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TOPBP1 (NM_007027) Human Tagged ORF Clone
CAT＃: RG218584
- TrueORF®
TOPBP1 (tGFP-tagged) - Human topoisomerase (DNA) II binding protein 1 (TOPBP1)
ORF Plasmid: DDK
"NM_007027" in other vectors (7)
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CNY 13,300.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Dpb11; TOP2BP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218584 representing NM_007027
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGAAATGACAAAGAACCGTTTTTTGTGAAGTTTTTAAAGTCTTCAGACAATTCCAAATGTTTTT TTAAAGCTCTCGAGTCCATAAAAGAATTCCAATCAGAAGAATATCTTCAGATTATTACAGAAGAAGAGGC ATTGAAGATAAAGGAGAATGATAGATCACTTTATATCTGTGACCCTTTTAGTGGCGTTGTCTTTGATCAC CTCAAAAAGCTTGGCTGCAGAATTGTTGGTCCTCAAGTAGTCATATTTTGTATGCACCACCAGCGATGTG TCCCAAGAGCCGAACATCCAGTTTATAATATGGTTATGTCTGATGTAACCATATCTTGTACAAGTCTGGA AAAAGAAAAAAGGGAAGAAGTTCATAAATATGTACAAATGATGGGCGGACGAGTATACAGAGACCTTAAT GTATCAGTAACTCACCTTATTGCAGGAGAAGTTGGTAGCAAAAAATATTTAGTTGCTGCAAACCTGAAGA AACCTATTTTGCTTCCCTCTTGGATAAAAACACTTTGGGAGAAGTCACAAGAGAAAAAAATAACTAGATA TACTGATATAAACATGGAAGATTTCAAGTGTCCTATTTTTCTTGGTTGCATAATCTGTGTGACTGGCTTA TGTGGCTTAGACAGGAAAGAAGTTCAGCAACTCACAGTTAAGCATGGAGGTCAATACATGGGACAATTGA AAATGAATGAATGTACACACCTCATTGTGCAAGAACCAAAAGGTCAGAAGTATGAGTGTGCCAAGAGATG GAATGTACACTGTGTGACCACACAGTGGTTTTTTGACAGTATTGAGAAAGGTTTTTGTCAGGATGAATCC ATATACAAGACAGAACCTAGACCAGAAGCAAAGACTATGCCCAATTCTTCAACTCCTACCAGCCAGATCA ACACAATTGATAGTCGTACTCTTTCAGATGTCAGCAATATTTCCAACATAAATGCAAGTTGCGTAAGTGA ATCAATATGTAATTCACTTAACAGCAAACTGGAGCCTACACTTGAAAATCTAGAAAATCTGGATGTCAGT GCATTTCAAGCACCTGAAGATTTATTAGATGGTTGTCGGATATATCTTTGCGGTTTTAGTGGCAGAAAGC TAGATAAACTGAGAAGACTTATTAACAGTGGAGGTGGAGTTCGTTTTAACCAGCTAAATGAAGATGTAAC TCATGTTATTGTGGGAGATTATGATGATGAATTGAAGCAGTTTTGGAATAAATCAGCCCACAGGCCTCAT GTAGTGGGAGCAAAGTGGTTGCTAGAGTGTTTCAGTAAAGGTTATATGCTTTCTGAAGAACCATATATCC ATGCTAATTACCAGCCAGTGGAAATTCCAGTTTCACATAAGCCTGAAAGTAAAGCAGCTCTTTTAAAAAA GAAGAACAGCAGCTTCTCTAAGAAAGACTTTGCTCCTAGTGAAAAGCATGAGCAAGCTGATGAAGATCTG CTCTCTCAATATGAAAATGGTAGCTCCACAGTAGTTGAGGCTAAGACGTCTGAAGCCAGGCCCTTTAATG ATTCTACTCATGCTGAGCCCTTGAATGATTCTACTCACATTTCTTTGCAAGAAGAAAACCAGTCTTCTGT CAGTCATTGTGTCCCTGATGTTTCTACAATTACTGAAGAAGGCTTATTTAGCCAAAAGAGTTTCCTTGTT TTGGGTTTTAGTAATGAAAATGAATCTAACATCGCAAACATCATAAAAGAAAATGCTGGGAAAATCATGT CCCTTCTGAGCAGAACTGTTGCGGATTATGCTGTGGTTCCTCTGCTGGGGTGTGAAGTGGAAGCCACTGT GGGAGAAGTTGTTACAAATACATGGCTGGTTACTTGCATAGACTATCAGACTTTGTTTGATCCAAAGTCG AATCCTCTCTTCACACCAGTTCCAGTAATGACAGGAATGACTCCTTTAGAGGATTGTGTTATTTCATTTA GCCAGTGTGCTGGAGCAGAAAAAGAGTCTTTAACATTCCTAGCAAACCTCCTTGGAGCAAGTGTTCAAGA ATACTTTGTTCGCAAATCCAATGCAAAGAAAGGCATGTTTGCCAGTACTCATCTTATACTGAAAGAACGT GGTGGCTCTAAATATGAAGCTGCAAAGAAGTGGAATTTACCTGCCGTTACTATAGCTTGGCTGTTGGAGA CTGCTAGAACGGGAAAGAGAGCAGACGAAAGCCATTTTCTGATTGAAAATTCAACTAAAGAAGAACGAAG TTTGGAAACAGAAATAACAAATGGAATCAATCTAAATTCAGATACTGCAGAGCATCCTGGCACACGCCTG CAAACTCACAGAAAAACCGTCGTTACACCTTTAGATATGAACCGCTTTCAGAGTAAAGCTTTCCGTGCTG TGGTCTCACAACATGCCAGACAGGTCGCAGCCTCCCCAGCAGTAGGACAACCACTTCAGAAGGAGCCCTC GTTACACCTGGATACACCATCAAAATTCCTGTCCAAGGACAAACTCTTCAAGCCTTCCTTTGATGTGAAG GATGCACTTGCAGCCTTGGAAACTCCAGGACGTCCCAGCCAACAGAAAAGGAAACCGAGTACGCCACTCT CAGAAGTTATTGTCAAAAACTTGCAACTTGCTTTGGCAAATAGCTCTCGAAATGCTGTCGCTCTTTCTGC CAGCCCTCAACTGAAAGAGGCCCAGTCAGAGAAGGAAGAAGCCCCAAAGCCACTTCACAAAGTAGTGGTA TGTGTTAGTAAAAAACTCAGTAAGAAGCAGAGTGAACTAAATGGGATCGCAGCCTCTCTAGGAGCAGATT ACAGGTGGAGTTTTGATGAAACAGTGACTCATTTCATCTATCAAGGGCGGCCAAATGACACTAATCGGGA GTATAAATCTGTAAAAGAAAGAGGAGTACACATTGTTTCCGAGCACTGGCTTTTAGATTGTGCCCAAGAG TGTAAACATCTTCCTGAATCTCTTTATCCACATACTTATAATCCCAAAATGAGCTTGGATATCAGCGCAG TGCAAGATGGCCGGCTCTGTAATAGTCGACTACTCTCAGCTGTGTCTTCAACAAAGGATGATGAGCCAGA TCCTTTGATTTTAGAAGAAAATGATGTAGACAATATGGCCACCAATAATAAAGAGTCAGCACCATCAAAT GGAAGTGGAAAGAATGACTCTAAAGGAGTTCTGACACAGACCTTAGAGATGAGAGAGAACTTTCAGAAGC AGTTACAGGAGATAATGTCTGCAACATCAATAGTGAAACCCCAAGGGCAGAGGACTTCCCTTTCAAGAAG TGGTTGTAACAGCGCATCTTCAACCCCTGACAGCACTCGCTCTGCTCGCAGTGGACGAAGTAGAGTCCTA GAGGCACTGAGGCAGTCTCGTCAGACAGTACCTGATGTCAACACAGAGCCTTCCCAAAATGAACAGATCA TTTGGGATGACCCTACAGCAAGGGAGGAGAGAGCAAGGCTTGCCAGCAATTTGCAGTGGCCTAGTTGTCC CACACAATACTCTGAGCTTCAGGTTGACATTCAAAACTTGGAGGATTCTCCTTTTCAAAAGCCTTTACAT GATTCAGAAATTGCTAAACAGGCTGTCTGTGATCCTGGAAACATACGTGTGACTGAAGCTCCCAAACACC CAATCTCTGAAGAACTGGAAACTCCCATAAAAGACAGCCACCTGATCCCTACGCCTCAAGCCCCCAGTAT TGCCTTTCCACTCGCCAACCCCCCTGTGGCTCCGCACCCTAGAGAAAAGATTATAACGATAGAGGAGACT CATGAAGAATTAAAAAAACAGTACATATTTCAGTTATCATCTCTGAATCCTCAAGAACGTATTGACTATT GTCATCTGATTGAGAAACTAGGTGGATTGGTGATAGAAAAGCAGTGCTTTGATCCCACCTGTACACACAT TGTTGTGGGACATCCACTTCGAAACGAGAAGTATTTAGCCTCAGTGGCAGCTGGGAAGTGGGTGCTTCAT CGCTCCTACCTTGAAGCCTGCAGGACTGCTGGACACTTCGTGCAGGAAGAAGACTATGAATGGGGAAGTA GTTCCATACTTGATGTTCTGACTGGAATCAATGTACAGCAACGAAGACTAGCACTTGCAGCAATGAGATG GAGAAAAAAAATCCAGCAAAGACAAGAATCTGGCATTGTTGAGGGAGCATTTAGTGGGTGGAAGGTTATT TTACATGTGGATCAGTCTCGAGAAGCAGGCTTCAAACGCCTTCTTCAGTCAGGAGGAGCAAAGGTGCTAC CTGGTCATTCTGTACCTTTATTTAAAGAGGCCACACATCTTTTTTCTGACTTGAATAAACTGAAACCAGA TGACTCAGGAGTTAATATAGCAGAAGCTGCTGCCCAGAACGTGTACTGCTTGAGAACAGAATACATTGCT GATTATCTCATGCAGGAATCACCTCCTCATGTAGAAAATTACTGTCTACCAGAAGCTATTTCATTTATTC AGAATAATAAGGAACTTGGGACTGGATTATCACAAAAGAGGAAAGCTCCTACAGAAAAAAATAAAATCAA ACGACCTAGAGTACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218584 representing NM_007027
Red=Cloning site Green=Tags(s) MSRNDKEPFFVKFLKSSDNSKCFFKALESIKEFQSEEYLQIITEEEALKIKENDRSLYICDPFSGVVFDH LKKLGCRIVGPQVVIFCMHHQRCVPRAEHPVYNMVMSDVTISCTSLEKEKREEVHKYVQMMGGRVYRDLN VSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITRYTDINMEDFKCPIFLGCIICVTGL CGLDRKEVQQLTVKHGGQYMGQLKMNECTHLIVQEPKGQKYECAKRWNVHCVTTQWFFDSIEKGFCQDES IYKTEPRPEAKTMPNSSTPTSQINTIDSRTLSDVSNISNINASCVSESICNSLNSKLEPTLENLENLDVS AFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIVGDYDDELKQFWNKSAHRPH VVGAKWLLECFSKGYMLSEEPYIHANYQPVEIPVSHKPESKAALLKKKNSSFSKKDFAPSEKHEQADEDL LSQYENGSSTVVEAKTSEARPFNDSTHAEPLNDSTHISLQEENQSSVSHCVPDVSTITEEGLFSQKSFLV LGFSNENESNIANIIKENAGKIMSLLSRTVADYAVVPLLGCEVEATVGEVVTNTWLVTCIDYQTLFDPKS NPLFTPVPVMTGMTPLEDCVISFSQCAGAEKESLTFLANLLGASVQEYFVRKSNAKKGMFASTHLILKER GGSKYEAAKKWNLPAVTIAWLLETARTGKRADESHFLIENSTKEERSLETEITNGINLNSDTAEHPGTRL QTHRKTVVTPLDMNRFQSKAFRAVVSQHARQVAASPAVGQPLQKEPSLHLDTPSKFLSKDKLFKPSFDVK DALAALETPGRPSQQKRKPSTPLSEVIVKNLQLALANSSRNAVALSASPQLKEAQSEKEEAPKPLHKVVV CVSKKLSKKQSELNGIAASLGADYRWSFDETVTHFIYQGRPNDTNREYKSVKERGVHIVSEHWLLDCAQE CKHLPESLYPHTYNPKMSLDISAVQDGRLCNSRLLSAVSSTKDDEPDPLILEENDVDNMATNNKESAPSN GSGKNDSKGVLTQTLEMRENFQKQLQEIMSATSIVKPQGQRTSLSRSGCNSASSTPDSTRSARSGRSRVL EALRQSRQTVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSCPTQYSELQVDIQNLEDSPFQKPLH DSEIAKQAVCDPGNIRVTEAPKHPISEELETPIKDSHLIPTPQAPSIAFPLANPPVAPHPREKIITIEET HEELKKQYIFQLSSLNPQERIDYCHLIEKLGGLVIEKQCFDPTCTHIVVGHPLRNEKYLASVAAGKWVLH RSYLEACRTAGHFVQEEDYEWGSSSILDVLTGINVQQRRLALAAMRWRKKIQQRQESGIVEGAFSGWKVI LHVDQSREAGFKRLLQSGGAKVLPGHSVPLFKEATHLFSDLNKLKPDDSGVNIAEAAAQNVYCLRTEYIA DYLMQESPPHVENYCLPEAISFIQNNKELGTGLSQKRKAPTEKNKIKRPRVH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007027 |
| ORF Size | 4566 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007027.4 |
| RefSeq Size | 5378 bp |
| RefSeq ORF | 4569 bp |
| Locus ID | 11073 |
| UniProt ID | Q92547 |
| Domains | BRCT |
| Gene Summary | This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218584 | TOPBP1 (Myc-DDK-tagged)-Human topoisomerase (DNA) II binding protein 1 (TOPBP1) |
CNY 15,616.00 |
|
| RC218584L1 | Lenti-ORF clone of TOPBP1 (Myc-DDK-tagged)-Human topoisomerase (DNA) II binding protein 1 (TOPBP1) |
CNY 18,016.00 |
|
| RC218584L2 | Lenti-ORF clone of TOPBP1 (mGFP-tagged)-Human topoisomerase (DNA) II binding protein 1 (TOPBP1) |
CNY 13,970.00 |
|
| RC218584L3 | Lenti-ORF clone of TOPBP1 (Myc-DDK-tagged)-Human topoisomerase (DNA) II binding protein 1 (TOPBP1) |
CNY 13,970.00 |
|
| RC218584L4 | Lenti-ORF clone of TOPBP1 (mGFP-tagged)-Human topoisomerase (DNA) II binding protein 1 (TOPBP1) |
CNY 13,970.00 |
|
| SC115748 | TOPBP1 (untagged)-Human topoisomerase (DNA) II binding protein 1 (TOPBP1) |
CNY 14,736.00 |
|
| SC328077 | TOPBP1 (untagged)-Human topoisomerase (DNA) II binding protein 1 (TOPBP1) |
CNY 10,450.00 |
