGGAGCTGCCCAGCGAGGGGACCCTCTCCCCCAACCACGCTGGGGTCTCCAATGACACGTCTTCGCT GGAGACGGAGCGTGGGTTTGAGGAGCTGCCCCTCTGCAGCTGCCGCATGGAGGCGCCCAAGATTGACCGC ATCAGCGAGAGAGCAGGACACAAGTGTATGGCCACCGAAAGTGTGGACGGAGAGCTGCTGGGTTGCAATG CCGCCATCTTGAAGCGGGAGACCATGCGGCCGTCTAGCCGTGTGGCGCTGATGGTACTGTGTGAGGCCCA TCGAGCCCGCATGGTCAAGCACCATTGCTGCCCAGGCTGTGGCTACTTCTGCACGGCGGGCACCTTCCTG GAGTGTCACCCCGACTTCCGTGTAGCTCACCGCTTCCATAAGGCCTGCGTGTCCCAGCTCAATGGGATGG TTTTCTGTCCCCACTGTGGGGAGGACGCCTCAGAGGCCCAGGAGGTGACCATCCCTCGGGGCGATGGGGG AACACCCCCGGTTGGCACTGTGGCGCCTGCCCCGCCACCCCTGGCACACGACGCCCCAGGGCGAGCGGAT ACCTCCCAGCCCAGTGCCCGAATGCGAGGACACGGAGAGCCAAGGCGCCCACCCTGTGATCCCCTGGCCG ACACCATTGACAGCTCGGGGCCTTCTCTGACTCTGCCTAATGGCGGCTGCCTCTCAGCTGTGGGTCTGCC CCCAGGGCCAGGCCGGGAAGCCCTGGAAAAAGCCTTGGTCATCCAGGAGTCCGAGAGGAGGAAGAAGCTG CGGTTCCACCCGCGGCAGCTCTACCTGTCGGTGAAGCAGGGGGAGCTGCAGAAGGTGATCCTCATGCTGC TCGACAACCTGGACCCCAACTTCCAGAGCGACCAGCAGAGCAAGCGCACGCCCCTGCACGCAGCCGCCCA GAAGGGGTCCGTGGAGATCTGTCACGTGCTGCTACAGGCAGGAGCCAACATCAACGCCGTGGATAAGCAG CAGCGCACCCCGCTGATGGAGGCCGTGGTGAACAACCACCTGGAGGTGGCTCGCTACATGGTGCAGCTGG GCGGCTGTGTGTACAGCAAGGAAGAGGATGGCTCTACCTGTCTCCATCATGCGGCCAAAATTGGGAACTT GGAAATGGTCAGCCTGCTGCTGAGCACAGGGCAGGTGGACGTCAATGCCCAGGACAGTGGGGGCTGGACA CCCATCATCTGGGCAGCCGAGCACAAGCACATCGACGTGATCCGTATGCTGCTGACCCGGGGCGCCGACG TCACCCTTACCGACAACGAGGAAAACATCTGCCTGCACTGGGCTTCCTTCACGGGTAGTGCCGCTATTGC CGAGGTCCTCCTGAATGCCCAGTGCGACCTCCATGCTGTCAACTACCACGGGGACACGCCCCTGCACATA GCCGCCAGGGAGAGCTACCATGACTGCGTTCTGTTGTTCCTGTCCCGTGGAGCCAACCCTGAGCTTCGGA ACAAAGAAGGGGACACGGCGTGGGATCTGACCCCAGAGCGCTCCGATGTGTGGTTTGCACTGCAGCTCAA TCGCAAGCTTAGGCTCGGGGTAGGGAACCGGGCTGTCCGCACCGAGAAGATCATCTGCCGGGATGTAGCC CGAGGCTATGAGAATGTGCCCATTCCCTGTGTCAATGGCGTGGATGGGGAGCCATGCCCAGAGGACTATA AGTACATCTCCGAGAACTGTGAAACGTCCACCATGAACATCGACCGAAACATCACTCACCTGCAGCACTG CACGTGTGTTGACGACTGCTCCAGCTCCAACTGCCTGTGTGGCCAGCTAAGCATCCGATGCTGGTATGAT AAGGACGGGCGGCTGCTCCAGGAGTTTAACAAGATCGAGCCCCCCCTGATCTTTGAGTGTAACCAGGCAT GCTCCTGCTGGAGAAGCTGCAAGAACCGCGTGGTGCAGAGCGGCATCAAGGTGCGGCTGCAGCTCTACCG GACGGCCAAGATGGGCTGGGGGGTCCGTGCCTTGCAGACCATTCCCCAGGGCACGTTCATCTGCGAGTAC GTCGGGGAGCTGATCTCCGACGCCGAGGCAGACGTGAGAGAGGATGATTCTTACCTCTTCGACTTAGACA ACAAGGATGGTGAGGTCTACTGCATTGATGCCCGTTACTATGGCAACATCAGCCGCTTCATTAACCACCT GTGCGACCCCAACATCATCCCTGTTCGGGTCTTCATGCTGCATCAAGACCTACGGTTCCCTCGCATCGCC TTCTTCAGCTCCAGGGACATCCGGACCGGGGAGGAGCTGGGGTTTGACTACGGCGACCGGTTCTGGGATA TCAAGAGCAAGTATTTCACCTGCCAGTGTGGCTCTGAGAAGTGCAAGCATTCAGCTGAGGCCATCGCCCT GGAGCAGAGCCGCCTGGCCCGTCTGGACCCCCACCCGGAGCTGCTCCCTGACCTCAGCTCCCTGCCCCCC ATCAACACC CTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG ATAAGGTTTAA Protein Sequence (showhide) >RR201086 representing NM_212463 Red=Cloning site Green=Tags(s) MRGLPRGRGLMRARGRGRAAPTGGRGRGRGGAHRGRGRPRSLLSLPRAQASWAPQLPAGLTGPPVPCLPS QGEAPAEMGALLLEKEPRGAAERVHGSLGDTSHSEETLPKANPDSLEPTGPSSPASVTVTVGDEGADTPV GATPLIGEEPENLEGDGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGA QGAATAGPEPPPATTAGQEGQPKVHRARKTMSKPSNGQPPVPEKRPPEVQHFRMSDDMHLGKVTSDVAKR RKLTSGSLSEDLGSAGGSGEVILEKGEPRPLEEWETVVGDDFSLYYDAYSVDERVDSDSKSEVEALAEQL SEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRA KEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDR ISERAGHKCMATESVDGELLGCNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCCPGCGYFCTAGTFL ECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGGTPPVGTVAPAPPPLAHDAPGRAD TSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKL RFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQ QRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWT PIIWAAEHKHIDVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNAQCDLHAVNYHGDTPLHI AARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAVRTEKIICRDVA RGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYD KDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEY VGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIA FFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPDLSSLPP INT LEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-XhoI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_212463
ORF Size	3789 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_212463.1, NP_997628.1
RefSeq Size	4023 bp
RefSeq ORF	3792 bp
Locus ID	361798
MW	138.1 kDa
Gene Summary	mouse homolog may act as a histone methyltransferase and may be involved in genetic imprinting [RGD, Feb 2006]

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