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ITGA8 (NM_003638) Human Tagged ORF Clone
CAT＃: RG218936
- TrueORF®
ITGA8 (tGFP-tagged) - Human integrin, alpha 8 (ITGA8)
ORF Plasmid: DDK
"NM_003638" in other vectors (4)
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CNY 12,450.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218936 representing NM_003638
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCCCGGGGCCAGCCGCGGTCCCCGGGGAAGCCAGGCGCCGCTGATCGCGCCCCTCTGCTGCGCCG CGGCCGCGCTGGGGATGTTGCTGTGGTCCCCCGCCTGTCAGGCGTTCAACCTGGACGTGGAAAAGCTCAC AGTGTACAGCGGCCCCAAGGGCAGCTACTTCGGCTACGCCGTGGACTTCCACATACCCGACGCCCGCACA GCGAGTGTCTTGGTGGGGGCGCCCAAAGCCAACACCAGCCAGCCCGATATCGTGGAAGGGGGAGCCGTCT ATTACTGTCCTTGGCCCGCGGAGGGGTCTGCGCAGTGCAGGCAGATACCGTTTGACACCACCAACAACAG AAAGATCAGAGTTAATGGAACCAAAGAACCTATCGAGTTCAAATCCAATCAGTGGTTTGGAGCAACAGTG AAAGCTCACAAAGGAAAAGTTGTGGCCTGTGCTCCTTTATATCACTGGAGAACTCTTAAACCGACACCAG AAAAGGACCCAGTTGGCACCTGCTATGTAGCAATTCAGAACTTCAGCGCCTATGCCGAGTTCTCTCCTTG CCGGAACAGCAATGCTGATCCGGAAGGCCAGGGTTACTGCCAAGCAGGATTTAGTCTGGATTTTTATAAG AATGGAGACCTTATTGTGGGAGGACCTGGGAGTTTCTACTGGCAAGGACAAGTGATCACTGCCAGTGTTG CAGATATCATTGCAAATTACTCATTCAAGGATATCCTCAGGAAACTGGCAGGAGAAAAGCAGACGGAAGT GGCTCCAGCTTCCTATGATGACAGTTACCTTGGATACTCAGTTGCTGCTGGGGAGTTTACTGGGGATTCT CAGCAAGAATTGGTTGCTGGAATTCCAAGAGGAGCACAGAATTTTGGATATGTTTCCATCATTAACTCTA CGGATATGACGTTTATTCAGAATTTCACGGGAGAACAGATGGCATCTTATTTTGGATATACCGTTGTCGT ATCAGATGTTAACAGTGATGGACTGGATGATGTCCTGGTTGGGGCACCTCTCTTTATGGAACGTGAATTT GAGAGCAACCCCAGAGAAGTAGGGCAAATCTACCTGTATTTGCAAGTGAGCTCTCTCCTCTTCAGAGACC CCCAGATCCTCACTGGCACCGAGACGTTTGGGAGATTCGGTAGTGCTATGGCACACTTAGGAGACCTGAA CCAAGATGGATACAATGACATTGCCATCGGAGTGCCTTTTGCAGGCAAGGATCAAAGAGGCAAAGTGCTC ATTTATAATGGGAACAAAGATGGCTTAAACACCAAGCCTTCCCAAGTTCTGCAAGGAGTGTGGGCCTCAC ATGCTGTCCCTTCCGGATTTGGCTTTACTTTAAGAGGAGATTCAGACATAGACAAGAATGATTACCCAGA TTTGATTGTGGGTGCATTTGGAACAGGAAAAGTCGCTGTTTACAGAGCAAGACCGGTTGTGACTGTAGAT GCCCAGCTTCTGCTGCACCCAATGATTATCAATCTTGAAAATAAAACTTGCCAGGTTCCAGACTCTATGA CATCTGCTGCCTGCTTTTCTTTAAGAGTATGTGCATCTGTCACAGGCCAGAGCATTGCAAACACAATAGT CTTGATGGCAGAGGTGCAATTAGATTCCCTGAAACAGAAAGGAGCTATTAAACGGACGCTCTTCCTTGAT AACCATCAGGCTCATCGCGTCTTCCCTCTTGTGATAAAAAGGCAGAAATCCCACCAGTGCCAGGATTTCA TCGTTTACCTTCGAGATGAAACTGAATTCCGAGATAAATTATCTCCAATCAACATTAGTTTGAATTACAG TTTGGACGAATCCACCTTTAAAGAAGGCCTGGAAGTGAAACCAATATTGAACTACTACAGAGAAAACATT GTTAGTGAACAGGCTCACATTCTGGTGGACTGTGGAGAAGACAATCTGTGTGTTCCTGACTTGAAGCTGT CGGCTAGACCAGATAAGCATCAGGTAATCATTGGAGATGAAAATCACCTTATGCTCATAATAAATGCAAG AAATGAAGGGGAAGGAGCATATGAAGCTGAACTCTTTGTAATGATACCAGAAGAGGCAGATTATGTTGGA ATCGAACGCAACAACAAGGGATTTCGACCACTGAGCTGTGAGTACAAGATGGAAAATGTAACCAGGATGG TGGTGTGTGACCTTGGGAACCCTATGGTGTCTGGAACAAATTATTCCCTGGGCCTCCGATTTGCAGTTCC ACGTCTTGAGAAAACAAACATGAGCATTAACTTCGATCTCCAAATCAGAAGTTCCAACAAGGACAATCCA GACAGCAATTTTGTGAGCCTGCAAATCAACATCACTGCTGTAGCGCAGGTGGAAATAAGAGGAGTGTCAC ACCCTCCGCAGATTGTTCTGCCCATTCATAACTGGGAACCAGAAGAGGAGCCCCACAAAGAGGAGGAGGT TGGACCATTGGTGGAACATATTTATGAGCTGCACAATATTGGACCAAGTACCATCAGTGACACCATCCTG GAGGTGGGCTGGCCTTTCTCTGCCCGGGATGAATTTCTTCTCTATATTTTCCATATTCAAACTCTGGGAC CTCTGCAGTGCCAACCAAATCCTAATATCAATCCACAGGATATAAAGCCTGCTGCCTCCCCAGAGGACAC CCCTGAGCTCAGCGCCTTTTTGCGAAACTCTACTATTCCTCATCTTGTCAGGAAGAGGGATGTACATGTG GTCGAATTCCACAGACAGAGCCCTGCAAAAATACTGAATTGTACAAATATCGAGTGTTTACAAATCTCCT GTGCAGTGGGACGACTCGAAGGAGGAGAAAGCGCAGTCCTGAAAGTCAGGTCACGATTATGGGCCCACAC CTTCCTCCAGAGAAAAAATGATCCCTATGCTCTTGCATCCCTGGTGTCCTTTGAAGTTAAGAAGATGCCT TATACAGATCAGCCAGCAAAACTCCCAGAAGGAAGCATAGCAATTAAGACATCAGTTATTTGGGCAACTC CGAATGTTTCCTTCTCAATCCCATTATGGGTAATAATACTAGCAATACTTCTTGGATTGTTGGTTCTCGC CATTTTAACCTTAGCTTTATGGAAGTGTGGATTCTTTGACAGAGCCAGACCTCCTCAGGAGGACATGACC GACAGGGAACAGCTGACAAATGACAAGACCCCTGAGGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218936 representing NM_003638
Red=Cloning site Green=Tags(s) MSPGASRGPRGSQAPLIAPLCCAAAALGMLLWSPACQAFNLDVEKLTVYSGPKGSYFGYAVDFHIPDART ASVLVGAPKANTSQPDIVEGGAVYYCPWPAEGSAQCRQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGATV KAHKGKVVACAPLYHWRTLKPTPEKDPVGTCYVAIQNFSAYAEFSPCRNSNADPEGQGYCQAGFSLDFYK NGDLIVGGPGSFYWQGQVITASVADIIANYSFKDILRKLAGEKQTEVAPASYDDSYLGYSVAAGEFTGDS QQELVAGIPRGAQNFGYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNSDGLDDVLVGAPLFMEREF ESNPREVGQIYLYLQVSSLLFRDPQILTGTETFGRFGSAMAHLGDLNQDGYNDIAIGVPFAGKDQRGKVL IYNGNKDGLNTKPSQVLQGVWASHAVPSGFGFTLRGDSDIDKNDYPDLIVGAFGTGKVAVYRARPVVTVD AQLLLHPMIINLENKTCQVPDSMTSAACFSLRVCASVTGQSIANTIVLMAEVQLDSLKQKGAIKRTLFLD NHQAHRVFPLVIKRQKSHQCQDFIVYLRDETEFRDKLSPINISLNYSLDESTFKEGLEVKPILNYYRENI VSEQAHILVDCGEDNLCVPDLKLSARPDKHQVIIGDENHLMLIINARNEGEGAYEAELFVMIPEEADYVG IERNNKGFRPLSCEYKMENVTRMVVCDLGNPMVSGTNYSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNP DSNFVSLQINITAVAQVEIRGVSHPPQIVLPIHNWEPEEEPHKEEEVGPLVEHIYELHNIGPSTISDTIL EVGWPFSARDEFLLYIFHIQTLGPLQCQPNPNINPQDIKPAASPEDTPELSAFLRNSTIPHLVRKRDVHV VEFHRQSPAKILNCTNIECLQISCAVGRLEGGESAVLKVRSRLWAHTFLQRKNDPYALASLVSFEVKKMP YTDQPAKLPEGSIAIKTSVIWATPNVSFSIPLWVIILAILLGLLVLAILTLALWKCGFFDRARPPQEDMT DREQLTNDKTPEA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003638 |
| ORF Size | 3189 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003638.1, NP_003629.1 |
| RefSeq Size | 3261 bp |
| RefSeq ORF | 3192 bp |
| Locus ID | 8516 |
| UniProt ID | P53708 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton |
| Gene Summary | Integrins are heterodimeric transmembrane receptor proteins that mediate numerous cellular processes including cell adhesion, cytoskeletal rearrangement, and activation of cell signaling pathways. Integrins are composed of alpha and beta subunits. This gene encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1 protein. The encoded protein is a single-pass type 1 membrane protein that contains multiple FG-GAP repeats. This repeat is predicted to fold into a beta propeller structure. This gene regulates the recruitment of mesenchymal cells into epithelial structures, mediates cell-cell interactions, and regulates neurite outgrowth of sensory and motor neurons. The integrin alpha8beta1 protein thus plays an important role in wound-healing and organogenesis. Mutations in this gene have been associated with renal hypodysplasia/aplasia-1 (RHDA1) and with several animal models of chronic kidney disease. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218936 | ITGA8 (Myc-DDK-tagged)-Human integrin, alpha 8 (ITGA8) |
CNY 7,400.00 |
|
| RC218936L3 | Lenti ORF clone of Human integrin, alpha 8 (ITGA8), Myc-DDK-tagged |
CNY 13,210.00 |
|
| RC218936L4 | Lenti ORF clone of Human integrin, alpha 8 (ITGA8), mGFP tagged |
CNY 13,210.00 |
|
| SC303358 | ITGA8 (untagged)-Human integrin, alpha 8 (ITGA8) |
CNY 7,408.00 |
