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Gemin 5 (GEMIN5) (NM_001252156) Human Tagged ORF Clone
CAT＃: RG233213
- TrueORF®
GEMIN5 (tGFP-tagged) - Homo sapiens gem (nuclear organelle) associated protein 5 (GEMIN5), transcript variant 2
ORF Plasmid: DDK
"NM_001252156" in other vectors (2)
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CNY 16,530.00
货期*
7周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GEMIN-5; NEDCAM |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233213 representing NM_001252156
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAGGAGCCGCGGACGCTGCCGCCCTCCCCCAACTGGTACTGCGCCCGCTGCAGCGATGCCGTGC CCGGGGGCCTCTTTGGCTTCGCCGCGCGGACCTCCGTCTTCCTTGTCCGCGTGGGCCCGGGCGCAGGCGA GAGTCCAGGGACACCCCCGTTTCGAGTCATAGGAGAGTTGGTGGGACACACCGAAAGGGTCTCTGGCTTC ACATTTTCTCATCACCCTGGTCAGTACAACCTCTGTGCCACCAGCTCCGACGATGGGACTGTGAAAATAT GGGATGTAGAGACAAAAACAGTTGTGACAGAACATGCACTCCATCAGCATACGATATCAACATTACATTG GTCTCCTCGAGTAAAGGACTTAATAGTATCTGGGGATGAAAAAGGAGTAGTTTTCTGTTACTGGTTTAAC AGAAATGACAGCCAGCACCTCTTTATAGAACCCAGGACAATTTTCTGTCTTACTTGTTCACCTCATCATG AAGATTTAGTAGCCATTGGCTACAAGGATGGCATAGTGGTGATAATTGACATCAGTAAGAAAGGAGAAGT TATTCATAGGCTTCGAGGCCATGATGATGAAATCCACTCCATAGCCTGGTGTCCCCTGCCTGGTGAAGAT TGTTTATCTATAAACCAAGAGGAAACTTCAGAAGCTGAAATTACCAACGGGAATGCTGTAGCACAAGCTC CAGTAACAAAAGGTTGCTACTTAGCCACTGGAAGCAAAGATCAAACCATTCGAATCTGGAGCTGTTCTAG AGGCCGAGGGGTGATGATTTTGAAATTGCCCTTTCTGAAGAGAAGAGGAGGGGGTATAGACCCAACTGTT AAAGAGCGCCTTTGGTTGACACTCCATTGGCCCAGCAATCAACCAACACAGCTGGTATCTAGCTGTTTTG GAGGTGAACTGTTGCAATGGGATCTCACTCAATCTTGGAGACGGAAATACACCCTCTTCAGTGCCTCATC AGAAGGGCAAAATCATTCAAGAATTGTGTTTAATTTATGTCCTTTACAAACAGAGGATGACAAACAGCTA TTACTTTCTACATCAATGGATAGAGATGTAAAATGTTGGGACATAGCCACCTTGGAGTGCAGCTGGACCC TTCCTTCCCTTGGTGGGTTTGCATACAGCCTGGCTTTCTCTTCTGTGGACATAGGCTCTTTGGCCATAGG TGTTGGGGATGGCATGATCCGTGTATGGAATACACTCTCCATAAAGAACAACTATGATGTGAAAAATTTT TGGCAAGGCGTGAAGTCCAAGGTTACAGCGCTGTGCTGGCACCCAACCAAGGAAGGTTGCTTAGCTTTTG GAACTGATGATGGAAAAGTGGGATTGTATGACACCTACTCCAACAAGCCTCCACAGATTTCTAGCACATA TCATAAGAAGACTGTATATACTTTAGCCTGGGGGCCACCAGTACCCCCCATGTCACTTGGAGGAGAAGGA GACAGACCTTCCCTTGCTTTATACAGCTGTGGAGGAGAAGGGATTGTCTTACAGCATAATCCCTGGAAGC TTAGTGGAGAAGCCTTTGACATCAACAAACTCATCAGGGACACCAATTCAATCAAATACAAATTGCCTGT ACACACAGAGATAAGTTGGAAAGCAGATGGCAAAATCATGGCTCTTGGCAATGAAGATGGATCAATAGAA ATATTTCAGATTCCCAACCTGAAACTGATCTGTACTATCCAACAGCATCACAAGCTTGTGAATACCATTA GCTGGCATCATGAGCATGGCAGCCAGCCAGAATTGAGCTATCTGATGGCCTCTGGCTCCAACAATGCAGT CATTTACGTGCACAACCTGAAGACTGTCATAGAGAGCAGCCCTGAGTCTCCAGTGACCATTACAGAGCCC TACCGGACCCTCTCAGGGCATACGGCCAAGATTACCAGTGTGGCGTGGAGCCCACATCATGATGGAAGGC TGGTATCTGCTTCCTATGATGGTACAGCCCAGGTGTGGGATGCTCTCCGGGAAGAGCCCCTGTGCAATTT CCGAGGACATCGAGGTCGACTGCTTTGTGTGGCATGGTCTCCTTTGGATCCAGACTGCATCTATTCAGGG GCAGATGACTTTTGTGTGCACAAGTGGCTCACTTCCATGCAAGATCATTCCCGGCCTCCTCAAGGCAAAA AAAGTATTGAATTGGAGAAAAAACGGCTCTCTCAACCTAAGGCAAAGCCCAAAAAGAAGAAAAAGCCCAC CTTGAGAACTCCTGTAAAGCTGGAATCGATTGATGGAAATGAAGAAGAAAGCATGAAGGAGAACTCAGGA CCTGTTGAGAATGGTGTGTCAGACCAAGAAGGGGAGGAGCAAGCACGGGAGCCGGAATTACCCTGTGGCC TTGCTCCAGCGGTTTCTAGAGAACCAGTTATCTGCACTCCAGTTTCCTCAGGCTTTGAAAAGTCAAAAGT CACCATTAATAACAAAGTCATTTTACTGAAAAAGGAGCCACCAAAAGAGAAGCCAGAAACCTTAATCAAG AAGAGAAAAGCTCGTTCCTTGCTTCCCCTGAGTACAAGCCTGGACCACAGATCCAAAGAGGAGCTTCATC AGGACTGTTTGGTACTAGCAACTGCAAAGCACTCCAGAGAGCTGAATGAAGATGTGTCTGCTGATGTTGA GGAAAGATTTCATCTGGGGCTTTTCACAGACAGGGCTACCCTGTATAGAATGATTGATATTGAAGGAAAA GGTCACTTAGAAAATGGCCACCCTGAGTTATTTCACCAGCTTATGCTTTGGAAAGGAGATCTCAAAGGTG TTCTCCAGACTGCAGCAGAAAGAGGGGAGCTGACAGACAACCTTGTGGCTATGGCACCAGCAGCTGGCTA CCATGTGTGGCTATGGGCTGTGGAAGCTTTTGCCAAACAGCTGTGTTTTCAGGATCAGTATGTCAAGGCT GCTTCTCACCTACTTTCCATCCACAAAGTGTATGAAGCGGTGGAGCTGCTCAAGTCAAACCATTTTTACA GGGAAGCTATTGCGATTGCCAAGGCCCGGCTGCGCCCGGAGGACCCAGTCCTGAAGGACTTGTACCTCAG CTGGGGAACCGTCCTAGAAAGAGATGGCCACTATGCTGTAGCTGCCAAATGCTATTTAGGGGCCACTTGT GCTTATGATGCAGCCAAAGTTTTGGCCAAAAAGGGGGATGCGGCATCACTTAGAACGGCTGCAGAGTTGG CTGCCATCGTAGGAGAGGATGAGTTGTCTGCTTCCCTGGCTCTCAGATGTGCCCAAGAGCTGCTTCTGGC CAACAACTGGGTGGGAGCCCAGGAAGCCCTGCAGCTGCATGAAAGTCTACAGGGTCAGAGATTGGTGTTT TGCCTTCTGGAGCTACTGTCCAGGCATCTGGAGGAAAAGCAGCTTTCAGAGGGCAAAAGCTCCTCCTCTT ACCACACTTGGAACACGGGCACCGAAGGGCCTTTCGTGGAGAGGGTGACTGCAGTGTGGAAGAGCATCTT CAGCCTTGACACCCCTGAGCAGTATCAGGAAGCCTTTCAGAAGCTGCAGAACATCAAGTACCCATCTGCT ACAAATAACACACCTGCCAAACAGCTCCTGCTTCACATTTGCCATGACTTGACCCTGGCAGTGCTGAGCC AACAGATGGCCTCCTGGGACGAGGCTGTGCAGGCGCTCCTTCGGGCGGTGGTCCGGAGCTATGACTCAGG GAGCTTCACCATCATGCAGGAAGTGTACTCAGCCTTTCTCCCTGACGGCTGTGACCACCTAAGAGACAAG TTGGGGGACCATCAATCCCCTGCCACACCAGCTTTCAAAAGTTTGGAGGCCTTTTTTCTTTATGGGCGTC TGTATGAATTCTGGTGGTCTCTCTCCAGACCTTGCCCAAATTCCAGTGTCTGGGTAAGGGCTGGTCACAG AACACTCTCTGTTGAGCCAAGCCAGCAGTTAGACACTGCCAGCACTGAAGAAACGGACCCTGAAACTTCT CAGCCAGAGCCAAACAGGCCTTCAGAACTAGACTTGAGACTCACAGAAGAAGGTGAGCGAATGCTGAGTA CTTTTAAGGAGCTCTTTTCAGAAAAGCATGCCAGTCTCCAAAACTCACAGAGAACTGTTGCTGAAGTCCA AGAGACCTTGGCAGAAATGATCCGACAACACCAAAAGAGTCAACTCTGTAAATCCACAGCAAATGGTCCT GATAAGAATGAACCGGAAGTAGAAGCAGAGCAGCCCCTCTGCAGTTCTCAGAGCCAGTGTAAAGAAGAAA AAAATGAGCCACTTTCTCTGCCTGAGTTAACCAAAAGGCTTACCGAGGCAAATCAGAGAATGGCGAAATT TCCTGAGAGCATTAAGGCCTGGCCCTTCCCAGATGTGCTGGAGTGCTGCCTCGTCCTGCTTCTCATCAGG TCCCACTTTCCTGGCTGTCTGGCCCAGGAAATGCAGCAGCAGGCCCAAGAGCTCCTTCAGAAATACGGCA ACACGAAAACTTACAGAAGACACTGCCAGACCTTCTGTATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233213 representing NM_001252156
Red=Cloning site Green=Tags(s) MGQEPRTLPPSPNWYCARCSDAVPGGLFGFAARTSVFLVRVGPGAGESPGTPPFRVIGELVGHTERVSGF TFSHHPGQYNLCATSSDDGTVKIWDVETKTVVTEHALHQHTISTLHWSPRVKDLIVSGDEKGVVFCYWFN RNDSQHLFIEPRTIFCLTCSPHHEDLVAIGYKDGIVVIIDISKKGEVIHRLRGHDDEIHSIAWCPLPGED CLSINQEETSEAEITNGNAVAQAPVTKGCYLATGSKDQTIRIWSCSRGRGVMILKLPFLKRRGGGIDPTV KERLWLTLHWPSNQPTQLVSSCFGGELLQWDLTQSWRRKYTLFSASSEGQNHSRIVFNLCPLQTEDDKQL LLSTSMDRDVKCWDIATLECSWTLPSLGGFAYSLAFSSVDIGSLAIGVGDGMIRVWNTLSIKNNYDVKNF WQGVKSKVTALCWHPTKEGCLAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYTLAWGPPVPPMSLGGEG DRPSLALYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKLPVHTEISWKADGKIMALGNEDGSIE IFQIPNLKLICTIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAVIYVHNLKTVIESSPESPVTITEP YRTLSGHTAKITSVAWSPHHDGRLVSASYDGTAQVWDALREEPLCNFRGHRGRLLCVAWSPLDPDCIYSG ADDFCVHKWLTSMQDHSRPPQGKKSIELEKKRLSQPKAKPKKKKKPTLRTPVKLESIDGNEEESMKENSG PVENGVSDQEGEEQAREPELPCGLAPAVSREPVICTPVSSGFEKSKVTINNKVILLKKEPPKEKPETLIK KRKARSLLPLSTSLDHRSKEELHQDCLVLATAKHSRELNEDVSADVEERFHLGLFTDRATLYRMIDIEGK GHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLVAMAPAAGYHVWLWAVEAFAKQLCFQDQYVKA ASHLLSIHKVYEAVELLKSNHFYREAIAIAKARLRPEDPVLKDLYLSWGTVLERDGHYAVAAKCYLGATC AYDAAKVLAKKGDAASLRTAAELAAIVGEDELSASLALRCAQELLLANNWVGAQEALQLHESLQGQRLVF CLLELLSRHLEEKQLSEGKSSSSYHTWNTGTEGPFVERVTAVWKSIFSLDTPEQYQEAFQKLQNIKYPSA TNNTPAKQLLLHICHDLTLAVLSQQMASWDEAVQALLRAVVRSYDSGSFTIMQEVYSAFLPDGCDHLRDK LGDHQSPATPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVRAGHRTLSVEPSQQLDTASTEETDPETS QPEPNRPSELDLRLTEEGERMLSTFKELFSEKHASLQNSQRTVAEVQETLAEMIRQHQKSQLCKSTANGP DKNEPEVEAEQPLCSSQSQCKEEKNEPLSLPELTKRLTEANQRMAKFPESIKAWPFPDVLECCLVLLLIR SHFPGCLAQEMQQQAQELLQKYGNTKTYRRHCQTFCM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001252156 |
| ORF Size | 4521 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001252156.1, NP_001239085.1 |
| RefSeq Size | 5401 bp |
| RefSeq ORF | 4524 bp |
| Locus ID | 25929 |
| UniProt ID | Q8TEQ6 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
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