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ELMO1 (NM_001206480) Human Tagged ORF Clone
CAT＃: RG233105
- TrueORF®
ELMO1 (tGFP-tagged) - Homo sapiens engulfment and cell motility 1 (ELMO1), transcript variant 4
ORF Plasmid: DDK
"NM_001206480" in other vectors (2)
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CNY 4,370.00
货期*
3周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CED-12; CED12; ELMO-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233105 representing NM_001206480
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCCACCCGCGGACATCGTCAAGGTGGCCATAGAATGGCCGGGCGCCTACCCCAAACTCATGGAAA TTGATCAGAAAAAACCACTGTCTGCAATAATAAAGGAAGTCTGTGATGGGTGGTCTCTTGCCAACCATGA ATATTTTGCACTCCAGCATGCCGATAGTTCAAACTTCTATATCACAGAAAAGAACCGCAATGAGATAAAA AATGGCACTATCCTTCGATTAACCACATCTCCAGCTCAGAACGCCCAGCAGCTCCATGAACGAATCCAGT CCTCGAGTATGGATGCCAAGCTGGAAGCCCTGAAGGACTTGGCCAGCCTCTCCCGGGATGTCACGTTTGC CCAGGAGTTTATAAACCTGGACGGTATCTCTCTCCTCACGCAGATGGTGGAGAGCGGCACTGAGCGATAC CAGAAATTGCAGAAGATCATGAAGCCTTGCTTTGGAGACATGCTGTCCTTCACCCTGACGGCCTTCGTTG AGCTGATGGACCATGGCATAGTGTCCTGGGATACATTTTCGGTGGCGTTCATTAAGAAGATAGCAAGTTT TGTGAACAAGTCAGCCATAGACATCTCGATCCTGCAGCGGTCCTTGGCCATTTTGGAGTCGATGGTGCTC AATAGCCATGACCTCTACCAGAAAGTGGCGCAGGAGATCACCATCGGCCAGCTCATTCCACACCTGCAAG GGTCAGATCAAGAAATCCAAACCTATACTATTGCAGTGATTAATGCGCTTTTCCTGAAGGCTCCTGATGA GAGGAGGCAGGAGATGGCGAATATTTTGGCTCAGAAGCAACTGCGTTCCATCATTTTAACACATGTCATC CGAGCCCAGCGGGCCATCAACAATGAGATGGCGCACCAGCTGTATGTTCTACAAGTGCTCACCTTTAACC TCCTGGAAGACAGGATGATGACCAAAATGGACCCCCAGGACCAGGCTCAGAGGGACATCATATTTGAACT TCGAAGAATTGCTTTTGATGCTGAGTCTGAACCTAACAACAGCAGTGGCAGCATGGAGAAACGCAAGTCC ATGTACACGCGAGATTATAAGAAGCTTGGGTTCATTAATCATGTCAACCCTGCCATGGACTTCACGCAGA CTCCACCTGGGATGTTGGCTCTGGACAACATGCTGTACTTTGCCAAGCACCACCAAGATGCCTACATCCG GATTGTGCTTGAGAACAGTAGTCGAGAAGACAAGCATGAATGTCCCTTTGGCCGCAGTAGTATAGAGCTG ACCAAGATGCTATGTGAGATCTTGAAAGTGGGCGAGTTGCCTAGTGAGACCTGCAACGACTTCCACCCGA TGTTCTTCACCCACGACAGATCCTTTGAGGAGTTTTTCTGCATCTGTATCCAGCTCCTGAACAAGACATG GAAGGAAATGAGGGCAACTTCTGAAGACTTCAACAAGGTAATGCAGGTGGTGAAGGAGCAGGTTATGAGA GCACTTACAACCAAGCCTAGCTCCCTGGACCAGTTCAAGAGCAAACTGCAGAACCTGAGCTACACTGAGA TCCTGAAAATCCGCCAGTCCGAGAGGATGAACCAGGAAGATTTCCAGTCCCGCCCGATTTTGGAACTAAA GGAGAAGATTCAGCCAGAAATCTTAGAGCTGATCAAACAGCAACGCCTGAACCGCCTTGTGGAAGGGACC TGCTTTAGGAAACTCAATGCCCGGCGGAGGCAAGACAAGTTTTGGTATTGTCGGCTTTCGCCAAATCACA AAGTCCTGCATTACGGAGACTTAGAAGAGAGTCCTCAGGGAGAAGTGCCCCACGATTCCTTGCAGGACAA ACTGCCGGTGGCAGATATCAAAGCCGTGGTGACGGGAAAGGACTGCCCTCATATGAAAGAGAAAGGTGCC CTTAAACAAAACAAGGAGGTGCTTGAACTCGCTTTCTCCATCTTGTATGACTCAAACTGCCAACTGAACT TCATCGCTCCTGACAAGCATGAGTACTGTATCTGGACGGATGGACTGAATGCGCTACTCGGGAAGGACAT GATGAGCGACCTGACGCGGAATGACCTGGACACCCTGCTCAGCATGGAAATCAAGCTCCGCCTCCTGGAC CTGGAAAACATCCAGATCCCTGACGCACCTCCGCCGATTCCCAAGGAGCCCAGCAACTATGACTTCGTCT ATGACTGTAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233105 representing NM_001206480
Red=Cloning site Green=Tags(s) MPPPADIVKVAIEWPGAYPKLMEIDQKKPLSAIIKEVCDGWSLANHEYFALQHADSSNFYITEKNRNEIK NGTILRLTTSPAQNAQQLHERIQSSSMDAKLEALKDLASLSRDVTFAQEFINLDGISLLTQMVESGTERY QKLQKIMKPCFGDMLSFTLTAFVELMDHGIVSWDTFSVAFIKKIASFVNKSAIDISILQRSLAILESMVL NSHDLYQKVAQEITIGQLIPHLQGSDQEIQTYTIAVINALFLKAPDERRQEMANILAQKQLRSIILTHVI RAQRAINNEMAHQLYVLQVLTFNLLEDRMMTKMDPQDQAQRDIIFELRRIAFDAESEPNNSSGSMEKRKS MYTRDYKKLGFINHVNPAMDFTQTPPGMLALDNMLYFAKHHQDAYIRIVLENSSREDKHECPFGRSSIEL TKMLCEILKVGELPSETCNDFHPMFFTHDRSFEEFFCICIQLLNKTWKEMRATSEDFNKVMQVVKEQVMR ALTTKPSSLDQFKSKLQNLSYTEILKIRQSERMNQEDFQSRPILELKEKIQPEILELIKQQRLNRLVEGT CFRKLNARRRQDKFWYCRLSPNHKVLHYGDLEESPQGEVPHDSLQDKLPVADIKAVVTGKDCPHMKEKGA LKQNKEVLELAFSILYDSNCQLNFIAPDKHEYCIWTDGLNALLGKDMMSDLTRNDLDTLLSMEIKLRLLD LENIQIPDAPPPIPKEPSNYDFVYDCN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001206480 |
| ORF Size | 2181 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001206480.2, NP_001193409.1 |
| RefSeq Size | 5369 bp |
| RefSeq ORF | 2184 bp |
| Locus ID | 9844 |
| UniProt ID | Q92556 |
| Protein Pathways | Chemokine signaling pathway |
| Gene Summary | This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
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