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Rhbdf1 (NM_001030034) Rat Tagged ORF Clone
CAT＃: RR201047
- TrueORF®
Rhbdf1 (Myc-DDK-tagged ORF) - Rat rhomboid 5 homolog 1 (Drosophila) (Rhbdf1), (10 ug)
"NM_001030034" in other vectors (3)
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CNY 10,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC124815 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201047 representing NM_001030034
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAAGCCCGGAGAGACAGTACTAGCAGTCTGCAACGCAAGAAACCCCCGTGGCTCAAGTTGGACA TCCCAGCTGTGGTGCCCCCAGCAGCAGAAGAGCCCAGCTTCCTGCAGCCCCTGCGGCGACAGGCTTTCCT GCGGAGTGTGAGTATGCCGGCTGAGACAGCCCGTGTCCCGTCACCCCACCATGAGCCTCGGCGGCTGGCG CTGCAGCGTCAGACATCCATCACGCAGACCATCCGCAGGGGGACAGCTGACTGGTTTGGAGTGAGCAAGG ACAGTGACAGCACCCAGAAGTGGCAGCGTAAGAGCATCCGTCACTGCAGCCAGCGATATGGGAAGCTAAA GCCACAGGTCATCCGGGAGCTGGACCTGCCCAGCCAGGACAATGTGTCGCTGACCAGCACTGAGACACCG CCTCCACTCTATGTGGGGCCATGCCAGCTGGGAATGCAGAAGATCATAGACCCTCTGGCCCGAGGCCGGG CCTTCCGCATGGCCGATGACACCGCCGATGGCCTGAGTGCCCCCCACACTCCCGTCACACCAGGTGCTGC CTCCCTCTGTTCCTTCTCCAGCTCCCGCTCAGGTTTCAACCGGCTCCCTCGGCGTCGCAAACGTGAATCC GTGGCCAAGATGAGCTTCCGGGCCGCTGCAGCGCTGGTGAAGGGTCGGTCTATTAGGGATGGCACTTTAC GAAGGGGACAGCGTCGGAGCTTTACTCCTGCCAGCTTCCTGGAGGAAGACATGGTGGACTTCCCTGATGA GCTGGATACATCTTTCTTTGCCCGGGAAGGTGTCCTCCATGAGGAGCTGTCCACGTACCCAGATGAGGTG TTTGAGTCCCCATCAGAAGCAGCGCTCAAGGACTGGGAGAAAGCCCCGGATCAGGCAGACCTTACGGGTG GGGCCCTGGACCGCAGCGAGCTCGAGCGAAGCCACTTGATGTTACCCCTGGAACGAGGCTGGAGGAAGCA GAAGGAAGGCGGCACGCTAGCCCCGCAGCCCAAGGTGCGGCTTCGCCAGGAGGTGGTCAGTGCGGCTGGC CCCAGGAGGGGTCAGCGTATCGCTGTGCCAGTGCGCAAGCTCTTCGCCCGGGAAAAACGGCCGTATGGTC TGGGGATGGTGGGACGGCTCACCAACCGCACGTACCGGAAACGGATTGACAGCTACGTCAAGCGGCAGAT AGAAGACATGGATGATCACAGGCCCTTCTTCACTTACTGGCTCACCTTCGTGCACTCCCTGGTCACCATT CTGGCTGTGTGTATCTACGGCATCGCGCCTGTGGGCTTCTCGCAGCACGAGACGGTGGACTCGGTACTTC GGAAGCGGGGTGTCTATGAGAATGTCAAATACGTCCAGCAGGAGAACTTCTGGATTGGCCCCAGCTCGGA GGCCCTCATTCACCTGGGTGCCAAGTTCTCCCCCTGCATGCGCCAGGACCCACAGGTGCACAACTTTATC CTTGCTGCCCGGGAGCGTGAAAAGCACTCAGCCTGCTGTGTGCGCAATGACCGGTCTGGCTGTGTGCAGA CGTCGAAGGAGGAGTGCTCGTCTACGTTGGCAGTGTGGGTGAAGTGGCCGGTTCATCCTAGTGCTCCCGA CCTGGCAGGCAACAAGAGGCAGTTTGGTTCTGTCTGCCACCAGGACCCCAGGGTGTGTGATGAGCCTTCC TCTGAGGATCCCCACGAGTGGCCAGAAGATATCACCAAGTGGCCGATCTGCACCAAAAACAGCGCGGGGA ACCACACTAACCACCCTCACATGGACTGTGTCATAACGGGCAGGCCCTGCTGCATCGGCACCAAGGGCAG GTGTGAGATCACCTCCCGGGAGTACTGTGACTTCATGAAGGGCTACTTCCATGAGGAGGCCACACTCTGC TCTCAGGTGCACTGCATGGACGATGTCTGTGGCCTCCTGCCTTTCCTCAATCCCGAGGTGCCTGACCAGT TCTACCGCCTGTGGCTGTCCCTCTTCCTGCACGCTGGGATCCTGCACTGTTTGGTGTCCGTCTGCTTCCA GATGACGGTCCTGCGGGACCTGGAGAAGCTGGCAGGCTGGCACCGCATAGCCATCATCTACCTGCTGAGC GGTGTCACGGGCAATCTAGCCAGCGCCATCTTTCTGCCGTACCGGGCAGAGGTAGGTCCGGCCGGCTCCC AGTTTGGTATCCTGGCCTGTCTCTTTGTGGAGCTATTCCAGAGCTGGCAGATCCTGGCCCGACCCTGGCG TGCCTTCTTCAAGCTCCTGGCCGTGGTGCTCTTCCTCTTTGCCTTTGGGCTGCTGCCCTGGATCGACAAC TTCGCCCACATCTCAGGTTTCGTCAGCGGCCTCTTCCTTTCCTTTGCCTTCCTGCCTTACATCAGCTTTG GCAAGTTCGACCTGTACCGCAAGCGTTGCCAGATCATCATCTTCCAGGCCGTCTTCCTGGGCCTGCTGGC CGGCCTGGTGGTCCTCTTCTATTTCTACCCAGTGCGCTGTGAATGGTGTGAGTTCCTCACCTGCATCCCC TTCACAGACAAGTTCTGTGAGAAGTACGAGCTGGATGCTCAGCTCCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201047 representing NM_001030034
Red=Cloning site Green=Tags(s) MSEARRDSTSSLQRKKPPWLKLDIPAVVPPAAEEPSFLQPLRRQAFLRSVSMPAETARVPSPHHEPRRLA LQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETP PPLYVGPCQLGMQKIIDPLARGRAFRMADDTADGLSAPHTPVTPGAASLCSFSSSRSGFNRLPRRRKRES VAKMSFRAAAALVKGRSIRDGTLRRGQRRSFTPASFLEEDMVDFPDELDTSFFAREGVLHEELSTYPDEV FESPSEAALKDWEKAPDQADLTGGALDRSELERSHLMLPLERGWRKQKEGGTLAPQPKVRLRQEVVSAAG PRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTI LAVCIYGIAPVGFSQHETVDSVLRKRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHNFI LAAREREKHSACCVRNDRSGCVQTSKEECSSTLAVWVKWPVHPSAPDLAGNKRQFGSVCHQDPRVCDEPS SEDPHEWPEDITKWPICTKNSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLC SQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLS GVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFAFGLLPWIDN FAHISGFVSGLFLSFAFLPYISFGKFDLYRKRCQIIIFQAVFLGLLAGLVVLFYFYPVRCEWCEFLTCIP FTDKFCEKYELDAQLH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001030034 |
| ORF Size | 2568 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001030034.1, NP_001025205.1 |
| RefSeq Size | 2943 bp |
| RefSeq ORF | 2571 bp |
| Locus ID | 303008 |
| UniProt ID | Q499S9 |
| MW | 97.3 kDa |
| Gene Summary | Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201047 | Rhbdf1 (untagged ORF) - Rat rhomboid 5 homolog 1 (Drosophila) (Rhbdf1), (10 ug) |
CNY 10,930.00 |
|
| RR201047L3 | Lenti ORF clone of Rhbdf1 (Myc-DDK-tagged ORF) - Rat rhomboid 5 homolog 1 (Drosophila) (Rhbdf1), (10 ug) |
CNY 12,730.00 |
|
| RR201047L4 | Lenti ORF clone of Rhbdf1 (mGFP-tagged ORF) - Rat rhomboid 5 homolog 1 (Drosophila) (Rhbdf1), (10 ug) |
CNY 13,970.00 |
