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WNK3 (NM_020922) Human Tagged ORF Clone
CAT＃: RG214627
- TrueORF®
WNK3 (tGFP-tagged) - Human WNK lysine deficient protein kinase 3 (WNK3), transcript variant 1
ORF Plasmid: DDK
"NM_020922" in other vectors (2)
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CNY 19,760.00
货期*
4周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PRKWNK3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214627 representing NM_020922
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTGATTCAGGGGATCCAGCCAGCACAGAAGATTCTGAGAAACCTGATGGAATTTCATTTGAAA ACAGAGTTCCCCAGGTCGCTGCAACTTTGACAGTAGAAGCTAGACTAAAGGAGAAAAACAGTACCTTCTC TGCTTCTGGGGAAACTGTAGAAAGGAAGAGATTTTTCCGAAAGAGTGTTGAAATGACGGAAGATGACAAA GTTGCCGAATCATCCCCCAAAGATGAGAGAATTAAGGCTGCAATGAATATTCCAAGAGTAGATAAGCTTC CTTCAAATGTGTTGAGAGGTGGACAAGAAGTTAAATATGAACAGTGTTCAAAGTCAACCTCAGAAATCTC AAAAGATTGTTTCAAGGAGAAAAATGAAAAGGAAATGGAAGAAGAAGCAGAAATGAAGGCTGTAGCTACT TCTCCTAGTGGCAGATTCCTGAAATTTGACATAGAACTAGGAAGAGGAGCATTTAAAACAGTATATAAAG GACTGGACACTGAAACATGGGTTGAGGTTGCTTGGTGTGAGCTGCAGGACCGAAAGTTAACCAAAGCTGA GCAGCAAAGATTCAAGGAAGAAGCAGAGATGTTGAAGGGTCTCCAGCACCCCAATATAGTTCGATTTTAT GATTCCTGGGAATCTATATTAAAAGGAAAGAAATGTATTGTATTAGTGACTGAACTAATGACATCTGGGA CCTTAAAGACGTACTTAAAACGATTTAAAGTCATGAAACCAAAGGTCTTAAGGAGCTGGTGCAGGCAAAT TTTAAAGGGGTTGCAGTTCTTGCACACTAGGACTCCTCCTATTATTCACCGGGATCTGAAGTGTGACAAT ATTTTCATCACGGGACCCACTGGATCTGTGAAGATTGGTGATCTAGGATTAGCCACCTTAATGCGTACCT CATTTGCTAAGAGTGTCATTGGAACTCCTGAGTTTATGGCTCCAGAGATGTATGAAGAACACTATGATGA ATCCGTAGATGTTTATGCTTTTGGAATGTGTATGCTGGAAATGGCCACATCGGAGTATCCTTATTCTGAG TGTCAGAATGCAGCTCAAATATACCGGAAAGTAACTAGTGGCATAAAACCAGCCAGCTTCAATAAAGTCA CTGATCCTGAAGTCAAAGAAATCATTGAAGGATGTATTCGTCAAAACAAATCTGAAAGGTTGTCTATCAG GGACCTATTAAACCATGCATTTTTTGCTGAGGATACAGGACTGAGGGTGGAGTTAGCAGAAGAAGATGAT TGCTCAAATTCATCCCTTGCTTTAAGACTCTGGGTTGAAGACCCTAAAAAATTGAAAGGCAAACACAAAG ACAATGAAGCTATTGAATTTAGTTTCAACTTAGAAACAGATACACCTGAGGAAGTAGCATATGAAATGGT CAAGTCTGGGTTCTTCCATGAAAGTGATTCCAAAGCTGTTGCTAAATCCATTAGAGACCGGGTGACGCCA ATAAAGAAGACAAGAGAGAAGAAGCCTGCTGGCTGTTTGGAAGAACGCAGGGATTCTCAGTGCAAGTCTA TGGGGAATGTATTCCCTCAGCCCCAGAATACAACTTTACCCCTTGCTCCCGCTCAGCAAACTGGGGCTGA ATGTGAAGAAACTGAAGTTGATCAACATGTTAGACAACAGCTTCTACAAAGAAAACCACAGCAGCACTGC TCCTCTGTTACAGGTGACAATTTGTCTGAGGCAGGAGCTGCATCAGTTATACATTCAGATACTTCAAGTC AGCCCAGTGTAGCCTATTCCTCAAATCAAACGATGGGCTCTCAAATGGTTTCTAATATCCCGCAGGCTGA AGTAAATGTTCCAGGGCAAATTTATTCATCTCAGCAACTAGTAGGACATTACCAGCAAGTTTCAGGGTTA CAGAAGCATTCAAAGCTGACTCAGCCGCAGATTTTGCCTTTGGTTCAAGGTCAGTCCACTGTTTTACCTG TACATGTCCTTGGACCGACAGTTGTTTCACAACCCCAGGTTTCCCCATTAACTGTTCAGAAGGTCCCACA GATAAAGCCTGTATCCCAACCAGTTGGAGCTGAACAACAAGCAGCTCTTCTAAAACCAGATTTAGTTCGA AGCTTGAATCAAGATGTGGCAACTACGAAGGAAAACGTCAGTAGCCCTGATAACCCAAGTGGAAATGGCA AACAGGATCGGATCAAACAGAGAAGAGCTTCCTGTCCCCGACCAGAGAAGGGGACTAAATTTCAGCTTAC CGTCCTTCAGGTATCAACCTCTGGAGATAACATGGTAGAGTGTCAGCTGGAGACACACAACAACAAGATG GTCACCTTCAAGTTTGATGTTGATGGTGATGCGCCAGAGGATATTGCAGACTATATGGTTGAAGATAACT TTGTGCTGGAAAGTGAGAAAGAAAAATTTGTAGAAGAATTGAGAGCTATTGTAGGTCAAGCCCAGGAGAT CCTTCATGTCCACTTTGCCACAGAAAGAGCCACTGGAGTTGATTCTATTACTGTGGACTCCAACAGTAGC CAGACAGGGTCATCTGAACAAGTACAGATAAATTCTACATCTACTCAAACCAGCAATGAATCTGCTCCTC AGTCATCCCCAGTTGGTCGGTGGCGATTCTGTATCAATCAGACGATAAGAAACCGTGAGACTCAGTCTCC TCCTTCTCTTCAGCATTCCATGTCTGCGGTTCCTGGCCGACATCCACTTCCTAGTCCAAAAAACACAAGT AATAAGGAAATATCACGGGACACATTGCTCACTATAGAAAATAATCCATGCCACCGTGCACTTTTCACCT CCAAATCAGAACACAAGGATGTGGTTGATGGTAAGATTTCTGAATGTGCTAGTGTAGAAACCAAGCAGCC AGCTATACTTTATCAAGTGGAAGATAACAGGCAGATAATGGCACCAGTTACTAATAGTTCCAGTTACTCT ACTACTTCAGTTCGTGCAGTTCCAGCTGAATGTGAGGGACTCACCAAACAAGCAAGCATATTCATACCTG TGTATCCATGTCACCAAACTGCCAGTCAGGCTGATGCACTTATGTCCCATCCTGGCGAATCAACTCAGAC TTCTGGTAACTCTCTTACAACTCTGGCATTTGATCAAAAGCCTCAAACCTTATCAGTACAGCAGCCAGCT ATGGATGCAGAGTTTATTTCTCAAGAAGGAGAAACTACAGTGAACACTGAAGCAAGTTCTCCTAAGACAG TCATTCCCACTCAGACCCCTGGCCTTGAACCAACTACCCTTCAACCCACTACTGTCCTGGAATCAGATGG AGAAAGACCTCCAAAACTGGAGTTTGCAGACAACCGAATTAAAACTCTGGATGAAAAATTAAGAAACTTG CTCTATCAGGAGCACAGCATCTCTAGCATCTATCCCGAGAGTCAGAAGGATACCCAAAGCATAGACTCTC CATTTTCTTCCTCTGCTGAAGATACCCTCTCCTGTCCAGTGACAGAAGTCATAGCCATCAGTCACTGTGG AATTAAAGATAGCCCTGTACAATCCCCTAATTTCCAACAGACAGGCTCTAAGCTTCTGTCCAATGTGGCT GCAAGTCAGCCTGCTAATATATCAGTGTTCAAAAGGGACCTGAATGTGATAACTTCTGTACCCAGCGAAT TGTGTTTACATGAGATGTCCTCAGATGCTTCACTTCCAGGGGATCCAGAGGCCTATCCTGCTGCTGTGTC AAGCGGTGGAGCCATTCATCTGCAGACAGGAGGTGGATATTTTGGCCTAAGCTTTACTTGTCCTAGTCTC AAAAATCCTATTAGCAAGAAATCCTGGACTCGCAAATTAAAAAGCTGGGCATACAGGCTACGGCAGTCAA CCAGCTTTTTCAAGAGATCAAAAGTCCGTCAAGTGGAAACAGAAGAGATGAGATCAGCAATTGCTCCTGA TCCCATTCCTCTGACACGGGAGTCCACAGCTGATACTAGGGCTTTGAATAGATGTAAAGCGATGAGTGGA TCATTTCAGCGGGGTCGGTTCCAGGTGATTACAATTCCTCAGCAGCAGTCAGCAAAAATGACATCTTTTG GAATAGAACACATATCAGTGTTCAGTGAGACAAACCATTCTAGTGAAGAAGCCTTTATTAAAACAGCAAA GTCTCAGTTGGTAGAAATAGAACCTGCCACACAAAATCCAAAAACTTCGTTTTCTTATGAGAAGTTACAA GCTCTTCAGGAAACCTGTAAAGAAAATAAAGGAGTTCCCAAACAAGGTGACAACTTCTTATCTTTCAGCG CAGCTTGTGAGACTGATGTATCTTCAGTGACCCCAGAAAAGGAATTTGAAGAAACTTCAGCCACAGGAAG TAGCATGCAGTCTGGATCTGAACTGTTGCTTAAAGAGAGAGAGATATTGACTGCTGGGAAACAGCCTAGC TCTGATAGTGAATTTTCAGCCAGTCTTGCTGGCAGTGGAAAGTCAGTGGCAAAGACTGGTCCAGAGAGTA ATCAGTGCTTACCACACCACGAAGAACAAGCTTATGCTCAAACACAGAGTTCACTCTTCTATTCGCCATC TTCCCCAATGAGCAGTGATGATGAATCAGAAATAGAGGATGAGGACTTGAAGGTGGAGCTTCAAAGATTA CGAGAAAAACACATTCAGGAGGTGGTAAATCTTCAAACCCAGCAGAATAAGGAGCTGCAGGAGCTCTATG AACGCCTTCGGTCAATTAAAGATAGCAAAACCCAATCTACTGAGATTCCTTTGCCACCTGCATCACCACG TCGACCAAGATCTTTCAAAAGCAAACTTCGAAGCCGCCCCCAGTCCTTGACACATGTGGACAATGGCATA GTTGCTACAGGGAAAAGCTGCCTTATAAATGAGTTGGAAAATCCACTGTGTGTGGAGAGTAATGCAGCAT CATGCCAACAGTCTCCAGCCAGTAAAAAAGGGATGTTCACAGATGACTTACACAAGCTGGTGGATGACTG GACAAAGGAAGCAGTAGGAAATTCTCTTATTAAGCCAAGTTTAAACCAACTTAAACAAAGTCAACACAAA CTAGAGACAGAAAACTGGAATAAAGTATCTGAAAATACTCCGTCTACTATGGGCTACACATCAACATGGA TTTCTTCTCTGTCCCAAATCCGTGGAGCTGTCCCAACTTCCTTGCCACAAGGACTCTCACTCCCTTCATT TCCTGGGCCATTATCATCATATGGAATGCCTCACGTTTGTCAGTATAATGCTGTGGCGGGGGCGGGGTAT CCAGTACAGTGGGTAGGAATTTCAGGAACAACACAACAATCTGTAGTAATTCCCGCCCAATCTGGGGGAC CATTCCAGCCAGGGATGAATATGCAGGCATTTCCAACTTCATCAGTGCAGAATCCTGCCACAATCCCTCC TGGTCCTAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214627 representing NM_020922
Red=Cloning site Green=Tags(s) MATDSGDPASTEDSEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSVEMTEDDK VAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKSTSEISKDCFKEKNEKEMEEEAEMKAVAT SPSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFY DSWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDN IFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSE CQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRVELAEEDD CSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTP IKKTREKKPAGCLEERRDSQCKSMGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHC SSVTGDNLSEAGAASVIHSDTSSQPSVAYSSNQTMGSQMVSNIPQAEVNVPGQIYSSQQLVGHYQQVSGL QKHSKLTQPQILPLVQGQSTVLPVHVLGPTVVSQPQVSPLTVQKVPQIKPVSQPVGAEQQAALLKPDLVR SLNQDVATTKENVSSPDNPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSTSGDNMVECQLETHNNKM VTFKFDVDGDAPEDIADYMVEDNFVLESEKEKFVEELRAIVGQAQEILHVHFATERATGVDSITVDSNSS QTGSSEQVQINSTSTQTSNESAPQSSPVGRWRFCINQTIRNRETQSPPSLQHSMSAVPGRHPLPSPKNTS NKEISRDTLLTIENNPCHRALFTSKSEHKDVVDGKISECASVETKQPAILYQVEDNRQIMAPVTNSSSYS TTSVRAVPAECEGLTKQASIFIPVYPCHQTASQADALMSHPGESTQTSGNSLTTLAFDQKPQTLSVQQPA MDAEFISQEGETTVNTEASSPKTVIPTQTPGLEPTTLQPTTVLESDGERPPKLEFADNRIKTLDEKLRNL LYQEHSISSIYPESQKDTQSIDSPFSSSAEDTLSCPVTEVIAISHCGIKDSPVQSPNFQQTGSKLLSNVA ASQPANISVFKRDLNVITSVPSELCLHEMSSDASLPGDPEAYPAAVSSGGAIHLQTGGGYFGLSFTCPSL KNPISKKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEEMRSAIAPDPIPLTRESTADTRALNRCKAMSG SFQRGRFQVITIPQQQSAKMTSFGIEHISVFSETNHSSEEAFIKTAKSQLVEIEPATQNPKTSFSYEKLQ ALQETCKENKGVPKQGDNFLSFSAACETDVSSVTPEKEFEETSATGSSMQSGSELLLKEREILTAGKQPS SDSEFSASLAGSGKSVAKTGPESNQCLPHHEEQAYAQTQSSLFYSPSSPMSSDDESEIEDEDLKVELQRL REKHIQEVVNLQTQQNKELQELYERLRSIKDSKTQSTEIPLPPASPRRPRSFKSKLRSRPQSLTHVDNGI VATGKSCLINELENPLCVESNAASCQQSPASKKGMFTDDLHKLVDDWTKEAVGNSLIKPSLNQLKQSQHK LETENWNKVSENTPSTMGYTSTWISSLSQIRGAVPTSLPQGLSLPSFPGPLSSYGMPHVCQYNAVAGAGY PVQWVGISGTTQQSVVIPAQSGGPFQPGMNMQAFPTSSVQNPATIPPGPK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020922 |
| ORF Size | 11353 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020922.4, NP_065973.2 |
| RefSeq Size | 7743 bp |
| RefSeq ORF | 5403 bp |
| Locus ID | 65267 |
| UniProt ID | Q9BYP7 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010] |
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