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NLRC5 (NM_032206) Human Tagged ORF Clone
CAT＃: RG214810
- TrueORF®
NLRC5 (tGFP-tagged) - Human NLR family, CARD domain containing 5 (NLRC5)
ORF Plasmid: DDK
"NM_032206" in other vectors (3)
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CNY 20,520.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CLR16.1; NOD4; NOD27 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214810 representing NM_032206
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCCGTTGGCCTCCAGCTCGGCAACAAGAACCTGTGGAGCTGTCTTGTGAGGCTGCTCACCAAAG ACCCAGAATGGCTGAACGCCAAGATGAAGTTCTTCCTCCCCAACACGGACCTGGATTCCAGGAACGAGAC CTTGGACCCTGAACAGAGAGTCATCCTGCAACTCAACAAGCTGCATGTCCAGGGTTCGGACACCTGGCAG TCTTTCATTCATTGTGTGTGCATGCAGCTGGAGGTGCCTCTGGACCTGGAGGTGCTGCTGCTGAGTACTT TTGGCTATGATGATGGGTTCACCAGCCAGCTGGGAGCTGAGGGGAAAAGCCAACCTGAATCTCAGCTCCA CCATGGCCTGAAGCGCCCACATCAGAGCTGTGGGTCCTCACCCCGCCGGAAGCAGTGCAAGAAGCAGCAG CTAGAGTTGGCCAAGAAGTACCTGCAGCTCCTGCGGACCTCTGCCCAGCAGCGCTACAGGAGCCAAATCC CTGGGTCAGGGCAGCCCCACGCCTTCCACCAGGTCTATGTCCCTCCAATCCTGCGCCGGGCCACAGCATC CTTAGACACTCCGGAGGGGGCCATTATGGGGGACGTCAAGGTGGAAGATGGTGCTGACGTGAGCATCTCG GACCTCTTCAACACCAGGGTTAACAAGGGCCCGAGGGTGACCGTGCTTTTGGGGAAGGCTGGCATGGGCA AGACCACGCTGGCCCACCGGCTCTGCCAGAAGTGGGCAGAGGGCCATCTGAACTGTTTCCAGGCCCTGTT CCTTTTTGAATTCCGCCAGCTCAACTTGATCACGAGGTTCCTGACACCGTCCGAGCTCCTTTTTGATCTG TACCTGAGCCCTGAATCGGACCACGACACTGTCTTCCAGTACCTGGAGAAGAACGCTGACCAAGTCCTGC TGATCTTTGATGGGCTAGATGAGGCCCTCCAGCCTATGGGTCCTGATGGCCCAGGCCCAGTCCTCACCCT TTTCTCCCATCTCTGCAATGGGACCCTCCTGCCTGGCTGCCGGGTGATGGCTACCTCCCGTCCAGGGAAG CTGCCTGCCTGCCTGCCTGCAGAGGCAGCCATGGTCCACATGTTGGGCTTTGATGGGCCACGGGTGGAAG AATATGTGAATCACTTCTTCAGCGCCCAGCCATCGCGGGAGGGGGCCCTGGTGGAGTTACAGACAAATGG ACGTCTCCGAAGCCTGTGTGCGGTGCCCGCACTGTGCCAAGTCGCCTGTCTCTGCCTCCACCATCTGCTT CCTGACCACGCCCCAGGCCAGTCTGTGGCCCTCCTGCCCAACATGACTCAGCTCTATATGCAGATGGTGC TCGCCCTCAGCCCCCCTGGGCACTTGCCCACCTCGTCCCTACTGGACCTGGGGGAGGTGGCCCTGAGGGG CCTGGAGACAGGGAAGGTTATCTTCTATGCAAAAGATATTGCTCCACCCTTGATAGCTTTTGGGGCCACT CACAGCCTGCTGACTTCCTTCTGCGTCTGCACAGGCCCTGGGCACCAGCAGACAGGCTATGCTTTCACCC ACCTCAGCCTGCAGGAGTTTCTTGCTGCCCTGCACCTGATGGCCAGCCCCAAGGTGAACAAAGACACACT TACCCAGTATGTTACCCTCCATTCCCGCTGGGTACAGCGGACCAAAGCTAGACTGGGCCTCTCAGACCAC CTCCCCACCTTCCTGGCGGGCCTGGCATCCTGCACCTGCCGCCCCTTCCTTAGCCACCTGGCGCAGGGCA ATGAGGACTGTGTGGGTGCCAAGCAGGCTGCTGTAGTGCAGGTGTTGAAGAAGTTGGCCACCCGCAAGCT CACAGGGCCAAAGGTTGTAGAGCTGTGTCACTGTGTGGATGAGACACAGGAGCCTGAGCTGGCCAGTCTC ACCGCACAAAGCCTCCCCTATCAACTGCCCTTCCACAATTTCCCACTGACCTGCACCGACCTGGCCACCC TGACCAACATCCTAGAGCACAGGGAGGCCCCCATCCACCTGGATTTTGATGGCTGTCCCCTGGAGCCCCA CTGCCCTGAGGCTCTGGTAGGCTGTGGGCAGATAGAGAATCTCAGCTTTAAGAGCAGGAAGTGTGGGGAT GCCTTTGCAGAAGCCCTCTCCAGGAGCTTGCCGACAATGGGGAGGCTGCAGATGCTGGGGTTAGCAGGAA GTAAAATCACTGCCCGAGGCATCAGCCACCTGGTGAAAGCTTTGCCTCTCTGTCCACAGCTGAAAGAAGT CAGTTTTCGGGACAACCAGCTCAGTGACCAGGTGGTGCTGAACATTGTGGAGGTTCTCCCTCACCTACCA CGGCTCCGGAAGCTTGACCTGAGCAGCAACAGCATCTGCGTGTCAACCCTACTCTGCTTGGCAAGGGTGG CAGTCACGTGTCCTACCGTCAGGATGCTTCAGGCCAGGGAGGCGGACCTCATCTTCCTTCTTTCCCCGCC CACAGAGACAACTGCAGAGCTACAAAGAGCTCCAGACCTGCAGGAAAGTGACGGCCAGAGGAAAGGGGCT CAGAGCAGAAGCTTGACGCTCAGGCTGCAGAAGTGTCAGCTCCAGGTCCGTGATGCGGAGGCCCTCATAG CCCTGCTCCAGGAAGGCCCTCACCTGGAGGAAGTGGACCTCTCAGGGAACCAGCTGGAAGATGAAGGCTG TCGGCTGATGGCAGAGGCTGCATCCCAGCTGCACATCGCCAGGAAGCTGGACCTCAGTGACAACGGGCTT TCTGTGGCCGGGGTGCATTGTGTGCTGAGGGCCGTGAGTGCGTGCTGGACCCTGGCAGAGCTGCACATCA GCCTGCAGCACAAAACTGTGATCTTCATGTTTGCCCAGGAGCCAGAGGAGCAGAAGGGGCCCCAGGAGAG GGCTGCATTTCTTGACAGCCTCATGCTCCAGATGCCCTCTGAGCTGCCTCTGAGCTCCCGAAGGATGAGG CTGACACATTGTGGCCTCCAAGAAAAGCACCTAGAGCAGCTCTGCAAGGCTCTGGGAGGAAGCTGCCACC TCGGTCACCTCCACCTCGACTTCTCAGGCAATGCTCTGGGGGATGAAGGTGCAGCCCGGCTGGCTCAGCT GCTCCCAGGGCTGGGAGCTCTGCAGTCCTTGAACCTCAGTGAGAACGGTTTGTCCCTGGATGCCGTGTTG GGTTTGGTTCGGTGCTTCTCCACTCTGCAGTGGCTCTTCCGCTTGGACATCAGCTTTGAAAGCCAACACA TCCTCCTGAGAGGGGACAAGACAAGCAGGGATATGTGGGCCACTGGATCTTTGCCAGACTTCCCAGCTGC AGCCAAGTTCTTAGGGTTCCGTCAGCGCTGCATCCCCAGGAGCCTCTGCCTCAGTGAGTGTCCTCTGGAG CCCCCAAGCCTCACCCGCCTCTGTGCCACTCTGAAGGACTGCCCGGGACCCCTGGAACTGCAATTGTCCT GTGAGTTCCTGAGTGACCAGAGCCTGGAGACTCTACTGGACTGCTTACCTCAACTCCCTCAGCTGAGCCT GCTGCAGCTGAGCCAGACGGGACTGTCCCCGAAAAGCCCCTTCCTGCTGGCCAACACCTTAAGCCTGTGT CCACGGGTTAAAAAGGTGGATCTCAGGTCCCTGCACCATGCAACTTTGCACTTCAGATCCAACGAGGAGG AGGAAGGCGTGTGCTGTGGCAGGTTCACAGGCTGCAGCCTCAGCCAGGAGCACGTAGAGTCACTCTGCTG GTTGCTGAGCAAGTGTAAAGACCTCAGCCAGGTGGATCTCTCAGCAAACCTGCTGGGCGACAGCGGACTC AGATGCCTTCTGGAATGTCTGCCGCAGGTGCCCATCTCCGGTTTGCTTGATCTGAGTCACAACAGCATTT CTCAGGAAAGTGCCCTGTACCTGCTGGAGACACTGCCCTCCTGCCCACGTGTCCGGGAGGCCTCAGTGAA CCTGGGCTCTGAGCAGAGCTTCCGGATTCACTTCTCCAGAGAGGACCAGGCTGGGAAGACACTCAGGCTA AGTGAGTGCAGCTTCCGGCCAGAGCACGTGTCCAGGCTGGCCACCGGCTTGAGCAAGTCCCTGCAGCTGA CGGAGCTCACGCTGACCCAGTGCTGCCTGGGCCAGAAGCAGCTGGCCATCCTCCTGAGCTTGGTGGGGCG ACCCGCAGGGCTGTTCAGCCTCAGGGTGCAGGAGCCGTGGGCGGACAGAGCCAGGGTTCTCTCCCTGTTA GAAGTCTGCGCCCAGGCCTCAGGCAGTGTCACTGAAATCAGCATCTCCGAGACCCAGCAGCAGCTCTGTG TCCAGCTGGAATTTCCTCGCCAGGAAGAGAATCCAGAAGCTGTGGCACTCAGGTTGGCTCACTGTGACCT TGGAGCCCACCACAGCCTTCTTGTCGGGCAGCTGATGGAGACATGTGCCAGGCTGCAGCAGCTCAGCTTG TCTCAGGTTAACCTCTGTGAGGACGATGATGCCAGTTCCCTGCTGCTGCAGAGCCTCCTGCTGTCCCTCT CTGAGCTGAAGACATTTCGGCTGACCTCCAGCTGTGTGAGCACCGAGGGCCTCGCCCACCTGGCATCTGG TCTGGGCCACTGCCACCACTTGGAGGAGCTGGACTTGTCTAACAATCAATTTGATGAGGAGGGCACCAAG GCGCTGATGAGGGCCCTTGAGGGGAAATGGATGCTAAAGAGGCTGGACCTCAGTCACCTTCTGCTGAACA GCTCCACCTTGGCCTTGCTTACTCACAGACTAAGCCAGATGACCTGCCTGCAGAGCCTCAGACTGAACAG GAACAGTATCGGTGATGTCGGTTGCTGCCACCTTTCTGAGGCTCTCAGGGCTGCCACCAGCCTAGAGGAG CTGGACTTGAGCCACAACCAGATTGGAGACGCTGGTGTCCAGCACTTAGCTACCATCCTGCCTGGGCTGC CAGAGCTCAGGAAGATAGACCTCTCAGGGAATAGCATCAGCTCAGCCGGGGGAGTGCAGTTGGCAGAGTC TCTCGTTCTTTGCAGGCGCCTGGAGGAGTTGATGCTTGGCTGCAATGCCCTGGGGGATCCCACAGCCCTG GGGCTGGCTCAGGAGCTGCCCCAGCACCTGAGGGTCCTACACCTACCATTCAGCCATCTGGGCCCAGGTG GGGCCCTGAGCCTGGCCCAGGCCCTGGATGGATCCCCCCATTTGGAAGAGATCAGCTTGGCGGAAAACAA CCTGGCTGGAGGGGTCCTGCGTTTCTGTATGGAGCTCCCGCTGCTCAGACAGATAGACCTGGTTTCCTGT AAGATTGACAACCAGACTGCCAAGCTCCTCACCTCCAGCTTCACGAGCTGCCCTGCCCTGGAAGTAATCT TGCTGTCCTGGAATCTCCTCGGGGATGAGGCAGCTGCCGAGCTGGCCCAGGTGCTGCCGCAGATGGGCCG GCTGAAGAGAGTGGACCTGGAGAAGAATCAGATCACAGCTTTGGGGGCCTGGCTCCTGGCTGAAGGACTG GCCCAGGGGTCTAGCATCCAAGTCATCCGCCTCTGGAATAACCCCATTCCCTGCGACATGGCCCAGCACC TGAAGAGCCAGGAGCCCAGGCTGGACTTTGCCTTCTTTGACAACCAGCCCCAGGCCCCTTGGGGTACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214810 representing NM_032206
Red=Cloning site Green=Tags(s) MDPVGLQLGNKNLWSCLVRLLTKDPEWLNAKMKFFLPNTDLDSRNETLDPEQRVILQLNKLHVQGSDTWQ SFIHCVCMQLEVPLDLEVLLLSTFGYDDGFTSQLGAEGKSQPESQLHHGLKRPHQSCGSSPRRKQCKKQQ LELAKKYLQLLRTSAQQRYRSQIPGSGQPHAFHQVYVPPILRRATASLDTPEGAIMGDVKVEDGADVSIS DLFNTRVNKGPRVTVLLGKAGMGKTTLAHRLCQKWAEGHLNCFQALFLFEFRQLNLITRFLTPSELLFDL YLSPESDHDTVFQYLEKNADQVLLIFDGLDEALQPMGPDGPGPVLTLFSHLCNGTLLPGCRVMATSRPGK LPACLPAEAAMVHMLGFDGPRVEEYVNHFFSAQPSREGALVELQTNGRLRSLCAVPALCQVACLCLHHLL PDHAPGQSVALLPNMTQLYMQMVLALSPPGHLPTSSLLDLGEVALRGLETGKVIFYAKDIAPPLIAFGAT HSLLTSFCVCTGPGHQQTGYAFTHLSLQEFLAALHLMASPKVNKDTLTQYVTLHSRWVQRTKARLGLSDH LPTFLAGLASCTCRPFLSHLAQGNEDCVGAKQAAVVQVLKKLATRKLTGPKVVELCHCVDETQEPELASL TAQSLPYQLPFHNFPLTCTDLATLTNILEHREAPIHLDFDGCPLEPHCPEALVGCGQIENLSFKSRKCGD AFAEALSRSLPTMGRLQMLGLAGSKITARGISHLVKALPLCPQLKEVSFRDNQLSDQVVLNIVEVLPHLP RLRKLDLSSNSICVSTLLCLARVAVTCPTVRMLQAREADLIFLLSPPTETTAELQRAPDLQESDGQRKGA QSRSLTLRLQKCQLQVRDAEALIALLQEGPHLEEVDLSGNQLEDEGCRLMAEAASQLHIARKLDLSDNGL SVAGVHCVLRAVSACWTLAELHISLQHKTVIFMFAQEPEEQKGPQERAAFLDSLMLQMPSELPLSSRRMR LTHCGLQEKHLEQLCKALGGSCHLGHLHLDFSGNALGDEGAARLAQLLPGLGALQSLNLSENGLSLDAVL GLVRCFSTLQWLFRLDISFESQHILLRGDKTSRDMWATGSLPDFPAAAKFLGFRQRCIPRSLCLSECPLE PPSLTRLCATLKDCPGPLELQLSCEFLSDQSLETLLDCLPQLPQLSLLQLSQTGLSPKSPFLLANTLSLC PRVKKVDLRSLHHATLHFRSNEEEEGVCCGRFTGCSLSQEHVESLCWLLSKCKDLSQVDLSANLLGDSGL RCLLECLPQVPISGLLDLSHNSISQESALYLLETLPSCPRVREASVNLGSEQSFRIHFSREDQAGKTLRL SECSFRPEHVSRLATGLSKSLQLTELTLTQCCLGQKQLAILLSLVGRPAGLFSLRVQEPWADRARVLSLL EVCAQASGSVTEISISETQQQLCVQLEFPRQEENPEAVALRLAHCDLGAHHSLLVGQLMETCARLQQLSL SQVNLCEDDDASSLLLQSLLLSLSELKTFRLTSSCVSTEGLAHLASGLGHCHHLEELDLSNNQFDEEGTK ALMRALEGKWMLKRLDLSHLLLNSSTLALLTHRLSQMTCLQSLRLNRNSIGDVGCCHLSEALRAATSLEE LDLSHNQIGDAGVQHLATILPGLPELRKIDLSGNSISSAGGVQLAESLVLCRRLEELMLGCNALGDPTAL GLAQELPQHLRVLHLPFSHLGPGGALSLAQALDGSPHLEEISLAENNLAGGVLRFCMELPLLRQIDLVSC KIDNQTAKLLTSSFTSCPALEVILLSWNLLGDEAAAELAQVLPQMGRLKRVDLEKNQITALGAWLLAEGL AQGSSIQVIRLWNNPIPCDMAQHLKSQEPRLDFAFFDNQPQAPWGT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032206 |
| ORF Size | 5598 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032206.2, NP_115582.2 |
| RefSeq Size | 5601 bp |
| RefSeq ORF | 5601 bp |
| Locus ID | 84166 |
| UniProt ID | Q86WI3 |
| Domains | LRR, LRR_RI, LRR_CC, LRR_PS |
| Gene Summary | This gene encodes a member of the caspase recruitment domain-containing NLR family. This gene plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways. [provided by RefSeq, Oct 2011] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214810 | NLRC5 (Myc-DDK-tagged)-Human NLR family, CARD domain containing 5 (NLRC5) |
CNY 12,544.00 |
|
| RC214810L1 | Lenti ORF clone of Human NLR family, CARD domain containing 5 (NLRC5), Myc-DDK-tagged |
CNY 14,944.00 |
|
| SC308759 | NLRC5 (untagged)-Human NLR family, CARD domain containing 5 (NLRC5) |
CNY 12,552.00 |
