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PRB1 (NM_005039) Human Tagged ORF Clone
CAT＃: RG218178
- TrueORF®
PRB1 (tGFP-tagged) - Human proline-rich protein BstNI subfamily 1 (PRB1), transcript variant 1
ORF Plasmid: DDK
"NM_005039" in other vectors (2)
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CNY 4,370.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PM; PMF; PMS; PRB1L; PRB1M |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218178 representing NM_005039
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTTGATTCTGCTGTCAGTGGCCTTGCTGGCCCTGAGCTCAGCTCAGAACTTAAATGAAGATGTCA GCCAGGAAGAATCTCCCTCCCTAATAGCAGGAAATCCACAAGGACCATCCCCACAAGGAGGCAACAAGCC CCAAGGCCCCCCACCTCCTCCAGGAAAGCCACAAGGACCACCCCCACAAGGAGGCAACAAACCTCAAGGT CCCCCACCTCCAGGAAAGCCACAAGGACCACCCCCACAAGGGGACAAGTCCCGAAGTCCCCGATCTCCTC CAGGAAAACCACAAGGACCACCCCCACAAGGAGGTAACCAGCCCCAAGGTCCCCCACCTCCTCCAGGAAA GCCACAAGGACCACCCCCACAAGGAGGCAACAGACCTCAAGGTCCCCCACCTCCAGGAAAGCCACAAGGA CCACCCCCACAAGGAGACAAGTCCCGAAGTCCCCGATCTCCTCCAGGAAAGCCACAAGGACCACCCCCAC AAGGAGGTAACCAACCCCAAGGTCCCCCACCTCCTCCAGGAAAGCCACAAGGACCACCCCCACAAGGAGG CAAGAAACCTCAGGGTCCCCCACCTCCAGGAAAGCCACAAGGACCACCCCCACAAGGAGACAAGTCCCGA AGTTCCCAATCTCCTCCAGGAAAGCCACAAGGACCACCCCCACAAGGAGGCAACCAGCCCCAAGGTCCCC CACCTCCTCCAGGAAAGCCACAAGGACCACCCCCACAAGGAGGCAACAAACCTCAAGGTCCCCCACCTCC AGGAAAGCCACAAGGACCACCCGCACAAGGAGGCAGCAAGTCCCAAAGTGCCCGATCTCCTCCAGGAAAG CCACAAGGACCACCCCAACAAGAAGGCAACAATCCTCAAGGTCCCCCACCTCCAGCAGGAGGCAATCCCC AGCAGCCTCAGGCACCTCCTGCTGGACAGCCCCAGGGACCACCACGCCCTCCTCAAGGGGGCAGACCTTC CAGACCTCCCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218178 representing NM_005039
Red=Cloning site Green=Tags(s) MLLILLSVALLALSSAQNLNEDVSQEESPSLIAGNPQGPSPQGGNKPQGPPPPPGKPQGPPPQGGNKPQG PPPPGKPQGPPPQGDKSRSPRSPPGKPQGPPPQGGNQPQGPPPPPGKPQGPPPQGGNRPQGPPPPGKPQG PPPQGDKSRSPRSPPGKPQGPPPQGGNQPQGPPPPPGKPQGPPPQGGKKPQGPPPPGKPQGPPPQGDKSR SSQSPPGKPQGPPPQGGNQPQGPPPPPGKPQGPPPQGGNKPQGPPPPGKPQGPPAQGGSKSQSARSPPGK PQGPPQQEGNNPQGPPPPAGGNPQQPQAPPAGQPQGPPRPPQGGRPSRPPQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005039 |
| ORF Size | 993 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005039.2, NP_005030.2 |
| RefSeq Size | 1173 bp |
| RefSeq ORF | 996 bp |
| Locus ID | 5542 |
| UniProt ID | P04280 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the heterogeneous family of basic, proline-rich, human salivary glycoproteins. The encoded preproprotein undergoes proteolytic processing to generate one or more mature peptides before secretion from the parotid glands. Multiple alleles of this gene exhibiting variations in the length of the tandem repeats have been identified. The reference genome encodes the "Medium" allele. This gene is located in a cluster of closely related salivary proline-rich proteins on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Nov 2015] |
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