Pklr (NM_012624) Rat Untagged Clone
CAT#: RN203014
Pklr (untagged ORF) - Rat pyruvate kinase, liver and RBC (Pklr), nuclear gene encoding mitochondrial protein, (10 ug)
CNY 6,940.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | PK1; PKL; Pklg |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN203014 representing NM_012624
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGGCCAGCGGGATACCTTCGACGTGCGAGTGTGGCTCAACTGACCCAGGAGCTGGGCACTGCCT TCTTCCAGCAGCAGCAACTGCCCGCAGCTATGGCGGACACCTTCCTGGAACACCTCTGCCTTCTGGATAT CGACTCACAGCCTGTGGCTGCTCGTAGCACCAGCATCATTGCCACCATTGGGCCAGCATCCCGCTCTGTG GACCGCCTCAAGGAGATGATCAAAGCAGGGATGAACATTGCACGACTCAACTTCTCCCATGGCTCCCATG AGTACCATGCAGAATCCATCGCCAACATCCGGGAGGCAACTGAGAGTTTTGCAACCTCCCCACTCAGCTA CAGACCTGTGGCCATCGCCCTGGACACCAAGGGACCTGAGATACGAACCGGAGTCTTGCAGGGGGGTCCG GAGTCGGAGGTGGAAATTGTGAAGGGCTCACAGGTGCTGGTGACGGTGGACCCGAAGTTCCAGACAAGGG GCGATGCAAAGACAGTGTGGGTGGACTACCACAATATCACCCGGGTCGTTGCAGTGGGGGGCCGCATCTA CATTGACGACGGGCTCATCTCCTTAGTGGTACAGAAAATCGGCCCAGAGGGACTGGTGACAGAAGTGGAG CACGGTGGTATCTTGGGCAGCAGGAAGGGTGTGAACTTGCCAAACACTGAGGTGGACCTGCCCGGGCTGT CTGAGCAAGACCTTTTGGATCTGCGCTTCGGGGTGCAGCATAATGTGGACATCATCTTTGCCTCCTTTGT GCGGAAAGCCAGTGACGTGTTAGCAGTCCGGGATGCCCTGGGGCCAGAAGGACAGAACATCAAAATTATC AGCAAAATCGAGAACCATGAAGGCGTGAAGAAGTTTGATGAAATTCTAGAAGTGAGCGATGGCATCATGG TGGCACGGGGTGACCTGGGCATTGAGATCCCTGCGGAGAAGGTTTTCTTGGCTCAGAAGATGATGATTGG ACGCTGCAACCTGGCCGGCAAGCCTGTCGTTTGTGCCACACAGATGCTGGAGAGCATGATCACTAAGGCT CGACCAACTCGGGCGGAGACAAGCGATGTGGCCAATGCCGTGCTGGATGGGGCTGACTGTATCATGCTGT CCGGAGAGACCGCCAAGGGCAGTTTTCCTGTGGAAGCTGTAATGATGCAACATGCGATTGCGCGGGAGGC AGAGGCCGCTGTGTACCACCGCCAGTTGTTTGAGGAGCTACGCCGGGCAGCGCCGCTGAGCCGTGACCCA ACTGAGGTCACTGCGATTGGAGCCGTGGAGGCTTCCTTCAAGTGCTGTGCAGCAGCCATCATCGTGCTGA CGAAGACTGGCCGTTCAGCCCAGCTTCTATCTCAATACCGACCTCGGGCGGCTGTCATTGCTGTGACTCG ATCTGCCCAGGCTGCCCGACAGGTCCACCTGTCCCGAGGAGTCTTCCCCTTGCTCTACCGTGAGCCTCCA GAGGCCATCTGGGCAGATGATGTGGATCGAAGGGTCCAATTTGGCATTGAAAGTGGAAAGCTCCGTGGTT TCCTCCGTGTGGGTGATCTGGTGATTGTGGTGACAGGTTGGCGGCCTGGCTCTGGCTATACCAACATCAT GCGGGTGCTGAGCGTATCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_012624 |
Insert Size | 1632 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_012624.3, NP_036756.3 |
RefSeq Size | 2922 bp |
RefSeq ORF | 1632 bp |
Locus ID | 24651 |
UniProt ID | P12928 |
Gene Summary | L-isozyme of pyruvate kinase; catalyzes the conversion of ATP and pyruvate to ADP and phosphoenolpyruvate in glycolysis [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR203014 | Pklr (Myc-DDK-tagged ORF) - Rat pyruvate kinase, liver and RBC (Pklr), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,940.00 |
|
RR203014L3 | Lenti ORF clone of Pklr (Myc-DDK-tagged ORF) - Rat pyruvate kinase, liver and RBC (Pklr), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 8,930.00 |
|
RR203014L4 | Lenti ORF clone of Pklr (mGFP-tagged ORF) - Rat pyruvate kinase, liver and RBC (Pklr), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 9,790.00 |